Co-utilization of corn stover hydrolysates and biodiesel-derived glycerol by Cryptococcus curvatus for lipid production

Gong, Zhiwei; Zhou, Wenting; Shen, Hongwei; Zhao, Zongbao K.; Yang, Zhangqi; Yan, Jiabao; Zhao, Ming

doi:10.1016/j.biortech.2016.08.021

Cited by 61 publications

(27 citation statements)

References 34 publications

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“…Xylose consumption started until the glucose concentration dropped below 10.0 g/L, suggesting that the yeast strongly prefers glucose over xylose (Kim et al 2010). This phenomenon was in line with the results of C. curvatus and Mortierella isabellina on lignocellulosic hydrolysates (Ruan et al 2015;Gong et al 2016b). However, this finding was inconsistent with results reported for other oleaginous species such as Trichosporon cutaneum, Trichosporon fermentans, Thamnidium elegans, etc., probably due to the strain diversities (Hu et al 2011;Zikou et al 2013;Liu et al 2015).…”

Section: Compositional Analysis and Enzymatic Hydrolysis Of Waste Papersupporting

confidence: 72%

“…Lipid accumulation by C. curvatus generally occurs under nutrients limitation, preferably nitrogen starvation (Ratledge and Wynn 2002). It indicated that proteins were invalid nitrogen sources, probably because C. curvatus was unable to secrete proteases, which might be the major reason that lipid accumulation by C. curvatus could be triggered under relatively lower C/N molar ratios than other oleaginous yeasts (Li et al 2007;Angerbauer et al 2008;Gong et al 2016b). Overall, there was no need to remove the hydrolytic enzymes of the hydrolysates, which could simplify the processes.…”

Section: Compositional Analysis and Enzymatic Hydrolysis Of Waste Papermentioning

confidence: 99%

“…Total nitrogen concentration and C/N ratio were calculated according to the published equations (Gong et al 2016b). The crude proteins content was roughly calculated by multiplying Kjeldahl nitrogen by a factor of 6.25.…”

Section: Methodsmentioning

confidence: 99%

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Feasibility of Lipid Production from Waste Paper by the Oleaginous Yeast Cryptococcus curvatus

et al. 2017

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Waste paper was studied as a potential source for lipid production using the oleaginous yeast Cryptococcus curvatus for the first time. Three common types of waste paper, office paper, newspaper, and cardboard, were directly hydrolyzed by an enzyme cocktail to generate sugar-rich and nitrogen-limited hydrolysates. When these hydrolysates were used without any auxiliary nutrients by C. curvatus, the lipid content and lipid yield were higher than 50% and 200 mg/g, respectively. The nitrogen-rich enzyme cocktail exerted no negative effects on lipid production. Moreover, the integrated processes of enzymatic hydrolysis and lipid fermentation achieved comparable lipid yield to the separate hydrolysis and lipid production process. The resulting lipid samples had similar fatty acid compositional profiles to those of vegetable oils, which suggested their potential for biodiesel production. These findings strongly supported waste paper as appealing substrates for lipid production via oleaginous yeast, which provided cost-effective waste paper-to-lipids routes for sustainable biodiesel production.

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Section: Compositional Analysis and Enzymatic Hydrolysis Of Waste Papersupporting

confidence: 72%

Section: Compositional Analysis and Enzymatic Hydrolysis Of Waste Papermentioning

confidence: 99%

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Feasibility of Lipid Production from Waste Paper by the Oleaginous Yeast Cryptococcus curvatus

et al. 2017

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“…through biological processes [4,[12][13][14][15]. In terms of microbial oil production, many studies have reported the growth of microorganisms on pure glycerol, crude glycerol, and glycerol-glucose mixture to produce health-beneficial fatty acids, such as arachidonic acid and eicosapentaenoic acid, as well as mixed microbial oils as fuel precursors [14][15][16][17][18][19][20][21].…”

