Co-production of two new peptide antibiotics by a bacterial isolate Paenibacillus alvei NP75

Balaiah, Anandaraj; Vellaichamy, Adaikkalam; Kachman, Maureen; Selvamanikandan, Athinarayanan; Pegu, Shyamanta; Murugan, Vadivel

doi:10.1016/j.bbrc.2008.12.007

Cited by 20 publications

(6 citation statements)

References 23 publications

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“…It is ubiquitously present in the environment and has been isolated from a variety of sources, including cheese (1), fermented tomatoes (2), honey (3), rice plants (4), and soil (5). Many species in the genus Paenibacillus have been successfully used for agricultural, horticultural, industrial, and medical applications (6–8).…”

Section: Genome Announcementmentioning

confidence: 99%

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Draft Genome Sequences of Paenibacillus alvei A6-6i and TS-15

et al. 2013

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Section: Genome Announcementmentioning

confidence: 99%

“…Many species in the genus Paenibacillus have been successfully used for agricultural, horticultural, industrial, and medical applications (6–8). P. alvei was also reported to produce peptide antibiotics that affect a wide spectrum of Gram-positive and Gram-negative bacteria (2). …”

Section: Genome Announcementmentioning

confidence: 99%

Draft Genome Sequences of Paenibacillus alvei A6-6i and TS-15

et al. 2013

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“…The first report of antimicrobial peptide production from this bacteria was by Anandaraj et al [1], who isolated a strain from fermented tomato fruit and detected two antimicrobial peptides, Paenibacillin P and Paenibacillin N. Other species of Paenibacillus have been reported as antimicrobial peptide producers too. For example, polymyxins, LI-F complex, saltavalin and gatavalin are produced by different strains of Paenibacillus polymyxa [25].…”

Section: Introductionmentioning

confidence: 99%

Detection of secreted antimicrobial peptides isolated from cell-free culture supernatant of Paenibacillus alvei AN5

Alkotaini

Anuar

Kadhum

et al. 2013

Journal of Industrial Microbiology and Biotechnology

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An antimicrobial substance produced by the Paenibacillus alvei strain AN5 was detected in fermentation broth. Subsequently, cell-free culture supernatant (CFCS) was obtained by medium centrifugation and filtration, and its antimicrobial activity was tested. This showed a broad inhibitory spectrum against both Gram-positive and -negative bacterial strains. The CFCS was then purified and subjected to SDS-PAGE and infrared spectroscopy, which indicated the proteinaceous nature of the antimicrobial compound. Some de novo sequencing using an automatic Q-TOF premier system determined the amino acid sequence of the purified antimicrobial peptide as Y-S-K-S-L-P-L-S-V-L-N-P (1,316 Da). The novel peptide was designated as peptide AN5-1. Its mode of action was bactericidal, inducing cell lysis in E. coli ATCC 29522 and S. aureus, and non-cell lysis in both S. marcescens and B. cereus ATCC 14579. Peptide AN5-1 displayed stability at a wide range of pH values (2–12) and remained active after exposure to high temperatures (100 °C). It also maintained its antimicrobial activity after incubation with chemicals such as SDS, urea and EDTA.

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“…This is even more astonishing given that several bioactive compounds have been described from other Paenibacillus species (17)(18)(19)(20)(21)(22)(23)(24). Other entomopathogenic bacteria (25,26) produce and secrete many different pathogenic compounds, for example, proteins, antibiotics, or polypeptides.…”

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confidence: 99%

Low-Molecular-Weight Metabolites Secreted by Paenibacillus larvae as Potential Virulence Factors of American Foulbrood

Schild

Fuchs

Bode

et al. 2014

Appl Environ Microbiol

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The spore-forming bacterium Paenibacillus larvae causes a severe and highly infective bee disease, American foulbrood (AFB). Despite the large economic losses induced by AFB, the virulence factors produced by P. larvae are as yet unknown. To identify such virulence factors, we experimentally infected young, susceptible larvae of the honeybee, Apis mellifera carnica, with different P. larvae isolates. Honeybee larvae were reared in vitro in 24-well plates in the laboratory after isolation from the brood comb. We identified genotype-specific differences in the etiopathology of AFB between the tested isolates of P. larvae, which were revealed by differences in the median lethal times. Furthermore, we confirmed that extracts of P. larvae cultures contain low-molecular-weight compounds, which are toxic to honeybee larvae. Our data indicate that P. larvae secretes metabolites into the medium with a potent honeybee toxic activity pointing to a novel pathogenic factor(s) of P. larvae. Genome mining of P. larvae subsp. larvae BRL-230010 led to the identification of several biosynthesis gene clusters putatively involved in natural product biosynthesis, highlighting the potential of P. larvae to produce such compounds.

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Co-production of two new peptide antibiotics by a bacterial isolate Paenibacillus alvei NP75

Cited by 20 publications

References 23 publications

Draft Genome Sequences of Paenibacillus alvei A6-6i and TS-15

Draft Genome Sequences of Paenibacillus alvei A6-6i and TS-15

Detection of secreted antimicrobial peptides isolated from cell-free culture supernatant of Paenibacillus alvei AN5

Low-Molecular-Weight Metabolites Secreted by Paenibacillus larvae as Potential Virulence Factors of American Foulbrood

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