Co-inoculation of Lolium perenne with Funneliformis mosseae and the dark septate endophyte Cadophora sp. in a trace element-polluted soil

Berthelot, Charlotte; Blaudez, Damien; Béguiristain, Thierry; Chalot, Michel; Leyval, Corinne

doi:10.1007/s00572-018-0826-z

Cited by 37 publications

(20 citation statements)

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“…was neutral for the endomycorrhizal fungus, and the root colonization by Cadophora sp. was slightly reduced (Berthelot et al 2018). In planta, an antagonistic effect of the ECM fungus Laccaria bicolor on several DSE strains of Phialocephala sp.…”

Section: Fig 2: Outcomes Of the Interactions Between Ectomycorrhizalmentioning

confidence: 93%

Interactions between dark septate endophytes, ectomycorrhizal fungi and root pathogens in vitro

Berthelot

Leyval

Chalot

et al. 2019

FEMS Microbiology Letters

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Dark septate endophytes (DSEs) are widely distributed worldwide and can promote plant growth. Therefore, they are considered potentially important plant allies, especially in stressful environments. Previous studies have reported that DSEs cohabit roots with other microorganisms such as ectomycorrhizal (ECM), endophytic and pathogenic fungi/oomycetes. However, interactions between different DSE species have not yet been reported, and studies on the interactions between DSEs and other fungi are scarce. Using a simple and reproducible pairwise growth assay in vitro, we studied the synergistic/antagonistic interactions between eight DSEs, two ECM fungi and three root pathogens. Most of the DSE/DSE outcomes were neutral. Interestingly, we identified several DSE strains acting in synergy with other strains, as well as strains that could potentially act as biocontrol agents. Notably, three metal-tolerant DSE strains, namely, Cadophora sp., Leptodontidium sp. and Phialophora mustea, could decrease the growth of the root phytopathogens Pythium intermedium, Phytophthora citricola and Heterobasidion annosum. The present data are discussed in the general context of the use of fungal consortia as inocula in the tree-based phytomanagement of marginal lands.

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Section: Fig 2: Outcomes Of the Interactions Between Ectomycorrhizalmentioning

confidence: 93%

Interactions between dark septate endophytes, ectomycorrhizal fungi and root pathogens in vitro

Berthelot

Leyval

Chalot

et al. 2019

FEMS Microbiology Letters

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“…The studies that have investigated the effect of DSE on AMF colonization have revealed two general patterns. Saravesi (2014) and Berthelot et al (2018) grown with Mucor species in uncontaminated soils (Ważny et al, 2018). Thus, the reports available suggest that endophytic fungi either have no effect or promote total AMF colonization, which is in agreement with the results I obtained for A. tridentata and P. secunda, respectively.…”

Section: Effects Of Dse Inoculation On Amf Colonizationsupporting

confidence: 89%

“…Independent of whether the symbiosis is always commensalistic or not, it would be valuable to estimate the carbon cost of maintaining the DSE fungus. Such estimates may help to explain the lack of effects on plant growth as well as the apparent absence of negative impacts on AMF observed with this and other studies (Arriagada et al, 2012;Berthelot et al, 2018). of 8 or 9 replications.…”

Section: Discussionmentioning

confidence: 60%

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A Symbiosis Between a Dark Septate Fungus, an Arbuscular Mycorrhiza, and Two Plants Native to the Sagebrush Steppe

