Co-infection with Mycobacterium bovis does not alter the response to bovine leukemia virus in BoLA DRB3*0902, genetically resistant cattle

Lützelschwab, Claudia; Forletti, Agustina; Cepeda, Rosana; Esteban, Eduardo; Confalonieri, O.; Gutiérrez, Silvina

doi:10.1016/j.rvsc.2016.09.002

Cited by 14 publications

(20 citation statements)

References 59 publications

(70 reference statements)

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“…None of the evaluated parameters were affected by infection with M. bovis. Moreover, although the number of analysed animals was limited, it was found that the reactivity to bovine tuberculin was not associated with any of the BoLA-DRB3 genotypes in the population studied [34].…”

Section: Discussionmentioning

confidence: 81%

“…However, before studying the effect of the BoLA-DRB3 genotype on the variables under study, we evaluated the effect of positivity in the tuberculin test on each variable. The viral and immunological parameters under study were not significantly affected by the reactivity to the tuberculin test [34]. Figure 1 shows the kinetics of the proviral load in the animals grouped according to the BoLA-DRB3 alleles.…”

Section: Resultsmentioning

confidence: 96%

“…The experimental calves were then classified according to the proviral load attained at the end of the experiment. Animals with < 20 proviral copies/30 ng of DNA at 178 dpi or < 200 proviral copies at 88 dpi were classified as having LPL, while calves with proviral load levels above these limits were considered to have HPL (Table 1) in the tuberculin test and the infection profile of the animals [34]. In contrast, the association between the BLV infection profile and BoLA-DRB3 genetics was statistically significant (p < 0.01).…”

Section: Resultsmentioning

confidence: 99%

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Early events following bovine leukaemia virus infection in calves with different alleles of the major histocompatibility complex DRB3 gene

Forletti

Lützelschwab

Cepeda

et al. 2020

Vet Res

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Cattle maintaining a low proviral load (LPL) status after bovine leukaemia virus (BLV) infection have been recognized as BLV controllers and non-transmitters to uninfected cattle in experimental and natural conditions. LPL has been associated with host genetics, mainly with the BoLA class II DRB3 gene. The aim of this work was to study the kinetics of BLV and the host response in Holstein calves carrying different BoLA-DRB3 alleles. Twenty BLV-free calves were inoculated with infected lymphocytes. Two calves were maintained uninfected as controls. Proviral load, total leukocyte and lymphocyte counts, anti-BLVgp51 titres and BLVp24 expression levels were determined in blood samples at various times post-inoculation. The viral load peaked at 30 days post-inoculation (dpi) in all animals. The viral load decreased steadily from seroconversion (38 dpi) to the end of the study (178 dpi) in calves carrying a resistance-associated allele (*0902), while it was maintained at elevated levels in calves with *1501 or neutral alleles after seroconversion. Leukocyte and lymphocyte counts and BLVp24 expression did not significantly differ between genetic groups. Animals with < 20 proviral copies/30 ng of DNA at 178 dpi or < 200 proviral copies at 88 dpi were classified as LPL, while calves with levels above these limits were considered to have high proviral load (HPL) profiles. All six calves with the *1501 allele progressed to HPL, while LPL was attained by 6/7 (86%) and 2/6 (33%) of the calves with the *0902 and neutral alleles, respectively. One calf with both *0902 and *1501 developed LPL. This is the first report of experimental induction of the LPL profile in cattle.

