CO2 sequestration potential of halo-tolerant bacterium Pseudomonas aeruginosa SSL-4 and its application for recovery of fatty alcohols

Mishra, Somesh; Chauhan, Piyush; Gupta, Suresh; Raghuvanshi, Smita; Singh, Raju; Jha, Pallavi

doi:10.1016/j.psep.2017.08.013

Cited by 17 publications

(3 citation statements)

References 27 publications

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“…Microbial strain P. aeruginosa gi | KP 163922 | used in this study was previously isolated from the Sambhar salt lake near Jaipur, Rajasthan in a different study (Mishra et al 2017). The organic solvents, medium ingredients, and chemicals were purchased from HiMedia and Merck.…”

Section: Microorganism and Chemicalsmentioning

confidence: 99%

Biosurfactant Production by Free and Immobilized cells of Pseudomonas aeruginosa using Waste Engine Oil: A Comparative Analysis

Pradhan

Jujaru

Gaur

et al. 2022

Preprint

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This study investigated biosurfactant production by both free and immobilized bacterial strain of Pseudomonas aeruginosa using waste engine oil as a substrate. Polyurethane foam cubes were used as a carrier for the immobilization. Immobilization of cells using polyurethane foam was found to be convenient and after two days the bacterial growth was 2.9×1014 CFU/ml. The batch experiments were performed in Erlenmeyer flasks and monitored at every 24 hr interval for both free and immobilized cell systems. The microbial population was counted using the plate count method and the hydrocarbon degradation percentage was calculated to evaluate bacterial activity. Surface tension was measured at regular intervals to ensure the presence of biosurfactant. The maximum reduction was found to be 37 and 35 mN/m in free and immobilized cell systems, respectively. The chemical characterization using Fourier transform infrared spectroscopy confirmed the obtained product as rhamnolipid. Biosurfactant yield was found to be maximum in the case of immobilized system which was approximately 18 g/L. Scanning electron micrographs showed the potential of reusing the immobilized cells with polyurethane foam cubes.

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Section: Microorganism and Chemicalsmentioning

confidence: 99%

Biosurfactant Production by Free and Immobilized cells of Pseudomonas aeruginosa using Waste Engine Oil: A Comparative Analysis

Pradhan

Jujaru

Gaur

et al. 2022

Preprint

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“…Besides the rTCA and WL pathways and the well-known Calvin–Benson–Bassham (CBB), other processes involved in CO 2 metabolism have been recently discovered [ 6 ]. Fatty alcohols and acids biosynthesis in Pseudomonas aeruginosa and the reversed-pyruvate ferredoxin oxidoreductase (rPFOR)/pyruvate-formate-lyase-dependent (Pfl) carbon assimilation in Clostridium beijerincki are two examples of newly described mechanisms allowing bacteria other than chemolithotrophic to use inorganic carbon [ 7 , 8 , 9 ]. It has been demonstrated that the assimilation of CO 2 can give bacteria some advantages, such as dissipating the excess of reducing equivalents produced by metabolic processes or accumulating organic molecules as energy and carbon storage [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Inorganic Carbon Assimilation and Electrosynthesis of Platform Chemicals in Bioelectrochemical Systems (BESs) Inoculated with Clostridium saccharoperbutylacetonicum N1-H4

et al. 2023

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The need for greener processes to satisfy the demand of platform chemicals together with the possibility of reusing CO2 from human activities has recently encouraged research on the set-up, optimization, and development of bioelectrochemical systems (BESs) for the electrosynthesis of organic compounds from inorganic carbon (CO2, HCO3−). In the present study, we tested the ability of Clostridium saccharoperbutylacetonicum N1-4 (DSMZ 14923) to produce acetate and D-3-hydroxybutyrate from inorganic carbon present in a CO2:N2 gas mix. At the same time, we tested the ability of a Shewanella oneidensis MR1 and Pseudomonas aeruginosa PA1430/CO1 consortium to provide reducing power to sustain carbon assimilation at the cathode. We tested the performance of three different systems with the same layouts, inocula, and media, but with the application of 1.5 V external voltage, of a 1000 Ω external load, and without any connection between the electrodes or external devices (open circuit voltage, OCV). We compared both CO2 assimilation rate and production of metabolites (formate, acetate 3-D-hydroxybutyrate) in our BESs with the values obtained in non-electrogenic control cultures and estimated the energy used by our BESs to assimilate 1 mol of CO2. Our results showed that C. saccharoperbutylacetonicum NT-1 achieved the maximum CO2 assimilation (95.5%) when the microbial fuel cells (MFCs) were connected to the 1000 Ω external resistor, with the Shewanella/Pseudomonas consortium as the only source of electrons. Furthermore, we detected a shift in the metabolism of C. saccharoperbutylacetonicum NT-1 because of its prolonged activity in BESs. Our results open new perspectives for the utilization of BESs in carbon capture and electrosynthesis of platform chemicals.