Carpenter¹

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Plant roots form symbioses with various fungi, including arbuscular mycorrhizae (AMFs) and dark septate endophytes (DSEs). The symbiosis between plants and AMFs has been extensively studied and is generally considered to be mutualistic. Much less is known about the symbiosis between plants and DSE. In sagebrush habitats, DSEs are common, but their effects on the vegetation are unclear. As a first step to study these effects, I isolated and cultured a DSE from the roots of the shrub Artemisia tridentata. Based on partial sequences of five genes and phylogenetic analyses, the isolated fungus was a non-described species within the Darksidea or a closely related sister group. Subsequently, I performed experiments in vitro and in potted plants to determine the effect of the isolated DSE on root tissue integrity, colonization by the AMF Rhizophagus irregularis, and plant biomass. These experiments were conducted in two plant species, A. tridentata and the native grass Poa secunda. Plants were exposed to one of four treatments: no inoculation (-AMF-DSE), inoculation with the DSE isolate (-AMF+DSE), inoculation with R. irregularis (+AMF-DSE), and inoculation with both fungi (+AMF+DSE). Microscopic observations revealed that the DSE hyphae grew along the root surface and penetrated epidermal and cortical cells without damage to them. In A. tridentata, the hyphae also reached the stele. For both species, total DSE colonization in the –AMF+DSE treatment was similar to that in the +AMF+DSE treatment, indicating the presence of AMF did not alter DSE colonization. Inoculation with DSE did not affect total AMF colonization of A. tridentata; however, it increased total colonization of P. secunda from 16.9 (+5.6%) in the +AMF-DSE treatment to 42.6 (+2.9%) in the +AMF+DSE treatment. Also, in both species, the presence of the DSE more than doubled the frequency of AMF intraradical storage structures, which consisted of vesicles plus intraradical spores. These results suggest that via increases in AMF colonization, DSE could lead to a beneficial effect on the host plants. However, neither on its own nor through co-inoculation with AMF, did the DSE isolate affect plant biomass. Thus, under the two conditions tested, the symbiosis was commensalistic. Further work is needed to evaluate the symbiosis in settings that better mimic the natural environment.

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“…Recent studies [10][11][12] suggested that DSEs could survive in TE-contaminated environments through three mechanisms: (i) extracellular chelation to prevent TE penetration into cells, (ii) complexation and sequestration of TE in cellular compartments to inhibit their toxicity, and (iii) restoration of TE-induced injuries. However, numerous studies reported the beneficial inoculation of several plant species to cope with metal toxicity [13][14][15][16][17][18]. Several authors hypothesized that the high melanin-derived pigmentation could partially explain the relatively high TE tolerance of DSEs and their TE accumulation capacities in the mycelia of DSEs [19,20].…”

Section: Introductionmentioning

confidence: 99%

Unravelling the Role of Melanin in Cd and Zn Tolerance and Accumulation of Three Dark Septate Endophytic Species

et al. 2020

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Dark septate endophytes (DSEs) are often trace element (TE)-tolerant fungi and are abundant in TE-polluted environments. The production of melanin, a black polymer found in cell walls, was hypothesized by several authors to play a role in the TE tolerance of DSEs. To test this hypothesis, we established a series of experiments using albino strains and melanin inhibitors and examined the responses to Cd and Zn. Six DSEs belonging to genera Cadophora sp., Leptodontidium sp. and Phialophora mustea, were evaluated. The strains mainly produced 1,8-dihydroxynaphthalene (DHN) melanin whereas 3,4-dihydroxyphenylalanin melanin was also synthetized. Cd and Zn decreased melanin synthesis in most of the strains. A reduction in melanin concentration in hyphae through the use of tricyclazole, an inhibitor of DHN-melanin synthesis, did not reduce the tolerance of the strains to Cd and Zn. Similarly, albino mutants of Leptodontidium sp. were not more sensitive to Cd and Zn than the WT strain. Moreover, tricyclazole-treated colonies accumulated less Cd but more Zn compared to untreated colonies. The Cd and Zn contents of Leptodontidium albino strains were variable and similar to that of the WT. The results suggest that melanin production is not an important functional trait that contributes to Cd and Zn tolerance, but might contribute to Cd accumulation.

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Co-inoculation of Lolium perenne with Funneliformis mosseae and the dark septate endophyte Cadophora sp. in a trace element-polluted soil

Cited by 37 publications

References 83 publications

Interactions between dark septate endophytes, ectomycorrhizal fungi and root pathogens in vitro

Interactions between dark septate endophytes, ectomycorrhizal fungi and root pathogens in vitro

A Symbiosis Between a Dark Septate Fungus, an Arbuscular Mycorrhiza, and Two Plants Native to the Sagebrush Steppe

Unravelling the Role of Melanin in Cd and Zn Tolerance and Accumulation of Three Dark Septate Endophytic Species

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