Co-Incubation of Human Spermatozoa with Anti-VDAC Antibody Reduced Sperm Motility

Liu, Bianjiang; Tang, Min; Han, Ze‐Guang; Li, Jie; Zhang, Jiexiu; Lu, Pei-Hua; Song, Ninghong; Wang, Zengjun; Chen, Yin; Zhang, Wei

doi:10.1159/000356657

Cited by 9 publications

(7 citation statements)

References 36 publications

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“…In addition, velocity parameters (VSL, VAP and VCL) significantly decreased at pH 6.2 and 5.2, which was consistent with the result of motility. Additionally, fertilization success was always found to be correlated with VSL, VAP and VCL in the chicken, turkeys and human [ 31 – 33 ]. Our study showed that in acidic environments (pH 5.2 and 6.2), most of the sperm, which stayed within 1 cm from basal part of capillary pipettes, swam in situ , and few of them swam upward in the sperm penetration meter test.…”

Section: Discussionmentioning

confidence: 99%

The Semen pH Affects Sperm Motility and Capacitation

et al. 2015

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As the chemical environment of semen can have a profound effect on sperm quality, we examined the effect of pH on the motility, viability and capacitation of human sperm. The sperm in this study was collected from healthy males to avoid interference from other factors. The spermatozoa cultured in sperm nutrition solution at pH 5.2, 6.2, 7.2 and 8.2 were analyzed for sperm total motility, progressive motility (PR), hypo-osmotic swelling (HOS) rate, and sperm penetration. Our results showed that these parameters were similar in pH 7.2 and 8.2 sperm nutrition solutions, but decreased in pH 5.2 and 6.2 solutions. The HOS rate exhibited positive correlation with the sperm total motility and PR. In addition, the sperm Na+/K+-ATPase activity at different pHs was measured, and the enzyme activity was significantly lower in pH 5.2 and 6.2 media, comparing with that in pH 8.2 and pH 7.2 solutions. Using flow cytometry (FCM) and laser confocal scanning microscopy (LCSM) analysis, the intracellular Ca2+ concentrations of sperm cultured in sperm capacitation solution at pH 5.2, 6.2, 7.2 and 8.2 were determined. Compared with that at pH 7.2, the mean fluorescence intensity of sperm in pH 5.2 and 6.2 media decreased significantly, while that of pH 8.2 group showed no difference. Our results suggested that the declined Na+/K+-ATPase activity at acidic pHs result in decreased sperm movement and capacitation, which could be one of the mechanisms of male infertility.

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Section: Discussionmentioning

confidence: 99%

The Semen pH Affects Sperm Motility and Capacitation

et al. 2015

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“…Flagellar beating and swimming velocity is triggered and maintained by Ca influx [Carlson et al 2003;Liu et al 2014;Ren et al 2001;Yoshida et al 2003]. Plasma membrane calcium pumps depend on sufficient ATP [Cartwright and Neyses 2010] and we hypothesized that ATP deficiency might result in impaired [Ca 2+ ] i .…”

Section: Discussionmentioning

confidence: 99%

The role of mitochondrial aconitate (ACO2) in human sperm motility

Tang

Liu

Wang

et al. 2014

Systems Biology in Reproductive Medicine

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Deficiencies in tricarboxylic acid (TCA) cycle enzymes have been shown to cause a wide spectrum of human diseases, including malignancies and neurological and cardiac diseases. In mammalian spermatozoa mitochondria, the TCA cycle is known to be a crucial metabolic pathway that contributes to produce ATP. There is little known about the role and mechanism of mitochondrial aconitase (ACO2), which is an important regulatory enzyme of the TCA cycle, in asthenozoospermia. In the current study, immunofluorescence staining localized ACO2 to the human sperm mid-piece. By immunoblotting, we demonstrated that the level of ACO2 protein in asthenozoospermic samples was significantly decreased compared with that in normal fertile men. Importantly, we first observed that co-incubation of isocitrate with low motile sperm suspensions significantly improved sperm motility, which might be due to elevated intracellular ATP. The improvement of the sperm motility by isocitrate may have important clinical implications in the treatment of asthenozoospermia and certainly warrants further investigation.

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“…Absorbance was measured at 450 nm using a microtiter plate reader (Bio-Rad, USA). Cellular ATP levels were determined using an ATP Assay Kit (Beyotime Institute of Biotechnology, China) according to the manufacturer's protocol [16].…”

Section: Cmec Isolation Culture and In Vitro Iri Assaymentioning

confidence: 99%

Melatonin Attenuates Myocardial Ischemia/Reperfusion Injury by Inhibiting Autophagy Via an AMPK/mTOR Signaling Pathway

Chen

Liu

Chen

et al. 2018

Cell Physiol Biochem

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Background/Aims: Melatonin has been demonstrated to protect cardiac microvascular endothelial cells (CMECs) against ischemia/reperfusion injury (IRI). Autophagy plays different roles in the heart during ischemia and reperfusion. The AMP activated protein kinase/mammalian target of rapamycin (AMPK/mTOR) pathway is associated with autophagy. This study sought to explore whether melatonin regulates CMEC autophagy through the AMPK/mTOR signaling pathway. Methods: The effects of melatonin in IRI were investigated in vivo rat models and in vitro neonatal CMECs. Myocardial infarct size was achieved by Evans blue and triphenyltetrazolium chloride staining. The severity of cell injury was evaluated by cell vitality and lactate dehydrogenase (LDH) release assays, and autophagy was evaluated by transmission electron microscopy and the assessment of autophagy-related gene expression, such as that of Beclin 1 and light chain 3-II. Results: In vivo, melatonin markedly reduced infarcted area, improved cardiac function and decreased LDH release. However, the AMPK activator AICAR and the mTOR inhibitor rapamycin reduced the protective effects of melatonin on IRI. In vitro, Beclin1 and light chain 3-II protein were found to be down-regulated and autophagosomes were found to be reduced in response to melatonin, together with an increase in cell vitality and a decrease in LDH. Treatment with AICAR or rapamycin ablated the benefit observed with melatonin treatment. Conclusions: Melatonin played an important and protective role in CMECs by inhibiting autophagy against IRI via the AMPK/mTOR system.

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Co-Incubation of Human Spermatozoa with Anti-VDAC Antibody Reduced Sperm Motility

Cited by 9 publications

References 36 publications

The Semen pH Affects Sperm Motility and Capacitation

The Semen pH Affects Sperm Motility and Capacitation

The role of mitochondrial aconitate (ACO2) in human sperm motility

Melatonin Attenuates Myocardial Ischemia/Reperfusion Injury by Inhibiting Autophagy Via an AMPK/mTOR Signaling Pathway

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