Co-immobilized β-galactosidase and Saccharomyces cerevisiae cells for the simultaneous synthesis and purification of galacto-oligosaccharides

One-pot biotransformation of glycerol into serinol catalysed by biocatalytic composites made of whole cells and immobilised enzymes

“…For both glycerol qualities, the production of both serinol and DHA diminishes to less than 40% and 10% of its initial capacity after the second and third 23 h cycles, respectively. We suggest that this poor robustness relies on the low stability of Pf-ATA@AG-Co 2+ as supported by previous results that report the dramatic inactivation of this enzyme during its sequential use for the synthesis of aminoalcohols 20 . Furthermore, we remark that the biocatalyst integrity is affected by the components of crude glycerol, as the alginate matrix completely disintegrates after 3 cycles.…”

“…Since the entrapment of the free enzyme might cause the protein diffusion to the bulk owing to the large pores of the hydrogel macroparticles, we co-entrapped G. oxydans resting cells and Pf-ATA@AG-Co 2+ into alginate pearls to avoid the enzyme lixiviation. A similar approach was used by Wilson et al to entrap cross-linked enzyme aggregates into alginate gel particles for the simultaneous synthesis and purification of galacto-oligosaccharides from lactose 20 .…”

A Novel Thermal-Activated β-Galactosidase from Bacillus aryabhattai GEL-09 for Lactose Hydrolysis in Milk

“…Even though several β-galactosidases have been heterologously expressed and identified, only a few are available for commercial applications [53]. Currently, the food industry utilizes β-galactosidase from Saccharomyces cerevisiae and Aspergillus oryzae [54], both of which have relatively good properties, including an optimum pH for breaking down lactose in milk (6.0-7.0). lacZBa has the best activity at pH 6.0 and is stable at pH values between 6.0 and 7.0, which is compatible with the application of lacZBa in dairy products.…”

Trends in lactose-derived bioactives: synthesis and purification

“…Typically, the process is conducted in such a way as to promote the transformation of unwanted carbohydrates into ethanol and carbon dioxide, which are easier to remove from the medium [154][155][156]. Thus, selective fermentation stands out as a simple and low-cost method for attaining high purity prebiotic preparations [38,88,160]. However, high biomass concentration is generally required for the effective removal of the contaminant sugars, and the generation of metabolites, like ethanol, glycerol, higher alcohols and organic acid, might make necessary to include additional purification steps to properly remove them [44,160].…”

“…Other authors have reported the use of this strategy not only to increase the purity of the reacted mixtures but also to decrease the enzyme inhibition caused by monosaccharides. Aburto et al [160] evaluated the synthesis of GOS and its simultaneous purification in one-pot mode using a biocatalyst made by entrapping S. cerevisiae cells and crosslinked aggregates of A. oryzae β-galactosidases in alginate gels. The purpose was to assess if the continuous removal of the monosaccharides increased the yield and productivity of synthesis, as well as the purity of the product, but, contrary to expected, slightly lower reaction yields and purities were obtained than in conventional batch synthesis of GOS.…”