Co-inoculation of Legumes with Azospirillum and Symbiotic Rhizobia

Vicario, Julio César; Gallarato, Lucas Antonio; Paulucci, Natalia Soledad; Perrig, Diego; Bueno, Miguel Ángel Beltrán; Dardanelli, Marta Susana

doi:10.1007/978-3-319-06542-7_22

Cited by 6 publications

(3 citation statements)

References 26 publications

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“…The authors observed that co-inoculation with A. brasilense Az39 promoted a greater efficiency in the Bradyrhizobium-soybean symbiosis when compared to the treatment of co-inoculation with the mutant (Az39 ipdC-) or the application of synthetic IAA and concluded that both the presence of Azospirillum and IAA biosynthesis by these bacteria are responsible for the positive effects of soybean co-inoculation with Bradyrhizobium and PGPR. Several other studies have linked phytohormone production to the successful interaction between rhizobia and legumes (Fukuhara et al, 1994;Srinivasan, Holl & Petersen, 1996;Vicario et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Effects of plant growth-promoting rhizobacteria on co-inoculation with Bradyrhizobium in soybean crop: a meta-analysis of studies from 1987 to 2018

Zeffa

Fantin

Koltun

et al. 2020

PeerJ

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Background The co-inoculation of soybean with Bradyrhizobium and other plant growth-promoting rhizobacteria (PGPR) is considered a promising technology. However, there has been little quantitative analysis of the effects of this technique on yield variables. In this context, the present study aiming to provide a quantification of the effects of the co-inoculation of Bradyrhizobium and PGPR on the soybean crop using a meta-analysis approach. Methods A total of 42 published articles were examined, all of which considered the effects of co-inoculation of PGPR and Bradyrhizobium on the number of nodules, nodule biomass, root biomass, shoot biomass, shoot nitrogen content, and grain yield of soybean. We also determined whether the genus of the PGPR used as co-inoculant, as well as the experimental conditions, altered the effect size of the PGPR. Results The co-inoculation technology resulted in a significant increase in nodule number (11.40%), nodule biomass (6.47%), root biomass (12.84%), and shoot biomass (6.53%). Despite these positive results, no significant increase was observed in shoot nitrogen content and grain yield. The response of the co-inoculation varied according to the PGPR genus used as co-inoculant, as well as with the experimental conditions. In general, the genera Azospirillum, Bacillus, and Pseudomonas were more effective than Serratia. Overall, the observed increments were more pronounced under pot than that of field conditions. Collectively, this study summarize that co-inoculation improves plant development and increases nodulation, which may be important in overcoming nutritional limitations and potential stresses during the plant growth cycle, even though significant increases in grain yield have not been evidenced by this data meta-analysis.

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Section: Discussionmentioning

confidence: 99%

Effects of plant growth-promoting rhizobacteria on co-inoculation with Bradyrhizobium in soybean crop: a meta-analysis of studies from 1987 to 2018

Zeffa

Fantin

Koltun

et al. 2020

PeerJ

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“…They were also reported to promote growth indirectly by protecting the plant against some fungal pathogens either by producing siderophores, antimicrobial metabolites, or by competing for nutrients and/or niches (O’Sullivan and O’Gara, 1992). Several studies report the use of endophytic bacteria as inoculants to promote nodulation, plant growth, and yields (Vessey, 2003; Tilak et al, 2006; Elkoca et al, 2007; Hung et al, 2007; Liu et al, 2010; César Vicario et al, 2015). Sharma et al (2012) confirm the presence of Pseudomonas spp.…”

Section: Introductionmentioning

confidence: 99%

Phenotypic and Genotypic Diversity Among Symbiotic and Non-symbiotic Bacteria Present in Chickpea Nodules in Morocco

Benjelloun

Alami²,

Douira

et al. 2019

Front. Microbiol.

