Co-silence of the coatomer β and v-ATPase A genes by siRNA feeding reduces larval survival rate and weight gain of cotton bollworm, Helicoverpa armigera

Mao, Jianjun; Zhang, Pei-Zhuo; Liu, Changyan; Zeng, Fanrong

doi:10.1016/j.pestbp.2014.11.013

Cited by 32 publications

(35 citation statements)

References 44 publications

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“…For instance, simultaneous silencing of two essential genes, β-actin ( ACT ) and Shrub ( SHR ), resulted in reduced mortality of CPB larvae compared with the mortality when the two genes were silenced individually 46 . In addition, studies conducted in the red flour beetle, T. castaneum , and the cotton bollworm, Helicoverpa armigera , found no indication of synergism when multiple genes were targeted simultaneously 47, 48 . Although these studies did not target insecticide resistance-related genes, they imply that targeting two genes simultaneously does not necessarily result in a more sensitive phenotype.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of a cytochrome P450 and a UDP-glycosyltransferase is associated with imidacloprid resistance in the Colorado potato beetle, Leptinotarsa decemlineata

Kaplanoglu

Chapman

Scott

et al. 2017

Sci Rep

105

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Current control of insect pests relies on chemical insecticides, however, insecticide resistance development by pests is a growing concern in pest management. The main mechanisms for insecticide resistance typically involve elevated activity of detoxifying enzymes and xenobiotic transporters that break-down and excrete insecticide molecules. In this study, we investigated the molecular mechanisms of imidacloprid resistance in the Colorado potato beetle, Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae), an insect pest notorious for its capacity to develop insecticide resistance rapidly. We compared the transcriptome profiles of imidacloprid-resistant and sensitive beetle strains and identified 102 differentially expressed transcripts encoding detoxifying enzymes and xenobiotic transporters. Of these, 74 were up-regulated and 28 were down-regulated in the resistant strain. We then used RNA interference to knock down the transcript levels of seven up-regulated genes in the resistant beetles. Ingestion of double-stranded RNA successfully knocked down the expression of the genes for three cytochrome P450s (CYP6BQ15, CYP4Q3 and CYP4Q7), one ATP binding cassette (ABC) transporter (ABC-G), one esterase (EST1), and two UDP-glycosyltransferases (UGT1 and UGT2). Further, we demonstrated that silencing of CYP4Q3 and UGT2 significantly increased susceptibility of resistant beetles to imidacloprid, indicating that overexpression of these two genes contributes to imidacloprid resistance in this resistant strain.

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Section: Discussionmentioning

confidence: 99%

Overexpression of a cytochrome P450 and a UDP-glycosyltransferase is associated with imidacloprid resistance in the Colorado potato beetle, Leptinotarsa decemlineata

Kaplanoglu

Chapman

Scott

et al. 2017

Sci Rep

105

View full text Add to dashboard Cite

show abstract

“…and 34% silencing, respectively, on day 10 (Mao et al, 2015). When treatment of 1.36 µg acetylcholinesterase siRNA was withdrawn after feeding up to 2 nd -instar, larvae attained normal growth, with comparable pupal weight to controls and successfully completed their life cycle.…”

Section: Systemic But Transient Silencingmentioning

confidence: 98%

Diet-delivered RNAi in Helicoverpa armigera – Progresses and challenges

Lim

Robinson

Jain

et al. 2016

Journal of Insect Physiology

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“…Transgenic plants engineered to express insect dsRNAs emerged as a potential technology after two independent groups proved the concept of applying RNAi to control agricultural insect pests (Baum et al, 2007; Mao et al, 2007). This approach has been developed to control lepidopteran, coleopteran and hemipteran agricultural pests (Katoch et al, 2013; Li et al, 2011; Paim et al, 2012), including Helicoverpa armigera in cotton (Mao et al, 2011; Mao et al, 2015; Qi et al, 2015; Chikate et al, 2016) and tobacco (Zhu et al, 2012; Xiong et al, 2013; Tian et al, 2015; Mamta, Reddy & Rajam, 2015), Diabrotica virgifera virgifera in maize (Baum et al, 2007; Fishilevich et al, 2016), Nilaparvata lugens in rice (Zha et al, 2011; Li et al, 2011; Yu et al, 2014; Qiu et al, 2016), Myzus persicae (Mao et al, 2015; Tzin et al, 2015) in Nicotiana benthamiana (Khan et al, 2013; Pitino et al, 2011) and Arabidopsis thaliana (Coleman, Pitino & Hogenhout, 2014; Li et al, 2015) and Sitobion avenae in wheat (Xu et al, 2014). However, the availability of methods that allow the screening and evaluation of candidate RNAi targets is a critical requisite for developing specific and efficient RNAi-based pest control.…”

Section: Introductionmentioning

confidence: 99%

RNA interference as a gene silencing tool to controlTuta absolutain tomato (Solanum lycopersicum)

Camargo

Barbosa

Possignolo

et al. 2016

PeerJ

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RNA interference (RNAi), a gene-silencing mechanism that involves providing double-stranded RNA molecules that match a specific target gene sequence, is now widely used in functional genetic studies. The potential application of RNAi-mediated control of agricultural insect pests has rapidly become evident. The production of transgenic plants expressing dsRNA molecules that target essential insect genes could provide a means of specific gene silencing in larvae that feed on these plants, resulting in larval phenotypes that range from loss of appetite to death. In this report, we show that the tomato leafminer (Tuta absoluta), a major threat to commercial tomato production, can be targeted by RNAi. We selected two target genes (Vacuolar ATPase-A and Arginine kinase) based on the RNAi response reported for these genes in other pest species. In view of the lack of an artificial diet for T. absoluta, we used two approaches to deliver dsRNA into tomato leaflets. The first approach was based on the uptake of dsRNA by leaflets and the second was based on “in planta-induced transient gene silencing” (PITGS), a well-established method for silencing plant genes, used here for the first time to deliver in planta-transcribed dsRNA to target insect genes. Tuta absoluta larvae that fed on leaves containing dsRNA of the target genes showed an ∼60% reduction in target gene transcript accumulation, an increase in larval mortality and less leaf damage. We then generated transgenic ‘Micro-Tom’ tomato plants that expressed hairpin sequences for both genes and observed a reduction in foliar damage by T. absoluta in these plants. Our results demonstrate the feasibility of RNAi as an alternative method for controlling this critical tomato pest.

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Co-silence of the coatomer β and v-ATPase A genes by siRNA feeding reduces larval survival rate and weight gain of cotton bollworm, Helicoverpa armigera

Cited by 32 publications

References 44 publications

Overexpression of a cytochrome P450 and a UDP-glycosyltransferase is associated with imidacloprid resistance in the Colorado potato beetle, Leptinotarsa decemlineata

Overexpression of a cytochrome P450 and a UDP-glycosyltransferase is associated with imidacloprid resistance in the Colorado potato beetle, Leptinotarsa decemlineata

Diet-delivered RNAi in Helicoverpa armigera – Progresses and challenges

RNA interference as a gene silencing tool to controlTuta absolutain tomato (Solanum lycopersicum)

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