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“…In the field of wastewater treatment, the former has been extensively studied, and the typical representative is algae [26,27]. In fact, the latter also plays an important role, and the typical representatives are ammonia oxidizing bacteria [28], nitrifying bacteria [29], hydrogen bacteria [30], sulphur bacteria [31], and iron bacteria [32]. An insufficient organic carbon source is often encountered in wastewater treatment [33,34], while the latter can use CO 2 in wastewater as the carbon source coupled with the energy that released during the oxidation of inorganic compounds such as NH 4 + -N, nitrite nitrogen, H 2 , H 2 S, and Fe 2+ .…”

Section: Introductionmentioning

confidence: 99%

Isolation and Identification of a Carbon-Fixing Bacteria Strain and Its Efficiency for Nitrogen and Phosphorus Removal from Viaduct Rainwater

Qing-jun

Deng

et al. 2023

Water

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In order to explore bacteria resources that are applicable for purification of viaduct rainwater, a carbon-fixing bacteria strain numbered 1C-1 was isolated from the sediment of a viaduct rainwater tank. The strain was identified through morphological characteristics and 16S rDNA sequences. The effects of three main factors (the simulated viaduct rainwater concentration, the carbon source dosage, and the inoculation amount) on the nitrogen and phosphorus removal rate of the strain were tested using simulated viaduct rainwater. Based on this, the nitrogen and phosphorus removal efficiencies for the actual viaduct rainwater were verified. The results showed that the strain belonged to Streptomyces sp. Under different simulated viaduct rainwater concentrations, the strain exhibited relatively high efficiency for nitrogen and phosphorus removal at the original concentration of simulated viaduct rainwater; other conditions remaining unchanged, the purification efficiency was relatively high when the glucose dosage was 800 mg, and the removal rates of ammonia nitrogen (NH4+-N), total nitrogen (TN), and total phosphorus (TP) were 71.48%, 47.86%, and 10.43%, respectively; other conditions remaining unchanged, the purification efficiency was relatively high when the inoculation amount was 1%, and the removal rates of NH4+-N, TN, and TP reached 58.62%, 58.35%, and 27.32%, respectively. Under the above optimal process conditions of an original concentration of viaduct rainwater, a carbon source dosage of 800 mg, and an inoculation amount of 1%, the strain removed 92.62%, 6.98%, and 6.16% of NH4+-N, TN, and TP, respectively from the actual viaduct rainwater; more interestingly, the removal rates of NH4+-N and TN were 43.26% and 78.02%, respectively, even without carbon source addition. It seems that there is no need for carbon source addition to remove nitrogen from the actual viaduct rainwater for the strain. To sum up, the carbon-fixing bacteria 1C-1 presents an obvious nitrogen and phosphorus removal effect (especially for nitrogen) for viaduct rainwater treatment and has application potential.

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CO2 sequestration potential of halo-tolerant bacterium Pseudomonas aeruginosa SSL-4 and its application for recovery of fatty alcohols

Cited by 17 publications

References 27 publications

Biosurfactant Production by Free and Immobilized cells of Pseudomonas aeruginosa using Waste Engine Oil: A Comparative Analysis

Biosurfactant Production by Free and Immobilized cells of Pseudomonas aeruginosa using Waste Engine Oil: A Comparative Analysis

Inorganic Carbon Assimilation and Electrosynthesis of Platform Chemicals in Bioelectrochemical Systems (BESs) Inoculated with Clostridium saccharoperbutylacetonicum N1-H4

Isolation and Identification of a Carbon-Fixing Bacteria Strain and Its Efficiency for Nitrogen and Phosphorus Removal from Viaduct Rainwater

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