Co-localisation of markers for glycinergic and GABAergic neurones in rat nucleus of the solitary tract: Implications for co-transmission

Batten, Trevor; Pow, David V.; Saha, Sikha

doi:10.1016/j.jchemneu.2010.04.001

Cited by 16 publications

(11 citation statements)

References 112 publications

(157 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar colocalization results have been reported in the sea lamprey brain (Villar-Cerviño et al, 2008a) and the medulla of a salamander (Landwehr and Dicke, 2005) and with Gly/ GABA immunohistochemistry and/or immunohistochemistry and in situ hybridization in mammals (see Tanaka and Ezure, 2004;Crook et al, 2006;Batten et al, 2010). Thus, in the Siberian sturgeon, a proportion of Gly-ir reticular cells was also GABA-ir, as observed in other vertebrates.…”

Section: Major Gly-ir Rhombencephalic Populations Organized In Longitsupporting

confidence: 84%

“…However, the catecholaminergic cells of this lobe occupy dorsal periventricular regions (Adrio et al, 2002). This indicates that the vagal lobe of the Siberian sturgeon is neurochemically heterogeneous, as reported for the nucleus of the solitary tract of mammals (see Batten et al, 2010). The rich innervation by Gly-ir fibers observed in the SGN strongly suggests that some Gly-ir cells of the visceral lobe are projection neurons.…”

Section: Major Gly-ir Rhombencephalic Populations Organized In Longitmentioning

confidence: 53%

“…Thus, in the Siberian sturgeon, a proportion of Gly-ir reticular cells was also GABA-ir, as observed in other vertebrates. The finding of nerve terminals in the nucleus of the solitary tract of the rat that are doubly immunoreactive for Gly and the neuronal Gly transporter GLYT2, to GLYT2 and GABA or to GLYT2 and GAD67, but not for GLYT2 and the vesicular glutamate transporter vGLUT2 (Batten et al, 2010), clearly favors the use of Gly and GABA as cotransmitters. Accordingly, colocalization of GABA and Gly in cells of the Siberian sturgeon brain suggests the possibility that these neurons release both transmitters, which would increase the Gly receptor channel activation, as suggested by experiments with zebrafish Gly receptors (David-Watine et al, 1999;Imboden et al, 2001).…”

Section: Major Gly-ir Rhombencephalic Populations Organized In Longitmentioning

confidence: 99%

See 2 more Smart Citations

Distribution of glycine immunoreactivity in the brain of the Siberian sturgeon (Acipenser baeri): Comparison with γ-aminobutyric acid

2011

View full text Add to dashboard Cite

Glycine and γ-aminobutyric acid (GABA) are the main inhibitory neurotransmitters in the central nervous system (CNS) of vertebrates. Studies on the distribution of glycinergic neurons and fibers have been carried out mainly in rodents and lampreys. With the aim of discovering more about the early evolution of this system in vertebrates, we analyzed the distribution of glycine-immunoreactive (Gly-ir) neurons and fibers in the CNS of a basal ray-finned fish, the Siberian sturgeon (Chondrostei, Acipenseriformes), by use of immunohistochemical techniques. We also compared the distribution of glycine and GABA by the use of double-immunofluorescence techniques and confocal microscopy. Our results revealed the presence of Gly-ir cells in different regions of the CNS, such as olfactory bulbs, preoptic area, hypothalamus, thalamus, pretectum, optic tectum, tegmentum and rostral spinal cord, although most of the Gly-ir cells and the most intensely immunoreactive cells were located in the rhombencephalon, mainly in the octavolateral area and reticular formation. In addition, coronet cells of the basal hypothalamus and saccus vasculosus were Gly-ir. Glycinergic fibers coursed along most brain regions and were more abundant in the thalamus, hypothalamus, optic tectum, tegmentum, isthmic region, and basal rhombencephalon. The Mauthner cell perikaryon was richly innervated by Gly-ir boutons, as reported for teleosts. With regard to the colocalization of glycine and GABA, double-immunoreactive cells were located mainly in the rhombencephalon. The results enable us to conclude that the distribution of glycine in the sturgeon brain is more similar to that observed in lampreys than that observed in mammals.

show abstract

Section: Major Gly-ir Rhombencephalic Populations Organized In Longitsupporting

confidence: 84%

Section: Major Gly-ir Rhombencephalic Populations Organized In Longitmentioning

confidence: 53%

Section: Major Gly-ir Rhombencephalic Populations Organized In Longitmentioning

confidence: 99%

See 1 more Smart Citation

Distribution of glycine immunoreactivity in the brain of the Siberian sturgeon (Acipenser baeri): Comparison with γ-aminobutyric acid

2011

View full text Add to dashboard Cite

show abstract

“…To understand the rostrocaudal distribution of CB1 receptors in the gamma-aminobutyric acid (GABA)ergic and glutamatergic neurons, sections were compared with the Chemoarcitectonic Atlas of the Rat Brainstem [29] and divided into four rostrocaudal levels: most rostral, rostral, intermediate, and caudal. Similar divisions have been made in previous studies [30], [31]. The most rostral level was approximately between bregma –12.36 mm and bregma –12.84 mm and the rostral level was approximately between bregma –12.84 mm and bregma –13.56 mm.…”

