Co3 Defective Mucosal Production of Il-17a in Pediatric Celiac Disease

Scaleia, Raffaella La; Barba, Ma. Cristina Parada; Montuori, Monica; Nardo, Giovanni Di; Barrella, Guilherme Emerson; Nenna, Raffaella; Oliva, Salvatore; Mennini, Maurizio; Valitutti, Francesco; Petrarca, Laura; Camillo, Chiara Di; Mastrogiacomo, G.; Barbato, M.; Cucchiara, Salvatore; Bonamico, Margherita; Piccoli, Mara; Mainiero, Fabrizio; Palmieri, G.

doi:10.1016/s1590-8658(10)60546-4

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“…The expression of ETS1 and miR-326 genes in autoimmune diseases such as autoimmune thyroiditis, multiple sclerosis, type 1 diabetes, etc. has been evaluated in previous studies but no similar study has been performed on patients with celiac disease [17][18][19]. Gene expression studies which have assessed the contribution of genes to the pathophysiology of CD and the effect of GFD on this contribution are crucial in providing a deeper insight into CD development and management [20,21].…”

Section: Discussionmentioning

confidence: 99%

“…It is noteworthy that La Scaleia et al reported that the size and dynamics of circulating tissue T IL-17A+ cells are defective in celiac disease, and this defect has a negative effect on the efficiency of the epithelial barrier and is associated with mucosal damage in patients with CD. They concluded that the IL-17 cytokine family can also be effective in protecting tight junctions [17]. Therefore, it can be considered that if the increase in the level of IL-17A in GFD treated CD patients is not due to inadvertent consumption of gluten by them, it may be related to the protective effect of this cytokine on intestinal tight junctions, which needs to be confirmed in further studies.…”

Section: Discussionmentioning

confidence: 99%

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Elevated interleukin-17A levels despite reduced microRNA-326 gene expression in celiac disease patients under gluten-free diet

Nafari

Asri

Rostami-Nejad

et al. 2022

Romanian Journal of Internal Medicine

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Introduction: The microRNA-326 (miR-326) gene, by targeting ETS Proto-Oncogene 1 (ETS1), regulates the differentiation and interleukin-17A production of T helper 17 (Th17) cells. Celiac disease (CD) is an intestinal autoimmune disorder, in which the cascade of Th17 cells plays an important role in its pathogenicity. The aim of this study was to evaluate the expression changes of miR-326 and its two target genes ETS1 and IL-17A in celiac disease patients under a gluten-free diet (GFD). We expected the expression of miR-326 and IL-17A gene to decrease, and the expression of the ETS1 gene to increase, following the adherence to GFD. Methods: Peripheral blood samples of 40 CD patients under GFD (for more than 1 year) and 40 healthy individuals were collected. RNA was extracted, cDNA was synthesized and the miR-326, ETS1 and IL-17A gene expressions were evaluated by the quantitative polymerase real-time qPCR method. P-value ˂ 0.05 was considered statistically significant. Results: Although miR-326 mRNA expression was significantly lower in CD patients (P = 0.001), no significant difference was observed in ETS1 mRNA level between the two groups (P = 0.54), but IL-17A was significantly overexpressed in CD patients (P=0.002). No significant correlation was observed between the expression of the studied genes and the patients’ symptoms and Marsh classification. Conclusion:Adherence to the GFD for one to two years did not have the expected effect on the expression of genes in this panel. The most important finding that contradicted our hypothesis was the observation of high IL-17A levels in CD patients despite dieting, which may be related to the protective effect of this cytokine on intestinal tight junctions, that needs to be confirmed in further studies.

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