Co-transformed, diploid Lolium perenne (perennial ryegrass), Lolium multiflorum (Italian ryegrass) and Lolium temulentum (darnel) plants produced by microprojectile bombardment

Dalton, Susan; Bettany, A. J. E.; Timms, E.; Morris, Phillip

doi:10.1007/s002990050649

Cited by 67 publications

(50 citation statements)

References 17 publications

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“…While, the callus induction frequency of Kospeed and Kowinearly was very similar to Hwasan 101; therefore it could be expected that approximately 9 weeks old calli possibly the best explant for Korean Italian ryegrass cultivars in Agrobacterium-mediated genetic transformation system. Similarly, Dalton et al (1999) noticed that young cell suspension cultures (i.e. less than 10 weeks after initiation) appeared to be critical for regeneration of transgenic Italian ryegrass in particle bombardment genetic transformation system.…”

Section: Effect Of Callus Age On Transformationmentioning

confidence: 93%

“…The susceptibility of Italian ryegrass to gene manipulation has been well established by direct gene transfer methods such as particle bombardment (Ye et al 1997;Dalton et al 1999;Takahashi et al 2002) or by silicon-carbide fibermediated transformation (Dalton et al 1998). For the first time, Ye et al (1997) generated transgenic Italian ryegrass using embryogenic suspension cells as target for particle bombardment-mediated transformation system.…”

mentioning

confidence: 99%

“…Although these studies generated higher number of transgenic Italian ryegrass; unfortunately, cultivars from which transgenic plants were regenerated were not mentioned. Dalton et al (1998) also produced transgenic Italian ryegrass using silicon-carbide-fibermediated transformation system which is considered as simpler and less expensive comparative to microprojectile bombardment, however; in terms of efficiency of resources the microprojectile bombardment method is preferable since larger volume of cells can be treated at one time (Dalton et al 1998;Dalton et al 1999). Although direct gene transfer technology has been used successfully to produce transgenic Italian ryegrass, it has been claimed in several studies that transgenic plants obtained by direct gene transfer methods through protoplast or particle bombardment showed multiple copy insertions of the transgenes (Dalton et al 1995;Spangenberg et al 1995).…”

mentioning

confidence: 99%

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Genotypic variation of Agrobacterium-mediated transformation of Italian ryegrass

Lee¹,

Choi²,

Kim³

et al. 2010

Electron. J. Biotechnol.

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Section: Effect Of Callus Age On Transformationmentioning

confidence: 93%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Genotypic variation of Agrobacterium-mediated transformation of Italian ryegrass

Lee¹,

Choi²,

Kim³

et al. 2010

Electron. J. Biotechnol.

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“…Since the first transgenic Lolium perenne (perennial ryegrass) plants were generated by Spangenberg et al (1995), efforts in Lolium transformation have resulted in successful transformation of L. multiflorum, L. perenne and L. temulentum by biolistic delivery (Ye et al 1997;Dalton et al 1999) and Agrobacterium tumefaciens-mediated methods (Bettany et al 2003;Bajaj et al 2006;Cao et al 2006;Altpeter 2006;Sato and Takamizo 2006;Ge et al 2007;Zhang et al 2012). This has resulted in progress in the development of traits such as improved digestibility (Tu et al 2010), salt tolerance (Wu et al 2005) and cold tolerance (Hisano et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Agrobacterium-mediated transformation of Lolium rigidum Gaud.

Ran

Patron

et al. 2014

Plant Cell Tiss Organ Cult

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Lolium rigidum Gaud. is an annual grass grown for forage but also an economically damaging crop weed. A single genotype somatic embryogenic callus line, VLR1-60, was identified from a herbicide susceptible L. rigidum population, VLR1, and proved to be amenable to Agrobacterium tumefaciens-mediated transformation. Somatic embryogenic calli were continuously induced from the meristematic region of VLR1-60 plants multiplied in vitro and the basic tolerance level of VLR1-60 to hygromycin B was determined. A hygromycin phosphotransferase gene was used as a selectable marker for hygromycin B selection. Somatic embryogenic calli derived from in vitro grown vegetative tillers were co-cultivated with the A. tumefaciens strain EHA105 harbouring binary vector carrying reporter genes and selectable marker in the presence of acetosyringone for 3 days. Inoculated calli were recovered on callus proliferation medium containing Timentin TM but lacking hygromycin and were then subcultured onto media with hygromycin concentrations increased progressively through time for selection of transformed plant cells. Putative transgenic plants were recovered and integration of transgenes was confirmed by Southern hybridization analysis and by detection of DsRed or GUS activity in transgenic plants. The frequency of plant transformation was 1.3 %. The ability to transform L. rigidum will provide opportunities for functional characterization of genes to improve forage quality and increase our understanding of the evolution of herbicide resistance and of the basic genetics underlying traits that make L. rigidum a damaging crop weed.

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“…More recently, efficient systems for the transformation of ryegrass species have been developed using both biolistic methods (Spangenberg et al 1995;Ye et al 1997;Dalton et al 1999;Altpeter et al 2000) and agrobacterium-mediated transformation (Bettany et al 2003;Bajaj et al 2006;Cao et al 2006;sato & takamizo 2006). Examples of uses of these technologies are given in later sections.…”

Section: Genetically Modified (Gm) Forage Speciesmentioning

confidence: 99%

Future options and targets for pasture plant breeding in New Zealand

Williams

Easton

Jones

2007

New Zealand Journal of Agricultural Research

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Industries based on pastoral farming have increased their contribution to GDP from 13.5 to 17% since 1990 as the result of markedly intensified farming practices. In the future, we predict that this intensification will continue but, at the same time, there will be an emergence of an efficient, lower-input farming sector with almost no environmental footprint. Both sectors will require continuing input by pasture plant breeders. Over the past 20 years, development of pasture cultivars has become totally industry funded, with support from Crown funding for basic research. There have been several key advances in pasture plant breeding including new methods for using exotic germplasm and secondary gene-pools, modification of grass-endophyte associations, breeding for specific environments and the successful adoption of international breeding programmes. The emergence of genomics, marker-assisted selection (MAS) and genetic modification (GM) offer considerable promise for future development of pasture cultivars. Future grass breeding, aided by MAS and GM of both plants and endophytes, will place strong emphasis on feeding value for optimal animal performances, especially in intensive systems. There will also be development of grass types adapted to efficient, lower-input farming systems that will have minimal environmental A07026;

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Co-transformed, diploid Lolium perenne (perennial ryegrass), Lolium multiflorum (Italian ryegrass) and Lolium temulentum (darnel) plants produced by microprojectile bombardment

Cited by 67 publications

References 17 publications

Genotypic variation of Agrobacterium-mediated transformation of Italian ryegrass

Genotypic variation of Agrobacterium-mediated transformation of Italian ryegrass

Agrobacterium-mediated transformation of Lolium rigidum Gaud.

Future options and targets for pasture plant breeding in New Zealand

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