Co-production of bioethanol and xylosaccharides from steam-exploded eucalyptus sawdust using high solid loads in enzymatic hydrolysis: Effect of alkaline impregnation

Rochón, Eloísa; Cabrera, María Noel; Scutari, Valentina; Cagno, Matías; Guibaud, Abigail; Martínez, Santiago; Böthig, Silvia; Guchin, Nikolai; Ferrari, Mario Daniel; Lareo, Claudia

doi:10.1016/j.indcrop.2021.114253

Cited by 29 publications

(12 citation statements)

References 40 publications

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“…However, for an economically viable distillation process, ethanol concentration in the fermentation solution typically needs to reach up to 5% ( v / v ) [ 56 ]. Therefore, increasing the solid load in enzymatic hydrolysis is necessary to attain a higher sugar concentration for ethanol fermentation [ 57 ]. Nonetheless, several technical challenges impede the widespread application of enzymatic hydrolysis with high solid loads.…”

Section: Resultsmentioning

confidence: 99%

Highly Efficient Production of Cellulosic Ethanol from Poplar Using an Optimal C6/C5 Co-Fermentation Strain of Saccharomyces cerevisiae

Xu,

Sun,

Wang

et al. 2024

Microorganisms

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Cellulosic ethanol is the key technology to alleviate the pressure of energy supply and climate change. However, the ethanol production process, which is close to industrial production and has a high saccharification rate and ethanol yield, still needs to be developed. This study demonstrates the effective conversion of poplar wood waste into fuel-grade ethanol. By employing a two-step pretreatment using sodium chlorite (SC)-dilute sulfuric acid (DSA), the raw material achieved a sugar conversion rate exceeding 85% of the theoretical value. Under optimized conditions, brewing yeast co-utilizing C6/C5 enabled a yield of 35 g/L ethanol from 10% solid loading delignified poplar hydrolysate. We increased the solid loading to enhance the final ethanol concentration and optimized both the hydrolysis and fermentation stages. With 20% solid loading delignified poplar hydrolysate, the final ethanol concentration reached 60 g/L, a 71.4% increase from the 10% solid loading. Our work incorporates the pretreatment, enzymatic hydrolysis, and fermentation stages to establish a simple, crude poplar waste fuel ethanol process, expanding the range of feedstocks for second-generation fuel ethanol production.

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Section: Resultsmentioning

confidence: 99%

Highly Efficient Production of Cellulosic Ethanol from Poplar Using an Optimal C6/C5 Co-Fermentation Strain of Saccharomyces cerevisiae

Xu,

Sun,

Wang

et al. 2024

Microorganisms

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“…Analysis of variance (ANOVA), regression equations, and optimization with the desirability function was conducted with a 95% confidence level (α = 0.05) using Statistica 8.0 software (Statsoft, Tulsa, OK, E.U.A.). The hydrolysis efficiency, glucan conversion, XS recovery, glucan conversion efficiency, and ethanol production parameters were calculated using equations previously reported [7,9].…”

Section: Statistical Analysis and Calculationsmentioning

confidence: 99%

“…Martin-Sampedro et al [15] achieved a maximum XS recovery of 19% using eucalypt under similar pretreatment severities, while Park et al [39] reached 33% using eucalyptus and NaOH-catalyzed steam explosion. On the other hand, Rochón et al [9] achieved an XS recovery of 82% (mainly as xylose), working at 180 • C for 10 min in a semicontinuous steam explosion reactor. In our work, XS recovery in the liquid fraction was influenced significantly by the pretreatment time and the T-t interaction at a 95% confidence level (p < 0.05).…”

Section: Pretreatment Of Eucalypt Sawdustmentioning

confidence: 99%

“…A wide variety of pretreatments have been used so far to breakdown the complex structure of the plant cell wall [6][7][8][9][10][11]. Steam explosion is known as an efficient, environmentally friendly, and generally chemically free and industrially scalable pretreatment with which the biomass structure is altered using high-pressure steaming followed by an explosive decompression [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

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Steam Explosion of Eucalyptus grandis Sawdust for Ethanol Production within a Biorefinery Approach

et al. 2023

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In this work, Eucalyptus grandis sawdust was subjected to steam explosion as the first step in cellulosic ethanol production within a biorefinery approach. The effect of the moisture content in the eucalypt sawdust (8 and 50%) and pretreatment process variables, such as temperature and residence time, were evaluated along with the influence of the water washing of steam-exploded solids on enzymatic hydrolysis and C6 fermentation yields. All other process streams were characterized to evaluate the recovery yield of valuable co-products. A recovery of nearly 100% glucans in the solid fraction and 60% xylans in the liquid fraction, mainly as partially acetylated oligomers, was obtained. The best enzymatic hydrolysis efficiencies (66–67%) were achieved after pretreatment at 205 °C for 10 min. The washing of pretreated sawdust with water improved the hydrolysis efficiencies and ethanol production yields by 10% compared to the unwashed pretreated solids under the same experimental condition. The highest ethanol yields were achieved after pretreatment of the sawdust with an 8% moisture content at 205 °C for 10 min, enzymatic hydrolysis at 13 wt% total solids with 25 FPU/g glucans, and fermentation with S. cerevisiae PE-2. In this case, 227 L ethanol and 40 kg total xylose (including xylo-oligomers) were obtained per ton of dry eucalypt sawdust.

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“…The latter tend to have longer reaction times, a high soda and peroxide charge, and low temperatures. The extracted xylosaccharides can be recovered by precipitation with ethanol and used for biofilm production [35,36].…”

Section: Alkaline Pretreatmentmentioning

confidence: 99%

Alternatives for the Management of Industrial Forest Waste: Energy, Bioethanol, and Cellulose Pulp

Clavijo¹,

Guigou²,

Cassella³

et al. 2023

Recycling Strategy and Challenges Associated With Waste Management Towards Sustaining the World

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Modern kraft pulp mills generate solid waste of 1–2% of incoming debarked wood. Given the size of these plants, with an annual production capacity of at least 1000,000 tons, each plant generates 20,000–30,000 dry tons of waste per year. The largest current use of these residues is for combustion in biomass boilers for steam and power generation. However, the conversion of biomass into biofuels and chemicals is gaining interest due to increasing demands for energy, limited sources of fossil fuels, and growing concerns about the environmental impact of greenhouse gas emissions. This chapter shows the laboratory-scale results of the use of eucalyptus wood wastes to obtain cellulose pulp by alkali pulping reinforced with hydrogen peroxide to obtain alkaline peroxide mechanical pulp or cellulosic bioethanol. Based on the results, an industrial-scale techno-economic analysis of the processes is presented and compared with current alternatives for energy generation.

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Co-production of bioethanol and xylosaccharides from steam-exploded eucalyptus sawdust using high solid loads in enzymatic hydrolysis: Effect of alkaline impregnation

Cited by 29 publications

References 40 publications

Highly Efficient Production of Cellulosic Ethanol from Poplar Using an Optimal C6/C5 Co-Fermentation Strain of Saccharomyces cerevisiae

Highly Efficient Production of Cellulosic Ethanol from Poplar Using an Optimal C6/C5 Co-Fermentation Strain of Saccharomyces cerevisiae

Steam Explosion of Eucalyptus grandis Sawdust for Ethanol Production within a Biorefinery Approach

Alternatives for the Management of Industrial Forest Waste: Energy, Bioethanol, and Cellulose Pulp

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