Co-localization of an endocytic marker and acid phosphatase in a tubular/reticular compartment in macrophages.

Luo, Zhongrong; Robinson, JM

doi:10.1177/40.1.1729356

Cited by 22 publications

(12 citation statements)

References 20 publications

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“…The speed of this process is apparent in the observation that within 10 min after cell activation, 60-80% of a pulse of endocytosed tracer is already contained within multivesicular bodies. We cannot exclude the possibility that this rapid movement represents flow of endocytosed material through a continuous system of tubules, with the apparent vesicles representing varicosities formed as the bolus of ingested material moves along the tubule, as has been suggested by others (33,34). Much of the initial internalization occurs in coated areas of membrane even though the uptake of these tracers is not mediated by a specific saturable receptor.…”

Section: Discussionmentioning

confidence: 81%

Internalization of type 1 complement receptors and de novo multivesicular body formation during chemoattractant-induced endocytosis in human neutrophils.

et al. 1994

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Section: Discussionmentioning

confidence: 81%

Internalization of type 1 complement receptors and de novo multivesicular body formation during chemoattractant-induced endocytosis in human neutrophils.

et al. 1994

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“…For each mouse strain, four independent samples per experiment were counted using the following characterization. AP stains the lysosomal compartment (27), and on the basis of the staining intensity and distribution we classified four different staining patterns: negative cells (granulocytes and myeloid precursors); weak cytoplasmic staining (cells with several small positive lysosomes, immature DC (28)); strong cytoplasmic staining (cells with strong AP activity in numerous lysosomes spread throughout the cytoplasm, typical for macrophages (29); and single-dot staining (mature DC with a dot-like AP-active region located in the cell center (28).…”

Section: Cytochemistrymentioning

confidence: 99%

Bone Marrow Precursors of Nonobese Diabetic Mice Develop into Defective Macrophage-Like Dendritic Cells In Vitro

Nikolić

Bunk

Drexhage

et al. 2004

The Journal of Immunology

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The NOD mouse spontaneously develops autoimmune diabetes. Dendritic cells (DC) play a crucial role in the autoimmune response. Previous studies have reported a defective DC generation in vitro from the NOD mouse bone marrow (BM), but a deviated development of myeloid precursors into non-DC in response to GM-CSF was not considered. In this study, we demonstrate several abnormalities during myeloid differentiation of NOD BM precursors using GM-CSF in vitro. 1) We found reduced proliferation and increased cell death in NOD cultures, which explain the previously reported low yield of DC progeny in NOD. Cell yield in NOR cultures was normal. 2) In a detailed analysis GM-CSF-stimulated cultures, we observed in both NOD and NOR mice an increased frequency of macrophages, identified as CD11c+/MHCII− cells with typical macrophage morphology, phenotype, and acid phosphatase activity. This points to a preferential maturation of BM precursors into macrophages in mice with the NOD background. 3) The few CD11c+/MHCIIhigh cells that we obtained from NOD and NOR cultures, which resembled prototypic mature DC, appeared to be defective in stimulating allogeneic T cells. These DC had also strong acid phosphatase activity and elevated expression of monocyte/macrophage markers. In conclusion, in this study we describe a deviated development of myeloid BM precursors of NOD and NOR mice into macrophages and macrophage-like DC in vitro. Potentially, these anomalies contribute to the dysfunctional regulation of tolerance in NOD mice yet are insufficient to induce autoimmune diabetes because they occurred partly in NOR mice.

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“…They have been reported in other cell types, including the secreting parietal cells in the gastric mucosa (Sugai et al 1985;Pettitt et al 1995Pettitt et al ,1996. They have been involved in endocytic processes as part of the endosomal compartment in several epithelial cells (Marsh et al 1986;Kobayashi and Robinson 1991;Tooze and Hollinshead 1991;Luo and Robinson 1992;Myers et al 1993;Sandborn and Bendayan 1996;van Deurs et al 1996). In this context, one should keep in mind that endothelial cells are a specialized form of epithelial cell in which endocytosis is mainly targeted to transcytosis.…”

Section: Figurementioning

confidence: 99%

Evidence of a Tubular System for Transendothelial Transport in Arterial Capillaries of the Rete Mirabile

Bendayan

Rasio

1997

J Histochem Cytochem.

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SUMMARYThe arterial endothelial cells of the rete capillaries of the eel were examined by transmission electron microscopy on thin sections, on freeze-fracture replicas, by scanning electron microscopy, after cytochemical osmium impregnation and perfusion with peroxidase. The study revealed the existence of membrane-bound tubules and vesicles that open at both the luminal and abluminal poles of the cell and at the level of the intercellular space. The tubules are straight or present successive dilations and constrictions. They branch in various directions and intrude deeply into the cell cytoplasm, forming a complex tubular network within the cell. Immunocytochemical techniques were applied on immersion-fixed tissues and on perfusion of the capillaries with albumin and insulin. These demonstrated that the tubular-vesicular system is involved in the transport of circulating proteins. Furthermore, protein A-gold immunocytochemistry has revealed the association of actin with the membranes of this system. On the basis of these results, we suggest that the transendothelial transport of serum proteins takes place by a transcytotic process through a membrane-bound tubular-vesicular system and is equivalent to the large pore system presumed from functional studies. (J Histochem Cytochem 45:1365-1378, 1997)

show abstract

Co-localization of an endocytic marker and acid phosphatase in a tubular/reticular compartment in macrophages.

Cited by 22 publications

References 20 publications

Internalization of type 1 complement receptors and de novo multivesicular body formation during chemoattractant-induced endocytosis in human neutrophils.

Internalization of type 1 complement receptors and de novo multivesicular body formation during chemoattractant-induced endocytosis in human neutrophils.

Bone Marrow Precursors of Nonobese Diabetic Mice Develop into Defective Macrophage-Like Dendritic Cells In Vitro

Evidence of a Tubular System for Transendothelial Transport in Arterial Capillaries of the Rete Mirabile

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