Co‐occurring increases of calcium and organellar reactive oxygen species determine differential activation of antioxidant and defense enzymes in <i>Ulva compressa</i> (Chlorophyta) exposed to copper excess

González, Alberto; Vera, Jeannette; Castro, Jorge; Dennett, Geraldine; Mellado, Macarena; Morales, Bernardo; Correa, Juan A.; Moenne, Alejandra

doi:10.1111/j.1365-3040.2010.02169.x

Cited by 64 publications

(70 citation statements)

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“…14 U. compressa was incubated with 5 μM of thapsigargin and the increase in intracellular calcium was detected using Fluo-3AM and confocal microscopy 13 and monitored every 15 min (Fig. 1A and B).…”

Section: Copper Induced the Release Of Intracellular Calcium From Endmentioning

confidence: 99%

“…13 Copper induced a triphasic release of calcium with maximal levels at 2, 3 and 12 h and a biphasic production of hydrogen peroxide with peaks at 3 and 12 h. Interestingly, the production of hydrogen peroxide occurred exclusively in organelles, i.e., mitochondria and chloroplasts. In addition, calcium and hydrogen peroxide act as signals in the differential activation of antioxidant and defense enzymes.…”

mentioning

confidence: 93%

“…In addition, calcium and hydrogen peroxide act as signals in the differential activation of antioxidant and defense enzymes. 13 In this work, we analyzed the intracellular origin of copper-induced calcium release and the type of calcium channels activated in response to copper excess in U. compressa.…”

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confidence: 99%

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Copper-induced calcium release from ER involves the activation of ryanodine-sensitive and IP3-sensitive channels inUlva compressa

González

Trebotich

Vergara

et al. 2010

Plant Signaling & Behavior

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T he marine alga Ulva compressa (Chlorophyta) showed a triphasic release of intracellular calcium with maximal levels at 2, 3 and 12 h and a biphasic accumulation of intracellular hydrogen peroxide with peaks at 3 and 12 h when cultivated with copper excess. Intracellular hydrogen peroxide originated exclusively in organelles. In this work, we analyzed the intracellular origin of calcium release and the type of calcium channels activated in response to copper excess. U. compressa was treated with thapsigargin, an inhibitor of endoplasmic reticulum (ER) calcium ATPase, ryanodine, an inhibitor of ryanodine-sensitive channels and xestospongin C, an inhibitor of inositol 1, 4, 5-triphosphate (IP 3 )-sensitive channels. Thapsigargin induced the depletion of calcium stored in ER at 75 min and completely inhibited calcium release at 2, 3 and 12 h of copper exposure indicating that calcium release originated in ER. In addition, ryanodine and xestospogin C inhibited calcium release at 2 and 3 h of copper exposure whereas the peak at 12 h was only inhibited by ryanodine. Thus, copper induced the activation of ryanodine-sensitive and IP 3 -sensitive calcium channels in ER of U. compressa.Plants showed common responses to biotic and abiotic stresses, mainly the accumulation of reactive oxygen species (ROS), in particular hydrogen peroxide, and the release of intracellular calcium.

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Section: Copper Induced the Release Of Intracellular Calcium From Endmentioning

confidence: 99%

mentioning

confidence: 93%

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Copper-induced calcium release from ER involves the activation of ryanodine-sensitive and IP3-sensitive channels inUlva compressa

González

Trebotich

Vergara

et al. 2010

Plant Signaling & Behavior

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“…Nicotiana plumbagnifolia, 17 Phaseolus vulgaris, 18 Ulva compressa, 19 Zea mays 20 or Arabidopsis thaliana. 21 This essentiality/toxic duality of transition metals has resulted in the development of a complex homeostatic network for their acquisition and use in aerobic organisms.…”

Section: Disclosure Of Potential Conflicts Of Interestmentioning

confidence: 99%

Transition metals: A double edge sward in ROS generation and signaling

Rodrigo-Moreno¹,

Poschenrieder²,

Shabala³

2013

Plant Signaling & Behavior

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Recently, reactive oxygen species (ROS) have emerged as important signaling molecules mediating a broad range of plant adaptive and developmental responses. In plant cells, both photosynthetic and respiratory electron-transport chains, as well as the NADPH oxidases and peroxidases are involved in ROS generation. 1 ROS have been shown to regulate gene expression and signaling transduction pathways and, as such, can control numerous processes, like root gravitropism, hypersensitive response to pathogens, stomatal closure and cell expansion and development. 1-5 During pathogen attack, programmed cell death (PCD) is induced in order to isolate cells and therefore avoiding pathogen spread. ROS play a crucial role in this signaling network. 6 In many cases, ROS production is genetically programmed and is induced during development. Generation of singlet oxygen induces controlled PCD in aleurone cells, leaf senescence, tracheary elements maturation or trichome development. 4 While ROS control over numerous adaptive and developmental responses is absolutely essential, a controlled balance of ROSproducing and ROS-scavenging systems must be kept to ensure an accurate execution of signaling without provoking toxicity. Several types of ROS may be formed in plant cells. These forms can be poorly reactive (non-radicals, such as H 2 O 2 or O 3 ) or can Transition metals such as iron (Fe) and Copper (Cu) are essential for plant cell development. At the same time, due their capability to generate hydroxyl radicals they can be potentially toxic to plant metabolism. Recent works on hydroxyl-radical activation of ion transporters suggest that hydroxyl radicals generated by transition metals could play an important role in plant growth and adaptation to imbalanced environments. in this mini-review, the relation between transition metals uptake and utilization and oxidative stress-activated ion transport in plant cells is analyzed, and a new model depicting both apoplastic and cytosolic mode of ROS signaling to plasma membrane transporters is suggested.

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“…Among such metals, copper is known to be a phytotoxic metal which reportedly induces a series of biological and chemical reactions in plant cells including the burst of reactive oxygen species production and stimulation of calcium channel opening allowing a transient increase in cytosolic calcium concentrations. [5][6][7][8] According to earlier works, ion of copper (chiefly Cu 2C ) was shown to bind and damage the DNA, 9,10 through binding to the guanine (G) and cytosine (C) bases at physiological pH. 11 In addition, among 3 of known biologically active forms of DNA, Z-DNA showing a non-WatsonCrick type structure, 12 possesses the highest affinity to binding of Cu 2C , especially at G bases.…”

mentioning

confidence: 99%