Co-Production of NDM-1 and OXA-232 by ST16
            <i>Klebsiella Pneumoniae</i>
            , Italy, 2016

Avolio, Manuela; Vignaroli, Carla; Crapis, Massimo; Camporese, Alessandro

doi:10.2217/fmb-2017-0041

Cited by 42 publications

(34 citation statements)

References 20 publications

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“…OXA-232 was first identified in France in 2013 from a K. pneumoniae isolate [9]. Since then, OXA232Kp have been discovered worldwide [10][11][12][13][14][15][16][17][18]. In this study, a nosocomial outbreak of epidemics OXA232Kp involving 61 patients was discovered in a teaching hospital in Shanghai, China.…”

Section: Discussionmentioning

confidence: 90%

“…In Europe and some Asia countries, the majority of OXA232Kp strains Fig. 1 The distribution of the OXA-232-producing K. pneumoniae isolates were ST14 and ST16 [10][11][12][13][14][15][16][17][18]. It has been confirmed that K. pneumoniae ST15 is the high-risk clones producing OXA-48, KPCs, CTX-M-15 and other ESBLs or carbapenemases.…”

Section: Discussionmentioning

confidence: 95%

“…Since then, OXA-232-producing K. pneumoniae (OXA232Kp) had been identified worldwide. These OXA232Kp isolates belong to ST14, ST15, ST16, ST17, ST147, ST231, ST307, and ST395 [10][11][12][13][14][15][16][17][18]. Almost all OXA232Kp isolates had plasmids with more than 99% sequence identity to pOXA-232, and had a size of 6.1-kb and carried the ColE-type backbone [9].…”

Section: Introductionmentioning

confidence: 99%

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Nosocomial spread of OXA-232-producing Klebsiella pneumoniae ST15 in a teaching hospital, Shanghai, China

Li¹,

Ma²,

Qin

et al. 2019

BMC Microbiol

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Background: The spread and outbreak of Enterobacteriaceae producing OXA-48-like carbapenemases have become more and more prevalent in China. Results: A total of 62 non-duplicated OXA-232-producing K. pneumoniae (OXA232Kp) were isolated between 2015 and 2017. An outbreak of OXA232Kp was observed in burn ICU. The 62 OXA232Kp isolates were all belongs to ST15 and categorized into two PFGE types (A and B). Type A was dominated of the isolates, which contained 61 clinical isolates and divided into 10 subtypes (A1-A10). In addition, most of OXA232Kp strains exhibited low-level carbapenems resistance. All strains carried a 6141 bp ColKP3 plasmid harboring the bla OXA-232 gene which is highly homologous to other bla OXA-232-bearing plasmids involved in other studies in eastern China. Conclusions: In this study, clone transmission of OXA232Kp ST15was observed. Highly significant homology among the bla OXA-232-bearing plasmids indicated the important role of the 6.1 kb ColE-like plasmid on the prevalence of bla OXA-232 gene in China.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Nosocomial spread of OXA-232-producing Klebsiella pneumoniae ST15 in a teaching hospital, Shanghai, China

Li¹,

Ma²,

Qin

et al. 2019

BMC Microbiol

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“…Previous reports exist of NDM-5–, OXA-48–, and CTX-M-5–producing KP ST16 in Denmark, the United Kingdom, and Spain, respectively [ 26–28 ]. Scarce reports of isolated cases in the Netherlands (NDM-5) [ 29 ], Italy (NDM-1 and OXA-232) [ 30 ], and Rio de Janeiro, Brazil (OXA-370) were also documented [ 31 ]. In contrast, ST16 KP has rarely been described harboring bla KPC , except for 2 isolates reported in Rio de Janeiro in 2008 and 2009 ( bla KPC-2 ) and 1 isolate in Israel.…”

Section: Discussionmentioning

confidence: 99%

An Emerging Clone, Klebsiellapneumoniae Carbapenemase 2–Producing K. pneumoniae Sequence Type 16, Associated With High Mortality Rates in a CC258-Endemic Setting

