“…The fresh culture of CS was placed in 2 mL tubes together with glass beads, centrifuged for 1.5 min twice and freezedried, obtaining the preparation referred as CS + B. The efficiency of the cell disruption step was analyzed by chlorophyll extraction in acetone 80% for CR [34,42] and DMSO for CS [43] by comparing the absorption spectra of pigments extracted before or after a centrifugation step at 1000 ×g for 3 min where the pellet was discarded. This centrifugation step essentially removed intact cells from the CS + B and CR + B samples.…”