CO₂‐induced chloroplast movement in one cell‐layer moss leaves

Abstract

CO2‐induced chloroplast movement was reported in the monograph by Gustav Senn in 1908: unilateral CO2 supply to the one cell‐layered moss leaves induced the positively CO2‐tactic periclinal arrangement of chloroplasts. Here, using the model moss plant Physcomitrium patens, we examined basic features of chloroplast CO2‐tactic relocation with a modernized experimental system. The CO2 relocation was light‐dependent and, especially, CO2 relocation in red light was substantially dependent on photosynthetic activity. In blue light, CO2 relocation was mainly dependent on microfilaments while microtubule‐based movement was insensitive to CO2, whereas in red light, both cytoskeletons contributed redundantly to CO2 relocation. The CO2 relocation was observed not only when the two leaf surfaces were exposed to CO2‐free air versus CO2‐containing air, but also by exposing them physiologically relevant differences in CO2 concentrations. In the leaves placed on the surface of a gel sheet, chloroplasts avoided the gel side and positioned in the air‐facing surface, and this phenomenon was also shown to be photosynthesis dependent. Based on these observations, we propose a hypothesis that the threshold light intensity between the light‐accumulation and ‐avoidance responses of the photorelocation would be increased by CO2, resulting in the CO2‐tactic relocation of chloroplasts.

Physcomitrium patens response to elevated CO2 is flexible and determined by an interaction between sugar and nitrogen availability

“…Among bryophytes, hornworts have been explored to some extent for their unique pyrenoid‐based carbon concentration mechanism (Li et al ., 2017). However, despite their evolutionary and ecological importance, a thorough understanding of moss growth and physiology under eCO 2 conditions remains extremely limited, except for a few reports exploring eCO 2− dependent gene expression changes and CO 2 ‐tactic chloroplast movements (Shinde et al ., 2015; Sugiyama & Terashima, 2023). Although increased CO 2 is expected to result in higher biomass accumulation in mosses, previous data are sparse and inconsistent.…”