Andrey

Dantas

Martins

et al. 2019

Clinical Infectious Diseases

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Background Carbapenemase-producing K. pneumoniae have become a global priority, not least in low-middle income countries. Here, we report the emergence and clinical impact of a novel KPC-K. pneumoniae ST16 clone in a Clonal Complex (CC)258 endemic setting. Methods In a teaching Brazilian hospital, a retrospective cohort of adult KPC-KP bloodstream infections (BSI) cases (January 2014 to December 2016) was established to study the molecular epidemiology and its impact on outcome (30-day all-cause mortality). KPC-KP isolates were MLST-typed. Survival analysis between ST/CC groups and risk factors for fatal outcome (logistic regression) were evaluated. Representative isolates underwent whole genome sequencing (WGS), and had their virulence tested in a Galleria larvae model. Results One hundred sixty-five unique KPC-KP BSI cases were identified. CC258 was predominant (66%), followed by ST16 (12%). The overall 30-day mortality rate was 60%; in contrast, 95% of ST16 cases were fatal. Patient’s severity scores were high and baseline clinical variables were not statistically different across ST’s. In multivariate analysis, ST16 (OR 21.4; CI95% 2.3-202.8; p=0,008) and septic shock (OR 11.9; CI95% 4.2-34.1; p<0,001) were independent risk factors for fatal outcome. ST16 clone carried up to 14 resistance genes, including blaKPC-2 in an IncFIBpQIL plasmid, KL51 capsule and Yersiniabactin virulence determinants. ST16 clone was highly pathogenic in the larvae model. Conclusions Mortality rates were high in this KPC-KP BSI cohort, where CC258 is endemic. An emerging ST16 clone was associated with high mortality. Our results suggest that even in endemic settings, highly virulent clones can rapidly emerge demanding constant monitoring.

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“…ST16 has been reported worldwide, showing different antimicrobial resistance profiles. In 2011, K. pneumoniae ST16 harbouring CTX-M-15 was the cause of nosocomial infections in Denmark and Sweden [4], NDM-5-carrying ST16 K. pneumoniae was described in Denmark [5], and later the co-presence of NDM-1 and OXA-232 in ST16 K. pneumoniae was observed in Italy [6].…”

Section: Introductionmentioning

confidence: 99%

Genomics of Klebsiella pneumoniae ST16 producing NDM-1, CTX-M-15, and OXA-232

Espinal

Nucleo

Caltagirone

et al. 2019

Clinical Microbiology and Infection

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Objectives: Genomic characterization of the internationally spread sequence type (ST) 16 carbapenemresistant Klebsiella pneumoniae. Methods: The complete genomes of three carbapenem producing ST16 K. pneumoniae from Italian patients were analysed by single-nucleotide polymorphism-based phylogeny, core genome multilocus sequence typing, resistance, plasmid, and virulence content and compared with ten genomes of ST16 strains isolated in other countries. Plasmids carrying bla NDM-1 or bla OXA-232 carbapenemase genes were assembled and sequences were analysed. Results: The internationally spread ST16 K. pneumoniae clone showed variability in terms of distribution of NDM-1 and OXA-232 type carbapenemases. In some ST16 strains, up to six plasmids can be simultaneously present in the same cell, including ColE-like plasmids carrying bla OXA-232 and IncF plasmids carrying bla NDM-1. The differences observed in plasmid, resistance, and virulence content and core genome suggested that there is not a unique, highly conserved ST16 clone, but instead different variants of this lineage circulate worldwide. Conclusions: The ST16 K. pneumoniae clone has spread worldwide and may become a high-risk clone.

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Co-Production of NDM-1 and OXA-232 by ST16 Klebsiella Pneumoniae , Italy, 2016

Cited by 42 publications

References 20 publications

Nosocomial spread of OXA-232-producing Klebsiella pneumoniae ST15 in a teaching hospital, Shanghai, China

Nosocomial spread of OXA-232-producing Klebsiella pneumoniae ST15 in a teaching hospital, Shanghai, China

An Emerging Clone, Klebsiellapneumoniae Carbapenemase 2–Producing K. pneumoniae Sequence Type 16, Associated With High Mortality Rates in a CC258-Endemic Setting

Genomics of Klebsiella pneumoniae ST16 producing NDM-1, CTX-M-15, and OXA-232

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