Co-immobilization of Laccase and TEMPO onto Glycidyloxypropyl Functionalized Fibrous Phosphosilicate Nanoparticles for Fixing CO2 into β-Oxopropylcarbamatesin

Novel Bionanocompounds: Outer Membrane Protein A and Laccase Co-Immobilized on Magnetite Nanoparticles for Produced Water Treatment

“…Co-immobilization (MNP-PEA-OmpA-Laccase) led to a second weight loss of 5.6%, which shows a subtle improvement in efficiency. This could be attributed to the interplay and competition of protein-protein and protein-surface interactions during immobilization [ 69 , 70 , 71 , 72 ]. Further studies will be required to maximize the efficiencies.…”

“…For this purpose, after immobilization the activity of excess laccase in the medium was measured colorimetrically by monitoring the conversion of ABTS with respect to a calibration curve. An estimate of immobilization yield was calculated according to Equation (1) by comparing the activity of initially added laccase with that of the laccase remaining after immobilization, where U remaining Laccase is the catalytic activity unit contained in the recovered immobilization medium and U added Laccase is the unit initially added for immobilization according to the calculations reported in Supplementary Materials, Section SI2 [ 70 , 72 ]. ((U remaining Laccase)/(U added Laccase)) × 100.…”

Exovinylene Cyclic Carbonates: Multifaceted CO2‐Based Building Blocks for Modern Chemistry and Polymer Science

“…When secondary amines replace primary ones in multicomponent reactions, oxo‐carbamates with structural analogy to those obtained by direct aminolysis of preformed αCCs are synthesized. Various metal complexes ([RuCl 2 (norbornadiene)] n , [102] ([Cu(MeCN) 4 ]PF 6 )), [103] cooperative systems (Ag 2 CO 3 /PPh 3 [58] or ( p ‐MeOC 6 H 4 ) 3 P, [104] AgCl/Et 4 NCl [105] ), heterogeneous silver/copper‐based catalysts (CNT‐NHC‐Ag/Cu), [74] and even immobilized metallo‐enzyme (laccase) used in synergy with TEMPO [106] illustrate the large diversity of catalytic options that have been engineered to fabricate the carbamate scaffolds. As a trend, Ru and Cu catalysts require demanding operative conditions (40–50 bar, 70–100 °C) to deliver the envisioned products with high yields.…”

Synthesis and characterization of a novel TEMPO@FeNi3/DFNS–laccase magnetic nanocomposite for the reduction of nitro compounds

“…Aer that, 22 mmol of (3-glycidyloxypropyl)trimethoxysilane were added into the mixture at room temperature and stirred for 16 h at 50 C. The resultant mixture was ltered and washed with ethanol and deionized water. The ltered solids were then dried in vacuum condition at 50 C for 3 h. 28 General procedure for the preparation of TEMPO@FeNi 3 / DFNS-laccase MNPs Glycidyloxypropyl were used for the activation of FeNi 3 /DFNS (100 mg). This was then added to a mixture containing 10 mL of acetate buffer at 0.1 M and pH of 4.5, laccase and 4-hydroxy-TEMPO.…”

“…The resultant nanoparticles containing co-immobilized laccase and TEMPO were ltered and rinsed with acetate buffer multiple times. The ltered nanoparticles were kept and store under 4 C. 28 General procedure for reducing nitro compounds Suspension of NaBH 4 (1.5 mmol) in H 2 O (5 mL) was added to nitro compound (1 mmol) and stirring was applied at 50 C in the presence of TEMPO@FeNi 3 /DFNS-laccase (15 mg) in water. TLC was applied for monitoring the reaction progress.…”