Section: Practical Applicationsmentioning

confidence: 99%

“…Indeed, as a biodegradable solvent, glycerol has been used to synthesize a number of bioproducts such as microbial oils (including essential polyunsaturated fatty acids), succinic acid, 1,3‐propanediol, and ethanol through biological processes . In terms of microbial oil production, many studies have reported the growth of microorganisms on pure glycerol, crude glycerol, and glycerol–glucose mixture to produce health‐beneficial fatty acids, such as arachidonic acid and eicosapentaenoic acid, as well as mixed microbial oils as fuel precursors .…”

Section: Introductionmentioning

confidence: 99%

Co‐utilization of acidified glycerol pretreated‐sugarcane bagasse for microbial oil production by a novel Rhodosporidium strain

Hassanpour

Cai

Gebbie

et al. 2019

Engineering in Life Sciences

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Acidified glycerol pretreatment is very effective to deconstruct lignocellulosics for producing glucose. Co‐utilization of pretreated biomass and residual glycerol to bioproducts could reduce the costs associated with biomass wash and solvent recovery. In this study, a novel strain Rhodosporidium toruloides RP 15, isolated from sugarcane bagasse, was selected and tested for coconversion of pretreated biomass and residual glycerol to microbial oils. In the screening trails, Rh. toruloides RP 15 demonstrated the highest oil production capacity on glucose, xylose, and glycerol among the 10 strains. At the optimal C:N molar ratio of 140:1, this strain accumulated 56.7, 38.3, and 54.7% microbial oils based on dry cell biomass with 30 g/L glucose, xylose, and glycerol, respectively. Furthermore, sugarcane bagasse medium containing 32.6 g/L glucose from glycerol‐pretreated bagasse and 23.4 g/L glycerol from pretreatment hydrolysate were used to produce microbial oils by Rh. toruloides RP 15. Under the preliminary conditions without pH control, this strain produced 7.7 g/L oil with an oil content of 59.8%, which was comparable or better than those achieved with a synthetic medium. In addition, this strain also produced 3.5 mg/L carotenoid as a by‐product. It is expected that microbial oil production can be significantly improved through process optimization.

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Co‐utilization of amino acid‐rich wastes and glycerol for microbial lipid production

Kamal

Huang

Wang

et al. 2021

Biofuels Bioprod Bioref

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Microbial lipids are considered to be potential raw materials for biodiesel production. However, the costs of such a process remain high due to the use of expensive feedstocks for microbial lipid production. The meat industry and biodiesel refineries generate amino acid (AA) wastes and crude glycerol, respectively, which could be explored as relatively cheap feedstocks for oleaginous yeasts. Unfortunately, AA wastes have a high nitrogen content and crude glycerol contains several impurities, discouraging microbial lipid production. Here, glycerol was co-used with AA, or with AA blends with compositional profiles matching those of different meats, so that the media had favorable carbon-tonitrogen (C/N) ratios to promote AA conversion into lipids using the oleaginous yeast Cryptococcus curvatus ATCC 20509 under two-stage culture conditions. Co-utilization of glycerol and AA resulted in improved lipid production. The highest lipid yield of 20.9 g 100 g -1 was achieved with lysine and glycerol, where the lipid yield was 13.9 g 100 g -1 , using an AA blend of similar composition to sheep viscera and glycerol. The lipid products comprised over 93% of 16 and 18 carbon-containing fatty acids, showing high potential for biodiesel production. The co-use of glycerol with AA improved AA conversion into lipids while decreasing the inhibitory effects at relatively high AA concentrations. The utilization of crude glycerol and AA-rich wastes from the meat industry could help both the development of biorefineries and waste minimization.

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Co-utilization of corn stover hydrolysates and biodiesel-derived glycerol by Cryptococcus curvatus for lipid production

Cited by 61 publications

References 34 publications

Feasibility of Lipid Production from Waste Paper by the Oleaginous Yeast Cryptococcus curvatus

Feasibility of Lipid Production from Waste Paper by the Oleaginous Yeast Cryptococcus curvatus

Co‐utilization of acidified glycerol pretreated‐sugarcane bagasse for microbial oil production by a novel Rhodosporidium strain

Co‐utilization of amino acid‐rich wastes and glycerol for microbial lipid production

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