Co-induction of beta-galactosidase and the lactose-P-enolpyruvate phosphotransferase system in Streptococcus salivarius and Streptococcus mutans

Hamilton, Ian R.; Lo, Gar

doi:10.1128/jb.136.3.900-908.1978

Cited by 61 publications

(56 citation statements)

References 26 publications

(15 reference statements)

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“…It has been shown previously that the transport of lactose in S. sobrinus and S. tnutans requires the induction of the lactose PTS which consists of a specific EII"'= and a soluble factor called IIP" (1,11,42). Indeed, IIP'" was found to be present only in cellular extracts from lactose-grown cells.…”

Section: Derepression Ot Lll">° In the Mutant Strainsmentioning

confidence: 98%

Two functionally different glucose phosphotransferase transport systems in Streptococcus mutans and Streptococcus sobrinus

Néron

Vadeboncoeur

1987

Oral Microbiology and Immunology

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Spontaneous phosphotransferase negative mutants of Streptococcus mutans GS5‐2 (mutant GM500) and of Streptococcus sobrinus 27352 (mutant MM202) were isolated by screening for 2‐deoxyglucose resistance using lactose as the growth substrate. Measurements of phosphoenolpyruvate: phosphotransferase activities with isolated membranes and purified Enzyme I and HPr indicated that the mutant strains were defective at the level of membrane components of the phosphoenolpyruvate: mannose phosphotranferase system but still possessed phosphoenolpyruvate: glucose phosphotransferase activity. These results were corroborated by uptake studies performed with resting cells. Our results suggest that glucose is transported by 2 distinct phosphoenolpyruvate: phosphotransferase systems in S. mutans and S. sobrinus, one of which is also capable of transporting mannose. This glucose‐mannose transport system is involved in catabolite repression and inducer exclusion, and controls, by way of these mechanisms, the metabolism of lactose.

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Section: Derepression Ot Lll">° In the Mutant Strainsmentioning

confidence: 98%

Two functionally different glucose phosphotransferase transport systems in Streptococcus mutans and Streptococcus sobrinus

Néron

Vadeboncoeur

1987

Oral Microbiology and Immunology

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“…We studied the order of sugar utilization during growth of these strains in mixtures of sugars (glucose-I-lactose and fructose-I-lactose). In these mixtures, the growth of the wild-type strain is diauxic (8,33,34). When glucose-adapted cells of the mutant strains were grown in a medium containing a mixture of glucose and lactose, growth was never diauxic.…”

Section: Control Of Sugar Utilization In the Mutant Strainsmentioning

confidence: 99%

“…Lactose, melibiose and galactose are non-PTS sugars in S. salivarius 25975 and enzymes involved in their metabolism are inducible (8,34). Accordingly, the rate of acid production from these sugars by resting cells of the wild-type strain grown on glucose was almost zero (Table 2).…”

Section: Derepression Of Catabolic Enzymes In the Mutant Strainsmentioning

confidence: 99%

“…salivarius. a gram-positive oral bacterium, lactose entry is mainly catalyzed by a non-PTS transport mechanism (8). Growth of the bacterium in a broth containing a mixture of glucose and lactose resulted in a diauxic growth curve, glucose being consumed first (8,33).…”

mentioning

confidence: 99%

“…a gram-positive oral bacterium, lactose entry is mainly catalyzed by a non-PTS transport mechanism (8). Growth of the bacterium in a broth containing a mixture of glucose and lactose resulted in a diauxic growth curve, glucose being consumed first (8,33). Our previous work on the structure of the glucose PTS in this bacterium indicated that S. salivarius did not possess a III''''-protein that stimulates the phosphorylation of a-methylglucoside (aMG), as is the case in enterobacteria (33).…”

mentioning

confidence: 99%

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Control of sugar utilization in oral streptococci. Properties of phenotypically distinct 2‐deoxyglucose‐resistant mutants of Streptococcus salivarius

Gauthier

Bourassa

Brochu

et al. 1990

Oral Microbiology and Immunology

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The physiological and biochemical characterization of Streptococcus salivarius mutants isolated by positive selection for resistance to 0.5 mM 2-deoxyglucose in the presence of lactose are reported. We found 2 classes of mutants following a series of experiments that included: growth rate determinations, uptake studies, measurement of phosphotransferase system (PTS) activities and detection of the IIIman proteins by Western blotting and analysis of [32P]PEP-phosphorylated proteins. Class 1 mutants did not possess the low-molecular-weight form of IIIman. They did not grow on mannose and were unable to transport 2-deoxyglucose. On the other hand, class 2 mutants possessed the 2 forms of IIIman, grew readily on mannose and transported 2-deoxyglucose, albeit at a lower rate than the parental strain. Both classes of mutants exhibited abnormal growth in media containing mixtures of sugars. Moreover, derepression of genes coding for catabolic enzymes was observed in all the mutant strains. Our data suggested that the role of the mannose PTS in the control of sugar utilization in S. salivarius is complex and may involve the participation of several components.

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Glycosylimidate, 8. Synthese von 1‐Thioglycosiden

1983

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Das reaktive acetylgeschutzte 0-(a-D-Glucopyranosy1)trichloracetimidat 3 liefert mit S-Nucleophilen unter Bortrifluorid-Ether-Katalyse erwartungsgemll3 unter Inversion der Konfiguration ausschliel3lich 1-Thio-P-D-glucopyranoside. Mit dern entsprechenden benzylgeschiitzten a-Trichloracetirnidat 4 werden unter Retention der Konfiguration ausschliel3lich 1-Thio-a-D-glucopyranoside erhalten. Die Bedeutung von Alkyl-1-thio-B-o-galactopyranosiden zur Enzyminduktion war AnlaR, diese einfache Glycosylubertragung auch zur Synthese von Isopropyl-1-thio-P-Dgalactopyranosid (12a) und des Natriumsalzes von 1-Thio-P-D-galactopyranose (12b) anzuwenden. Glycosylimidates, 8 I). -Synthesis of 1-ThioglycosidesAs expected, the reactive acetyl-protected O-(a-D-glUCOpyranOSy1) trichloroacetimidate 3 reacts with S-nucleophiles and trifluoroborane-ether as catalyst to yield exclusively I-thio-P-D-glucopyranosides with inversion of the configuration. The corresponding benzyl-protected a-trichloroacetimidate 4 affords with retention of the configuration alkyl 1 -thio-a-D-glucopyranosides. The importance of alkyl I-thio-f3-D-galactopyranosides in enzyme induction was reason to apply this convenient and efficient glycosyl-transfer reaction to the synthesis of isopropyl 1-thi0-D-Dgalactopyranoside (12a) and the sodium salt of 1-thio-P-D-galactopyranose (12b), respectively.

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Co-induction of beta-galactosidase and the lactose-P-enolpyruvate phosphotransferase system in Streptococcus salivarius and Streptococcus mutans

Cited by 61 publications

References 26 publications

Two functionally different glucose phosphotransferase transport systems in Streptococcus mutans and Streptococcus sobrinus

Two functionally different glucose phosphotransferase transport systems in Streptococcus mutans and Streptococcus sobrinus

Control of sugar utilization in oral streptococci. Properties of phenotypically distinct 2‐deoxyglucose‐resistant mutants of Streptococcus salivarius

Glycosylimidate, 8. Synthese von 1‐Thioglycosiden

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