Co-Utilization of Canola Oil and Glucose on the Production of a Surfactant by Candida lipolytica

Abstract

Candida lipolytica synthesized a surfactant in a cultivation medium supplemented with canola oil and glucose as carbon sources. Measurements of biosurfactant production and surface tension indicated that the biosurfactant was produced at 48 h of fermentation. The surface-active species is constituted by the protein-lipid-polysaccharide complex in nature. The cell-free broth was particularly influenced by the addition of salt, the pH and temperature depending on the emulsified substrate (hexadecane or a vegetable oil). After comparison between ethyl acetate and mixtures of chloroform and methanol as solvent systems for surfactant recovery, it was found that ethyl acetate was able to extract crude surfactant material with high product recovery (8.0 g/L). The isolated biosurfactant decreased the surface tension to values of 30 mN/m at the critical micelle concentration. Emulsification properties of the biosurfactant produced were compared to those of commercial emulsifiers and other microbial surfactants.

Production and Characterization of a Biosurfactant fromCyberlindnera samutprakarnensisJP52T

“…There have been many reports on biosurfactant production for combination substrates of glucose and vegetable oil, e.g. glucose and corn oil utilized by C. bombicola, 23) glucose plus cotton seed oil by C. glabrata, 24) and glucose and canola oil by C. lipolytica, 25) but there are only few reports on biosurfactants production from palm oil, such as sophorolipid production by C. bombicola using palm oil and a methyl ester of palm oil. 26) Palm oil is a popular domestic plant and used as raw material for biodiesel production and so on.…”

“…Similar observations have been reported for another biosurfactant-producing yeast, C. lipolytica UCP0988 with 10% glucose plus 10% canola oil. 25) The kinetic behavior of C. samutprakarnensis JP52 T growth and biosurfactant production in GP medium were investigated. Cell biomass, biosurfactant activity as determined by the oil displacement test and surface tension reduction, substrate consumption determined as relative amounts of total reducing sugar, palm oil, and nitrate compound in culture broth are shown in Fig.…”

An Indigenous Biosurfactant Producing Burkholderia cepacia with High Emulsification Potential towards Crude Oil

“…An inexpensive hydrophobic materials sources like vegetable oils or waste cooking oil and carbohydrates is a good approach for reducing industrial waste generation [51]. B. cepacia KISRQC seems a promising strain for large-scale biosurfactant production since it can grow on no toxic material such as olive oil; which is an advantage from an industrial point of view.…”

Production of bioemulsifiers by Yarrowia lipolytica in sea water using diesel oil as the carbon source

“…The authors determined a surface tension reduction of 59% when palm oil was added at 2 kg/m 3 .day with a cycle time of 2 days/cycle. Rufino et al (2011) Sarubbo et al (2007), Candida lipolytica synthesized a surfactant in a cultivation medium supplemented with canola oil and glucose as carbon sources after 48 h of fermentation. The cellfree broth was particularly influenced by the addition of salt, the pH and temperature depending on the emulsified substrate (hexadecane or a vegetable oil).…”