Co-immobilized bienzyme of horseradish peroxidase and glucose oxidase on dopamine-modified cellulose–chitosan composite beads as a high-efficiency biocatalyst for degradation of acridine

Gu, Yaohua; Yuan, Lin; Li, Mingming; Wang, Xinyu; Rao, Deyu; Bai, Xiaoyan; Shi, Keren; Xu, Haiming; Hou, Shaozhang; Yao, Huiqin

doi:10.1039/d2ra04091c

Cited by 16 publications

(6 citation statements)

References 44 publications

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“…Additionally, after 30 days of storage, the activity of free HRP and Mb dropped to 30% of their initial activity, while the activity of immobilized HRP and Mb was greater than 65% after 30 days. These results indicated that immobilization provided a relatively stable microenvironment for HRP and Mb molecules, making them less prone to inactivation during storage [31,49]. This finding is consistent with prior research indicating that calcium alginate does not react with the by-product and can effectively prolong the enzyme's durability, hence resulting in enhanced removal efficiency [50].…”

Section: Stability Of Immobilized Enzymesupporting

confidence: 88%

“…Water 2024, 16, x FOR PEER REVIEW 8 of 13 immobilization provided a relatively stable microenvironment for HRP and Mb molecules, making them less prone to inactivation during storage [31,49]. This finding is consistent with prior research indicating that calcium alginate does not react with the by-product and can effectively prolong the enzyme's durability, hence resulting in enhanced removal efficiency [50].…”

Section: Single Factor Explorationsupporting

confidence: 83%

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Immobilization of Horseradish Peroxidase and Myoglobin Using Sodium Alginate for Treating Organic Pollutants

Wang,

Ghanizadeh,

Khan

et al. 2024

Water

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Removing organic pollutants from wastewater is crucial to prevent environmental contamination and protect human health. Immobilized enzymes are increasingly being explored for wastewater treatment due to their specific catalytic activities, reusability, and stability under various environmental conditions. Peroxidases, such as horseradish peroxidase (HRP) and myoglobin (Mb), are promising candidates for immobilized enzymes utilized in wastewater treatment due to their ability to facilitate the oxidation process of a wide range of organic molecules. However, the properties of the carrier and support materials greatly influence the stability and activity of immobilized HRP and Mb. In this research, we developed immobilized HRP and Mb using support material composed of sodium alginate and CaCl2 as carriers and glutaraldehyde as a crosslinking agent. Following this, the efficacy of immobilized HRP and Mb in removing aniline, phenol, and p-nitrophenol was assessed. Both immobilized enzymes removed all three organic pollutants from an aqueous solution, but Mb was more effective than HRP. After being immobilized, both enzymes became more resilient to changes in temperature and pH. Both immobilized enzymes retained their ability to eliminate organic pollutants through eight treatment cycles. Our study uncovered novel immobilized enzyme microspheres and demonstrated their successful application in wastewater treatment, paving the way for future research.

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Section: Stability Of Immobilized Enzymesupporting

confidence: 88%

Section: Single Factor Explorationsupporting

confidence: 83%

Immobilization of Horseradish Peroxidase and Myoglobin Using Sodium Alginate for Treating Organic Pollutants

Wang,

Ghanizadeh,

Khan

et al. 2024

Water

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“…The combination of GOx and HRP has been widely used either to develop glucose biosensors or to elaborate biofuel cells [ 17 , 18 ]. In this work, the enzyme cascade model involved the sequential reactions of two enzymes as well as a mediated electrical connection step of one enzyme.…”

Section: Resultsmentioning

confidence: 99%

A Conductive Microcavity Created by Assembly of Carbon Nanotube Buckypapers for Developing Electrochemically Wired Enzyme Cascades

