CO2 Mediated Interaction in Yeast Stimulates Budding and Growth on Minimal Media

Abstract

Here we show that carbon dioxide (CO2) stimulates budding and shortens the lag-period of Saccharomyces cerevisiae cultures, grown on specific weak media. CO2 can be both exogenous and secreted by another growing yeast culture. We also show that this effect can be observed only in the lag-period, and demonstrate minimal doses and duration of culture exposition to CO2. Opposite to the effects of CO2 sensitivity, previously shown for pathogens, where increased concentration of CO2 suppressed mitosis and stimulated cell differentiation and invasion, here it stimulates budding and culture growth.

Revisiting the mitogenetic effect of ultra-weak photon emission

“…As Hollaender wrote, “It is doubly unfortunate that the problem has attracted some workers who, apparently, see in the problem only an opportunity to deal in the spectacular” (Hollaender, 1936 ). A number of phenomena initially attributed to MGE were later shown to be either artifacts or of purely chemical origin [e.g., quorum sensing factors (Hogan, 2006 ; Shank and Kolter, 2009 ), CO 2 (Hall et al, 2010 ; Volodyaev et al, 2013 ) or NH 3 -mediated interaction (Palková et al, 1997 )].…”

Development of advanced cellular and molecular biosensors for the study of neurotransmitter interaction and prospects for applications in Biology and Medicine

“…On the contrary, the experimental setup used in this work does not ensure that volatile compounds are not transferred between the cell populations. The likelihood of a distant gaseous cell-to-cell chemical interaction has previously been reported in accordance with the reported effect of CO2 on yeast cultures (Fels, 2016;Volodyaev et al, 2013). Nevertheless, given the results of the present work, it is relatively difficult to propose a volatile compound responsible for the observed NCDCI phenomenon, given the very small range of observed effect.…”

Physiology, ecology and industrial applications of aroma formation in yeast

“…Though not a distinguishable aroma for humans, other organisms have distinct sensory responses to carbon dioxide. In yeast populations, including S. cerevisiae , CO 2 can mediate cell–cell interactions, inducing growth and budding of neighboring colonies (Volodyaev, Krasilnikova and Ivanovsky 2013 ). In Candida albicans , increasing concentrations of self-generated CO 2 causes the cells to undergo morphological changes and switch to hyphal growth (Hall et al.…”