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Section: Discussionmentioning

confidence: 81%

Section: Resultsmentioning

confidence: 96%

Section: Resultsmentioning

confidence: 99%

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Early events following bovine leukaemia virus infection in calves with different alleles of the major histocompatibility complex DRB3 gene

Forletti

Lützelschwab

Cepeda

et al. 2020

Vet Res

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“…145,147 Furthermore, to date, the BoLA-DRB3*0902 allele has not been associated with susceptibility to other infectious agent, nor has it negatively affected production or reproduction traits. 137,153 Moreover, R alleles are associated with resistance to intramammary infection and higher production traits. 149,150 Thus, expanding the population of cattle harboring these alleles in order to control BLV infection would additionally increase resistance to mastitis.…”

Section: Control Of Blv Infection By Genetic Selection Of Resistant Cmentioning

confidence: 99%

Bovine leukemia virus: current perspectives

Juliarena¹,

Barrios²,

Lützelschwab³

et al. 2017

VAAT

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Enzootic bovine leukosis, caused by bovine leukemia virus (BLV), is the most common neoplasm of dairy cattle. Although beef and dairy cattle are susceptible to BLV infection and BLV-associated lymphosarcoma, the disease is more commonly detected in dairy herds, mostly because of the management practices in dairy farms. The pathogenicity of BLV in its natural host, the bovine, depends mainly on the resistance/susceptibility genetics of the animal. The majority of infected cattle are asymptomatic, promoting the extremely high dissemination rate of BLV in many bovine populations. The important productive losses caused by the BLV, added to the health risk of maintaining populations with a high prevalence of infection with a retrovirus, generates the need to implement control measures. Different strategies to control the virus have been attempted. The most effective approach is to identify and cull the totality of infected cattle in the herd. However, this approach is not suitable for herds with high prevalence of infection. At present, no treatment or vaccine has proven effective for the control of BLV. Thus far, the genetic selection of resistant animals emerges as a natural strategy for the containment of the BLV dissemination. In natural conditions, most of the infected, resistant cattle can control the infection, and therefore do not pass the virus to other animals, gradually decreasing the prevalence of the herd.

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“…A recent study also showed no association of the aforementioned allele with infection by Mycobacterium bovis (M. bovis), although this observation should be confirmed in a more extensive study. Moreover, it was shown that the ability of cattle carrying resistance-associated marker to control BLV and to progress to LPL phenotype was not altered by M. bovis coinfection [118].…”

Section: Trends and Advances In Veterinary Geneticsmentioning

confidence: 99%

Major Histocompatibility Complex-Associated Resistance to Infectious Diseases: The Case of Bovine Leukemia Virus Infection

Gutiérrez¹,

Esteban²,

Lützelschwab³

et al. 2017

Trends and Advances in Veterinary Genetics

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The major histocompatibility complex (MHC) is a polymorphic gene cluster of about 150 genes, present in all vertebrates. Many of these genes contribute to immunity. Particularly, MHC-encoded class I and class II molecules, which are typically highly polymorphic and polygenic, are central in defining the specificity of the adaptive immune response. Among the diversity of genes associated with disease resistance, MHC genes are particularly interesting as they are associated with resistance and susceptibility to a wide range of diseases, some of which produce important economic losses in livestock. Enzootic bovine leukosis is an infectious disease caused by the retrovirus bovine leukemia virus (BLV), with an important economic impact, mainly in dairy herds. In this chapter, MHC-associated genetic resistance to BLV is revised. Certain alleles of the bovine MHC (BoLA) class II locus have been found strongly associated with resistance to viral dissemination. Genetic selection of resistant animals emerges as a natural strategy for the control of infectious diseases, especially when there is no other alternative of control or prevention, as vaccines. Founded on this knowledge, a BLV control program based on selection of genetically resistant cattle was designed. The proof of concept indicates that this strategy is feasible to implement in dairy herds.

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Co-infection with Mycobacterium bovis does not alter the response to bovine leukemia virus in BoLA DRB3*0902, genetically resistant cattle

Cited by 14 publications

References 59 publications

Early events following bovine leukaemia virus infection in calves with different alleles of the major histocompatibility complex DRB3 gene

Early events following bovine leukaemia virus infection in calves with different alleles of the major histocompatibility complex DRB3 gene

Bovine leukemia virus: current perspectives

Major Histocompatibility Complex-Associated Resistance to Infectious Diseases: The Case of Bovine Leukemia Virus Infection

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