Co-Infection of Mosquitoes with Chikungunya and Dengue Viruses Reveals Modulation of the Replication of Both Viruses in Midguts and Salivary Glands of Aedes aegypti Mosquitoes

Coupanec, Alain Le; Tchankouo-Nguetcheu, Stéphane; Roux, Pierre; Khun, Huot; Huerre, Michel; Vargas, Ronald Enrique Morales; Enguehard, Margot; Lavillette, Dimitri; Missé, Dorothée; Choumet, Valérie

doi:10.3390/ijms18081708

Cited by 60 publications

(61 citation statements)

References 35 publications

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“…This is because mosquitoes can feed multiple times on more than one human host and require multiple bites per each gonotrophic cycle (Harrington et al, 2014). Concomitant infections were reported in studies by Göertz et al (2017), Rückert et al (2017), Le Coupanec et al (2017) and Vazeille et al (2010). However, their reports are conflicting on the aspect of vector competence.…”

Section: Discussionmentioning

confidence: 82%

“…Aegypti mosquitoes could be infected with and transmit all combinations of these viruses simultaneously. In a study by Le Coupanec et al (2017), it was demonstrated that mixed infection of Chikungunya and Dengue viruses facilitated viral replication in Aedes aegypti thus increasing in their numbers before exit. It is worth noting that arboviruses can share a geographical area or overlap.…”

Section: Resurgence or Emergence Of Epidemic Arboviral Diseasesmentioning

confidence: 99%

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Entomological Coinfections of Arboviruses Dengue and Chikungunya Viruses along the Coastline of Kenya

Ngala¹,

Chanasit²

2018

jmr

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Arthropod-borne disease outbreaks as a result of pathogen influx including arboviruses transmitted by strains of Aedes species occur periodically in varying spots in Kenya. However, there has been paucity of documented information on the Aedes species involved in transmission of different haplotypes of arboviruses. This study assessed for concomitant infection of arboviruses in different vectors. Aedes species were sampled by Bio gent sentinel trap. Mosquitoes were stunned at 4°C for 5 minutes; sorted according to site, species and sex. RNA was extracted using Trizole®. cDNA was generated using one step real time PCR. Proportions of vectors and arboviruses were analyzed by R-statistics. A total of 37, 220 mosquitoes of Aedes species were analyzed. Vectors were: Aedes aegypti formosus (23,265=62.5%), Aedes aegypti aegypti (4,931=13.2%), Aedes mcintoshi (3,557=9.56%), Aedes ochraceus (2,156=5.79%), Aedes pembaensis (2,049=5.51%), Aedes tricholabis (487=1.31%), Aedes albicosta (415=1.11%), Aedes fulgens (200=0.54%) and Aedes fryeri (160=0.43%). Dengue virus (DENV) had highest entomological infections at 79% (n=1910) while Chikungunya (CHIKV) at 21%. DENV-2 had the highest frequency at 54%, while DENV-4 with the least frequency at 6%. Only ECSA genotype of CHIKV was present. Concomitant infections of arboviral haplotypes in different clades of Aedes species occur along the coastal region. Aedes aegypti aegypti is incubates all serotypes of DENV and genotype of CHIK viruses thus the major vectors of arboviruses. This information is important as it gives knowledge on areas at high risk for arboviral disease outbreaks. Consecutively, up scaled survey and implementation of control and prevention measures taken appropriately.

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Section: Discussionmentioning

confidence: 82%

Section: Resurgence or Emergence Of Epidemic Arboviral Diseasesmentioning

confidence: 99%

Entomological Coinfections of Arboviruses Dengue and Chikungunya Viruses along the Coastline of Kenya

Ngala¹,

Chanasit²

2018

jmr

View full text Add to dashboard Cite

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“…Moreover, the effect of coinfections on mosquito physiology has been addressed just recently [2, 98, 99]. Thus, so far we only have a partial view on the processes involved in vector susceptibly and transmission for arboviruses.…”

Section: Discussionmentioning

confidence: 99%

“…During the extrinsic incubation period (EIP), a virus that enters the mosquito midgut and is able to pass into the hemolymph may infect fat body cells, trachea, hemocytes, ovaries, nerve tissue, and reach salivary glands [2, 18, 32, 33]. For an arbovirus to be transmitted to a new vertebrate host in a subsequent blood meal, it must first be secreted with the saliva by the acinar (epithelial) cells to the luminal side of the salivary glands.…”

Section: Infection Of a Aegypti Mosquitoesmentioning

confidence: 99%

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Molecular Basis for Arbovirus Transmission by <b><i>Aedes aegypti</i></b> Mosquitoes

et al. 2018

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Mosquitoes are considered the most important vectors for the transmission of pathogens to humans. Aedes aegypti is a unique species, not only by its highly anthropophilic and peridomestic habits but also because it can transmit an important variety of pathogenic viruses. Examples are dengue, yellow fever, chikungunya, Zika, and Mayaro viruses. After ingesting viremic blood, a wide range of mechanisms are activated in the mosquito to counteract viral infection. Nevertheless, these arboviruses possess strategies to overcome barriers in the mosquito and eventually reach the salivary glands to continue the transmission cycle. However, the infection and eventual transmission of arbovirus depends on multiple factors. The current review focuses in detail on the anatomic, physiological, and molecular characteristics of the mosquito A. aegypti that participate in response to a viral infection. In the past decades, the awareness of the importance of this mosquito as a disease vector and its impact on human health was largely recognized. We need to improve our comprehension of molecular mechanisms that determine the outcome of successful virus replication or control of infection for each arbovirus in the vector; this could lead to the design of effective control strategies in the future.

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Mosquito‐infecting virus Espirito Santo virus inhibits replication and spread of dengue virus

White

Ming

Mazzara

et al. 2020

Journal of Medical Virology

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The primary vector of dengue virus (DENV) is Aedes aegypti. The mosquito‐infecting virus, Espirito Santo virus (ESV), does not infect Vero (mammalian) cells and grows in mosquito (C6/36) cells without cytopathic effects. Effects of ESV infection on replication of DENV were explored in vitro and in vivo, analyzing protein, RNA genome expression, and plaque formation. ESV and DENV simultaneous coinfection did not block protein synthesis from either virus but did result in inhibition of DENV replication in mosquito cells. Furthermore, ESV superinfected with DENV resulted in inhibition of DENV replication and spread in A. aegypti, thus reducing vector competence. Tissue culture experiments on viral kinetics of ESV and DENV coinfection showed that neither virus significantly affects the replication of the other in Vero, HeLa, or HEK cells. Hence, ESV blocks DENV replication in insect cells, but not the mammalian cells evaluated here. Our study provides new insights into ESV‐induced suppression of DENV, a globally important pathogen impacting public health.

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Co-Infection of Mosquitoes with Chikungunya and Dengue Viruses Reveals Modulation of the Replication of Both Viruses in Midguts and Salivary Glands of Aedes aegypti Mosquitoes

Cited by 60 publications

References 35 publications

Entomological Coinfections of Arboviruses Dengue and Chikungunya Viruses along the Coastline of Kenya

Entomological Coinfections of Arboviruses Dengue and Chikungunya Viruses along the Coastline of Kenya

Molecular Basis for Arbovirus Transmission by <b><i>Aedes aegypti</i></b> Mosquitoes

Mosquito‐infecting virus Espirito Santo virus inhibits replication and spread of dengue virus

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