Section: Methodssupporting

confidence: 85%

Cannabinoids Facilitate the Swallowing Reflex Elicited by the Superior Laryngeal Nerve Stimulation in Rats

et al. 2012

View full text Add to dashboard Cite

Cannabinoids have been reported to be involved in affecting various biological functions through binding with cannabinoid receptors type 1 (CB1) and 2 (CB2). The present study was designed to investigate whether swallowing, an essential component of feeding behavior, is modulated after the administration of cannabinoid. The swallowing reflex evoked by the repetitive electrical stimulation of the superior laryngeal nerve in rats was recorded before and after the administration of the cannabinoid receptor agonist, WIN 55-212-2 (WIN), with or without CB1 or CB2 antagonist. The onset latency of the first swallow and the time intervals between swallows were analyzed. The onset latency and the intervals between swallows were shorter after the intravenous administration of WIN, and the strength of effect of WIN was dose-dependent. Although the intravenous administration of CB1 antagonist prior to intravenous administration of WIN blocked the effect of WIN, the administration of CB2 antagonist did not block the effect of WIN. The microinjection of the CB1 receptor antagonist directly into the nucleus tractus solitarius (NTS) prior to intravenous administration of WIN also blocked the effect of WIN. Immunofluorescence histochemistry was conducted to assess the co-localization of CB1 receptor immunoreactivity to glutamic acid decarboxylase 67 (GAD67) or glutamate in the NTS. CB1 receptor was co-localized more with GAD67 than glutamate in the NTS. These findings suggest that cannabinoids facilitate the swallowing reflex via CB1 receptors. Cannabinoids may attenuate the tonic inhibitory effect of GABA (gamma-aminobuteric acid) neurons in the central pattern generator for swallowing.

show abstract

“…Since NTS contains glycinergic terminals/receptors, given that glycine inhibits arterial chemoreflex at the level of the NTS in the rat, and considering that A 2a adenosine receptors may activate glycine release from central neurons, it is possible that A 2a adenosine receptors may inhibit the CCR via facilitation of glycine release which then would inhibit transmission in this reflex pathway (Edwards and Robertson, 1999;Baptista and Varanda, 2005;Pimentel et al, 2003). It is also possible that adenosine A 2a receptors facilitate both GABA and glycine release as co-localization of glycinergic and GABA-ergic mechanisms in the NTS has been reported (Batten et al, 2010); however, only GABA but not glycine mediate CCR responses at the level of the rostral ventrolateral medulla (Verberne and Guyenet, 1992) and activation of NTS GABA A receptors inhibits the CCR reactivity (Sartor and Verberne, 2007). Which of those potential mechanisms is indeed responsible for A 2a receptor mediated inhibition of the CCR pathway remains to be elucidated in future studies.…”

Section: Discussionmentioning

confidence: 99%

Nucleus tractus solitarii A2a adenosine receptors inhibit cardiopulmonary chemoreflex control of sympathetic outputs

Minic¹,

O’Leary²,

Scislo³

2014

Autonomic Neuroscience

View full text Add to dashboard Cite

Previously we have shown that stimulation of inhibitory A1 adenosine receptors located in the nucleus tractus solitarii (NTS) attenuates cardiopulmonary chemoreflex (CCR) evoked inhibition of renal, adrenal and lumbar sympathetic nerve activity and reflex decreases in arterial pressure and heart rate. Activation of facilitatory A2a adenosine receptors, which dominate over A1 receptors in the NTS, contrastingly alters baseline activity of regional sympathetic outputs: it decreases renal, increases adrenal and does not change lumbar nerve activity. Considering that NTS A2a receptors may facilitate release of inhibitory transmitters we hypothesized that A2a receptors will act in concert with A1 receptors differentially inhibiting regional sympathetic CCR responses (adrenal>lumbar>renal). In urethane/chloralose anesthetized rats (n=38) we compared regional sympathetic responses evoked by stimulation of the CCR with right atrial injections of serotonin 5HT3 receptor agonist, phenylbiguanide, (1–8 µg/kg) before and after selective stimulation, blockade or combined blockade and stimulation of NTS A2a adenosine receptors (microinjections into the NTS of CGS-21680 0.2–20 pmol/50 nl, ZM-241385 40 pmol/100 nl or ZM-241385+CGS-21680, respectively). We found that stimulation of A2a adenosine receptors uniformly inhibited the regional sympathetic and hemodynamic reflex responses and this effect was abolished by the selective blockade of NTS A2a receptors. This indicates that A2a receptor triggered inhibition of CCR responses and the contrasting shifts in baseline sympathetic activity are mediated via different mechanisms. These data implicate that stimulation of NTS A2a receptors triggers unknown inhibitory mechanism(s) which in turn inhibit transmission in the CCR pathway when adenosine is released into the NTS during severe hypotension.

show abstract

Co-localisation of markers for glycinergic and GABAergic neurones in rat nucleus of the solitary tract: Implications for co-transmission

Cited by 16 publications

References 112 publications

Distribution of glycine immunoreactivity in the brain of the Siberian sturgeon (Acipenser baeri): Comparison with γ-aminobutyric acid

Distribution of glycine immunoreactivity in the brain of the Siberian sturgeon (Acipenser baeri): Comparison with γ-aminobutyric acid

Cannabinoids Facilitate the Swallowing Reflex Elicited by the Superior Laryngeal Nerve Stimulation in Rats

Nucleus tractus solitarii A2a adenosine receptors inhibit cardiopulmonary chemoreflex control of sympathetic outputs

Contact Info

Product

Resources

About