CO2-enriched air in a temporary immersion system induces photomixotrophism during in vitro multiplication in vanilla

Spinoso-Castillo, José Luis; Bello-Bello, Jericó Jabín

doi:10.1007/s11240-023-02546-y

Cited by 6 publications

(3 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Plants coordinate their growth and development with carbon availability and source in vitro [30][31][32]. Understanding how sucrose levels (carbon source provided in the cultiva-…”

Section: Discussionmentioning

confidence: 99%

“…Plants coordinate their growth and development with carbon availability and source in vitro [30][31][32]. Understanding how sucrose levels (carbon source provided in the cultivation medium) and natural ventilation (carbon source provided in the flask headspace) can affect plant micropropagation offers new perspectives for producing plants with improved morphophysiological characteristics [19].…”

Section: Discussionmentioning

confidence: 99%

“…This may be the explanation for the greater biomass of Eryngium foetidum plants in a photomixotrophic without and with natural ventilation compared to photoautotrophic, as, often, these plants grown in a photoautotrophic system may not be achieving maximum photosynthesis performance due to extrinsic factors such as quantity (irradiance) and quality (spectral) of light, in addition to the low concentration of CO 2 in the headspace of the bottle. Based on this, CO 2 enrichment-or forced ventilation-has been applied to plant tissue culture (see [30][31][32]46]).…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Unlocking the Potential of In Vitro Photoautotrophy for Eryngium foetidum: Biomass, Morphophysiology, and Acclimatization

Silva,

Soares,

Silva

et al. 2024

Horticulturae

View full text Add to dashboard Cite

Eryngium foetidum L., a biennial herb with diverse applications in food and traditional medicine, holds economic and pharmacological significance. Given its growing commercial interest, implementing biotechnological approaches like plant tissue culture is vital for sustainable propagation and metabolite production. In this study, we investigated the in vitro photoautotrophic potential of Eryngium foetidum, examining growth, chlorophyll a fluorescence, photosynthetic pigments, and anatomical features under sucrose concentrations (0 and 30 g L−1) and gas exchange rate (14 and 25 μL L−1 s−1 CO2). Acclimatization and survival rates of plants after ex vitro transfer were also assessed. Eryngium foetidum exhibited robust growth in both photoautotrophic and photomixotrophic conditions, with natural ventilation significantly enhancing plant development. Chlorophyll a fluorescence and photosynthetic performance were influenced by sucrose and gas exchange, highlighting the importance of these factors in plant micropropagation. Moreover, the species demonstrated remarkable plasticity during acclimatization, with high survival rates and rapid inflorescence development. The research provides valuable insights into optimizing in vitro cultivation conditions for Eryngium foetidum, emphasizing the potential for large-scale clonal propagation and exploring secondary metabolites. The observed phenotypic plasticity underscores the adaptability of the species to diverse environments. These biotechnological strategies open avenues for future studies, including the application of elicitors for enhanced secondary metabolite production.

show abstract

“…Plants coordinate their growth and development with carbon availability and source in vitro [30][31][32]. Understanding how sucrose levels (carbon source provided in the cultiva-…”

Section: Discussionmentioning

confidence: 99%