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Environmental pollution problems and increased demand for green technologies in production are forcing farmers to introduce agricultural practices with a lower impact on the environment. Chickpea (Cicer arietinum) in arid and semi-arid environments is frequently affected by harsh environmental stresses such as heat, drought and salinity, which limit its growth and productivity and affect biological nitrogen fixation ability of rhizobia. Climate change had further aggravated these stresses. Inoculation with appropriate stress tolerant rhizobia is necessary for an environmentally friendly and sustainable agricultural production. In this study, endophytic bacteria isolated from chickpea nodules from different soil types and regions in Morocco, were evaluated for their phenotypic and genotypic diversity in order to select the most tolerant ones for further inoculation of this crop. Phenotypic characterization of 135 endophytic bacteria from chickpea nodules showed a wide variability for tolerance to heavy metals and antibiotics, variable response to extreme temperatures, salinity, pH and water stress. 56% of isolates were able to nodulate chickpea. Numerical analysis of rep-PCR results showed that nodulating strains fell into 22 genotypes. Sequencing of 16S rRNA gene of endophytic bacteria from chickpea nodules revealed that 55% of isolated bacteria belong to Mesorhizobium genus. Based on MLSA of core genes (recA, atpD, glnII and dnaK), tasted strains were distributed into six clades and were closely related to Mesorhizobium ciceri, Mesorhizobium opportunistum, Mesorhizobium qingshengii, and Mesorhizobium plurifarium. Most of nodulating strains were belonging to a group genetically distinct from reference Mesorhizobium species. Three isolates belong to genus Burkholderia of the class β- proteobacteria, and 55 other strains belong to the class γ- proteobacteria. Some of the stress tolerant isolates have great potential for further inoculation of chickpea in the arid and semiarid environments to enhance biological nitrogen fixation and productivity in the context of climate change adaptation and mitigation.

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“…Besides N-fixation, most of these bacteria harbor the molecular cascade for other functions and traits that promote plant growth. Moreover, several studies reported the positive effect of bacteria species having additional traits on various plant In addition, several studies demonstrated the beneficial effect of co-inoculation of free-living nitrogen-fixing bacteria with symbiotic N fixers in legumes, indicating that free-living fixers support nodulation and further stimulate plant growth [50][51][52]. Besides N-fixation, most of these bacteria harbor the molecular cascade for other functions and traits that promote plant growth.…”

Section: Nitrogen Fixation Serves As a Mechanism Against Nitrogen Def...mentioning

confidence: 99%

The Importance of Microbial Inoculants in a Climate-Changing Agriculture in Eastern Mediterranean Region

et al. 2020

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Climate change has gained importance due to its severe consequences for many aspects of life. Increasing temperature, drought and greenhouse gases affect directly or indirectly the productivity of agricultural and natural ecosystems as well as human health. The nutrient supply capacity of the soil is diminishing, while food requirements for the growing population are increasing. The ongoing application of agrochemicals results in adverse effects on ecosystem functioning and food chain. Now, more than ever, there is a need to mitigate the effects of agricultural activities on climate change using environmentally friendly techniques. The role of plant beneficial microorganisms on this global challenge is increasingly being explored, and there is strong evidence that could be important. The use of functional microbial guilds forms an alternative or even a supplementary approach to common agricultural practices, due to their ability to act as biofertilizers and promote plant growth. Application of microbial inocula has a significantly lower impact on the environment compared to chemical inputs, while the agricultural sector will financially benefit, and consumers will have access to quality products. Microbial inoculants could play an important role in agricultural stress management and ameliorate the negative impacts of climate change. This short review highlights the role of microbes in benefiting agricultural practices against climate-changing conditions. In particular, the main microbial plant growth-promoting functional traits that are related to climate change are presented and discussed. The importance of microbial inoculants’ multifunctionality is debated, while future needs and challenges are also highlighted.

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Co-inoculation of Legumes with Azospirillum and Symbiotic Rhizobia

Cited by 6 publications

References 26 publications

Effects of plant growth-promoting rhizobacteria on co-inoculation with Bradyrhizobium in soybean crop: a meta-analysis of studies from 1987 to 2018

Effects of plant growth-promoting rhizobacteria on co-inoculation with Bradyrhizobium in soybean crop: a meta-analysis of studies from 1987 to 2018

Phenotypic and Genotypic Diversity Among Symbiotic and Non-symbiotic Bacteria Present in Chickpea Nodules in Morocco

The Importance of Microbial Inoculants in a Climate-Changing Agriculture in Eastern Mediterranean Region

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