Application o f nano or biomaterials for enhanced oil recovery (EOR) has been recently much attended by petroleum engineering researchers. However, how would be the displacement mecha nisms and how would change the recovery efficiency while nano and biomaterials are used simultaneously is still an open question. To this end, a series o f injection tests performed on micromodel containing shale strikes. Three types o f biomaterials including biosurfactant, bioemulsifier, and biopolymer beside two types of nanoparticles includingSitfl and IIO2 at different concentrations were used as injection fluids. The microscopic as well as macro scopic efficiency of displacements were observed from analysis of images recorded during the tests. Microscopic observations revealed different mechanisms responsible for oil recovery includ ing: wettability alteration, thinning oil film, interfacial tension (IFT) reduction, and water in oil emulsion formation. Contact angle experiments showed changes in the surface wetness from an oil-wet to neutral-wet/water-wet conditions when a layer o f nano biomaterial covered thin sections of a shaly sandstone. Also the results showed that the presence of shales causes early break through and ultimate oil recovery reduction. Shales act as flow bar riers and enhance injection fluid viscous fingering. Displacement efficiency in shaly systems is sharply related to the shale distribu tion. Oil recovery after breakthrough in shaly systems is progres sive and considerable volume of oil in place is recovered after breakthrough. The highest efficiency, 78%, observed while injecting one pore volume of biopolymer and S1O2 nanoparticles. This work illustrates for the first time the mechanisms involved in nanobiomaterial-crude oil displacements.