Co-Incidence of Epstein–Barr Virus and High-Risk Human Papillomaviruses in Cervical Cancer of Syrian Women

Al-Thawadi, Hamda; Ghabreau, Lina; Aboulkassim, Tahar; Yasmeen, Amber; Vranić, Semir; Batist, Gerald; Moustafa, Ala‐Eddin Al

doi:10.3389/fonc.2018.00250

Cited by 31 publications

(72 citation statements)

References 34 publications

(44 reference statements)

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“…Our study provides evidence that HPV and EBV can be detected and quantified in blood samples of healthy blood donors without any obvious HPV infection, suggesting potential viral persistence at different anatomical sites. This random distribution of HPV subtypes along with EBV, suggests potential facilitative or competitive interactions among these onco-viruses, which has been recently reported by several studies [31,34,46,66]. Since these oncoviruses can be found in various cancers; our group has previously demonstrated that E6/E7 of high-risk HPVs can convert non-invasive cancer cells to invasive phenotypes [22].…”

Section: Resultssupporting

confidence: 61%

“…Amplification conditions included an initial heat activation step of 15 min at 95 °C; 40 cycles of PCR amplifications at 95 °C for 30 s, annealing (Table 1) for 30 s in addition to 1 min at 72 °C; and a final extension step of 72 °C for 10 min. Analysis was performed as previously described by our group [33,34]. Amplified products were analyzed by 1.5% agarose gel electrophoresis and visualized using iBrightCL1000 Imaging System (Ther-moFisher).…”

Section: Hpv and Ebv Detectionmentioning

confidence: 99%

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Co-prevalence of human Papillomaviruses (HPV) and Epstein–Barr virus (EBV) in healthy blood donors from diverse nationalities in Qatar

et al. 2020

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Background: Infections by both human oncoviruses, human Papillomaviruses (HPV) and Epstein-Barr virus (EBV) are very common in the adult human population and are associated with various malignancies. While HPV is generally transmitted sexually or via skin-to-skin contact, EBV is frequently transmitted by oral secretions, blood transfusions and organ transplants. This study aims to determine the prevalence and circulating genotypes of HPV and EBV in healthy blood donors in Qatar. Methods: We explored the co-prevalence of high-risk HPVs and EBV in 378 males and only 7 females blood donors of different nationalities (mainly from Qatar, Egypt, Syria, Jordan, Pakistan, and India) residing in Qatar, using polymerase chain reaction (PCR). DNA was extracted from the buffy coat and genotyping was performed using PCR and nested-PCR targeting E6 and E7 as well as LMP-1 of HPV and EBV, respectively. Results: We found that from the total number of 385 cases of healthy blood donors studied, 54.8% and 61% of the samples are HPVs and EBV positive, respectively. Additionally, our data revealed that the co-presence of both high-risk HPVs and EBV is 40.4% of the total samples. More significantly, this study pointed out for the first time that the most frequent high-risk HPV types in Qatar are 59 (54.8%), 31 (53.7%), 52 (49.1%), 51 (48.6%), 58 (47%) and 35 (45.5%), while the most commonly expressed low-risk HPV types are 53 (50.6%), 11 (45.5), 73 (41.7%) and 6 (41.3%), with all the cases showing multiple HPVs infection. Conclusion: In this study, we demonstrated for the first time that HPV and EBV are commonly co-present in healthy blood donors in Qatar. On the other hand, it is important to highlight that these oncoviruses can also be co-present in several types of human cancers where they can cooperate in the initiation and/or progression of these cancers. Therefore, more studies regarding the co-presence of these oncoviruses and their interaction are necessary to understand their cooperative role in human diseases.

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Section: Resultssupporting

confidence: 61%

Section: Hpv and Ebv Detectionmentioning

confidence: 99%

Co-prevalence of human Papillomaviruses (HPV) and Epstein–Barr virus (EBV) in healthy blood donors from diverse nationalities in Qatar

et al. 2020

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“…Recent research suggests that a coinfection of EBV and HPV may play an important role in the progression of cervical cancer [28]. The study found that EBV and high-risk HPV were copresent in 34% of the 44 cervical cancer tissues sampled, and the cancers with both infections were likely to be more aggressive [28].…”

Section: Oncoviruses and Breast Cancermentioning

confidence: 86%

“…Another study used PCR and tissue microarray (TMA) analysis to show that EBV was present in 36% of 102 CRC tissue samples, and EBV was also associated with a more aggressive type of CRC [27]. Recent research suggests that a coinfection of EBV and HPV may play an important role in the progression of cervical cancer [28]. The study found that EBV and high-risk HPV were copresent in 34% of the 44 cervical cancer tissues sampled, and the cancers with both infections were likely to be more aggressive [28].…”

Section: Oncoviruses and Breast Cancermentioning

confidence: 99%

How latent viruses cause breast cancer: An explanation based on the microcompetition model

Polansky

Schwab

2018

Bosn J of Basic Med Sci

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Most breast cancer cases show a decrease in the concentration of the breast cancer type 1 susceptibility protein (BRCA1). However, only a small portion of these cases have a mutated BRCA1 gene. Although many attempts have been made to identify the reason for the decrease in BRCA1 concentration in sporadic, non-heritable breast cancer cases, the cause is still unknown. In this review, we use the Microcompetition Model to explain how certain latent viruses, which are frequently detected in breast cancer tumors, can decrease the expression of the BRCA1 gene and cause the development of breast tumors.

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“…8 EBV causes infectious mononucleosis (benign, self-limited disease) and several lymphoproliferative and epithelial malignancies including B-cell lymphomas (Burkitt lymphoma, Hodgkin lymphoma, post-transplant lymphoproliferative disorder), T-cell/NK lymphomas, nasopharyngeal and gastric carcinomas. [11][12][13] In addition, EBV expression has also been demonstrated in several other carcinomas such as breast, prostate, oral, cervical and salivary gland carcinomas; [14][15][16][17][18] Nevertheless no causal relationship has been established yet.…”

Section: Introductionmentioning

confidence: 99%

Epstein-Barr virus (EBV) status in colorectal cancer: a mini review

Bedri

Sultan

Alkhalaf

et al. 2018

Human Vaccines & Immunotherapeutics

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Epstein-Barr virus (EBV) is a well-characterized oncovirus, associated with several malignancies. The complex and heterogeneous nature of colorectal cancer (CRC) has led to many epidemiological causal associations with CRC. However, a direct causal link between microbial infections and CRC has not been established yet. Our review indicates that the current evidence for the presence and role in EBV in CRC is insufficient and contradictory. The design of the analyzed studies, sample size as well as methodology used for EBV detection varied markedly and consequently may not lead to meaningful conclusions. The presence of EBV in other colorectal tumors (lymphomas, smooth muscle tumors) is in line with their status at other anatomic locations and may have therapeutic implications with EBV-specific vaccines. On the other hand, studies exploring EBV in colorectal adenoma-carcinoma sequence and its molecular genetic characteristics are largely missing and may significantly contribute to a better understanding of the role of EBV in CRC.

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Co-Incidence of Epstein–Barr Virus and High-Risk Human Papillomaviruses in Cervical Cancer of Syrian Women

Cited by 31 publications

References 34 publications

Co-prevalence of human Papillomaviruses (HPV) and Epstein–Barr virus (EBV) in healthy blood donors from diverse nationalities in Qatar

Co-prevalence of human Papillomaviruses (HPV) and Epstein–Barr virus (EBV) in healthy blood donors from diverse nationalities in Qatar

How latent viruses cause breast cancer: An explanation based on the microcompetition model

Epstein-Barr virus (EBV) status in colorectal cancer: a mini review

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