Jeerapan,

Nedellec,

Cosnier

2024

Nanomaterials

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We describe the creation of a conductive microcavity based on the assembly of two pieces of carbon nanotube buckypaper for the entrapment of two enzymes, horseradish peroxidase (HRP) and glucose oxidase (GOx), as well as a redox mediator: 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid diammonium salt (ABTS). The hollow electrode, employing GOx, HRP, and the mediator, as an electrochemical enzyme cascade model, is utilized for glucose sensing at a potential of 50 mV vs. Ag/AgCl. This bienzyme electrode demonstrates the ability to oxidize glucose by GOx and subsequently convert H2O2 to water via the electrical wiring of HRP by ABTS. Different redox mediators (ABTS, potassium hexacyanoferrate (III), and hydroquinone) are tested for HRP wiring, with ABTS being the best candidate for the electroenzymatic reduction of H2O2. To demonstrate the possibility to optimize the enzyme cascade configuration, the enzyme ratio is studied with 1 mg HRP combined with variable amounts of GOx (1–4 mg) and 2 mg GOx combined with variable amounts of HRP (0.5–2 mg). The bienzyme electrode shows continuous operational stability for over a week and an excellent storage stability in phosphate buffer, with a decay of catalytic current by only 29% for 1 mM glucose after 100 days.

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“…First, 0.1 g ZnFe 2 O 4 nanoparticles and 1.0 g PEG-400 were dispersed in 50 mL Tris-HCl buffer (50 mmol•L −1 , pH 8.5), stirred well, and sonicated for 30 min. After the ultrasound, 0.1 g of dopamine (DA) was slowly added to the suspension and mechanically stirred at 300 r•min −1 for 3 h. By selfpolymerizing dopamine in alkaline conditions, nanoparticles coated with polydopamine (PDA) were formed [22]. After the reaction, the separated ZnFe 2 O 4 @PDA nanoparticles were washed with deionized water.…”

Section: Synthesis Of Znfe 2 O 4 @Pdamentioning

confidence: 99%

Facile Preparation of Dopamine-Modified Magnetic Zinc Ferrite Immobilized Lipase for Highly Efficient Synthesis of OPO Functional Lipid

Xue¹,

Chen²,

Yang³

et al. 2023

Journal of Renewable Materials

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1,3-Dioleoyl-2-palmitoylglycerol (OPO) has been a hotspot of functional oils research in recent years, but due to the high cost of sn-1,3 specific lipase in enzymatic synthesis and the lack of biocatalyst stability, large-scale industrial application is difficult. In this study, the prepared magnetic ZnFe 2 O 4 was functionalized with dopamine to obtain ZnFe 2 O 4 @PDA, and the nano-biocatalyst ZnFe 2 O 4 @PDA@RML was prepared by immobilizing sn-1,3 specific lipase of Rhizomucor miehei lipase (RML) via a cross-linking method. The existence of RML on ZnFe 2 O 4 @P-DA was confirmed by XRD, FTIR, SEM, and TEM. This strategy proved to be simple and effective because the lipase immobilized on magnetic nanoparticles could be quickly recovered using external magnets, enabling reuse of the lipase. The activity, adaptability to a high temperature, pH value, and operational stability of immobilized RML were superior to those of free RML. After optimizing the synthesis conditions, the OPO yield was 42.78%, and the proportion of PA at the sn-2 position (PA-Sn2) was 54.63%. After the first four cycles, the activity of ZnFe 2 O 4 @PDA@RML was not significantly affected. The magnetically immobilized lipase has good thermal stability, long-term storage stability, reusability, and high catalytic activity. It can be used as a green and efficient biocatalyst to synthesize the OPO functional lipid.

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Co-immobilized bienzyme of horseradish peroxidase and glucose oxidase on dopamine-modified cellulose–chitosan composite beads as a high-efficiency biocatalyst for degradation of acridine

Abstract: Co-immobilized bienzyme biocatalysts are attracting increasing interest in the field of wastewater treatment due to their widespread application.

Cited by 16 publications

References 44 publications

Immobilization of Horseradish Peroxidase and Myoglobin Using Sodium Alginate for Treating Organic Pollutants

Immobilization of Horseradish Peroxidase and Myoglobin Using Sodium Alginate for Treating Organic Pollutants

A Conductive Microcavity Created by Assembly of Carbon Nanotube Buckypapers for Developing Electrochemically Wired Enzyme Cascades

Facile Preparation of Dopamine-Modified Magnetic Zinc Ferrite Immobilized Lipase for Highly Efficient Synthesis of OPO Functional Lipid

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