Co-immobilization of cellobiose dehydrogenase and deoxyribonuclease I on chitosan nanoparticles against fungal/bacterial polymicrobial biofilms targeting both biofilm matrix and microorganisms

Tan, Yulong; Ma, Su; Leonhard, Matthias; Moser, Doris; Ludwig, Roland; Schneider‐Stickler, Berit

doi:10.1016/j.msec.2019.110499

Cited by 48 publications

(37 citation statements)

References 57 publications

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“…Very few authors compared the effect of nanosystems vectorizing antimicrobial agents on monoor multispecies biofilms [46,47], and even less on biofilms mixing Gram-positive bacteria and fungi. It is now established that the efficacy of nanosystems on biofilms is linked to their capacity for deeply penetrating the matrix [32].…”

Section: Discussionmentioning

confidence: 99%

“…In the study of Anjum et al, penetration was made easier by adding a ligand onto the nanoparticle surface targeting the biofilm matrix. Tan et al measured the antibiofilm activity of nanoparticles including enzymes targeting the matrix of biofilms composed of S. aureus and C. albicans [47]. The particles were able to disrupt in a similar manner single-species or dual-species biofilms, but it was observed that adhesion of bacteria to Candida hyphae made their surface less accessible to antimicrobial molecules.…”

Section: Discussionmentioning

confidence: 99%

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Nanovectorized Microalgal Extracts to Fight Candida albicans and Cutibacterium acnes Biofilms: Impact of Dual-Species Conditions

et al. 2020

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Biofilm-related infections are a matter of concern especially because of the poor susceptibility of microorganisms to conventional antimicrobial agents. Innovative approaches are needed. The antibiofilm activity of extracts of cyanobacteria Arthrospira platensis, rich in free fatty acids, as well as of extract-loaded copper alginate-based nanocarriers, were studied on single- and dual-species biofilms of Candida albicans and Cutibacterium acnes. Their ability to inhibit the biofilm formation and to eradicate 24 h old biofilms was investigated. Concentrations of each species were evaluated using flow cytometry. Extracts prevented the growth of C. acnes single-species biofilms (inhibition > 75% at 0.2 mg/mL) but failed to inhibit preformed biofilms. Nanovectorised extracts reduced the growth of single-species C. albicans biofilms (inhibition > 43% at 0.2 mg/mL) while free extracts were weakly or not active. Nanovectorised extracts also inhibited preformed C. albicans biofilms by 55% to 77%, whereas the corresponding free extracts were not active. In conclusion, even if the studied nanocarrier systems displayed promising activity, especially against C. albicans, their efficacy against dual-species biofilms was limited. This study highlighted that working in such polymicrobial conditions can give a more objective view of the relevance of antibiofilm strategies by taking into account interspecies interactions that can offer additional protection to microbes.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Nanovectorized Microalgal Extracts to Fight Candida albicans and Cutibacterium acnes Biofilms: Impact of Dual-Species Conditions

et al. 2020

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“…More recently, Tan et al co-immobilized DNase I and cellobiose dehydrogenase in chitosan NPs to treat monomicrobial and polymicrobial biofilms of Candida albicans and S. aureus. Cellobiose dehydrogenase was selected as an antimicrobial agent since it uses cello-oligomers as a substrate to produce hydrogen peroxidase, which generates free radicals that promote oxidation of biofilm matrix components and has bactericidal effects (Henriksson et al, 2000;Gao et al, 2014;Tan et al, 2020). The efficiency of the developed NPs was tested in 24 h-old biofilms.…”

Section: Nanocarriersmentioning

confidence: 99%

“…The efficiency of the developed NPs was tested in 24 h-old biofilms. The NPs revealed a high activity by reducing biofilm percentage more than 80% on both mono-and polymicrobial biofilms (Tan et al, 2020). NPs immobilizing only DNase I were also tested and showed no significant effect on the biofilms.…”

Section: Nanocarriersmentioning

confidence: 99%

“…According to the authors, this result is a consequence of the absence of a bactericidal agent, which allows dispersed bacterial cells to form a new biofilm. However, the co-immobilization with cellobiose dehydrogenase indicates a synergistic effect with DNase I (Tan et al, 2020). The enzyme alginate lyase was also immobilized in polymeric NPs for matrix disruption purposes.…”

Section: Nanocarriersmentioning

confidence: 99%

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Innovative Strategies Toward the Disassembly of the EPS Matrix in Bacterial Biofilms

et al. 2020

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Bacterial biofilms represent a major concern at a worldwide level due to the high demand for implantable medical devices and the rising numbers of bacterial resistance. The complex structure of the extracellular polymeric substances (EPS) matrix plays a major role in this phenomenon, since it protects bacteria from antibiotics, avoiding drug penetration at bactericidal concentrations. Besides, this structure promotes bacterial cells to adopt a dormant lifestyle, becoming less susceptible to antibacterial agents. Currently, the available treatment for biofilm-related infections consists in the administration of conventional antibiotics at high doses for a long-term period. However, this treatment lacks efficiency against mature biofilms and for implant-associated biofilms it may be necessary to remove the medical device. Thus, biofilm-related infections represent an economical burden for the healthcare systems. New strategies focusing on the matrix are being highlighted as alternative therapies to eradicate biofilms. Here, we outline reported matrix disruptive agents, nanocarriers, and technologies, such as application of magnetic fields, photodynamic therapy, and ultrasounds, that have been under investigation to disrupt the EPS matrix of clinically relevant bacterial biofilms. In an ideal therapy, a synergistic effect between antibiotics and the explored innovated strategies is aimed to completely eradicate biofilms and avoid antimicrobial resistance phenomena.

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Aggregation‐induced emission artificial enzyme (AIEzyme) with DNase‐like activity: Imaging and matrix cleavage for combating bacterial biofilm

et al. 2023

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DNase‐catalyzed hydrolysis of extracellular DNA (eDNA) has been widely employed to eradicate intractable biofilms. Although aggregation‐induced emission (AIE) has become the ideal tool for killing planktonic bacteria, AIE luminogens (AIEgens) often lack DNase‐mimetic activity, so as to suffer from poor anti‐biofilm capacity. Here, an “AIEzyme”, a kind of AIE nanomaterial with enzyme‐like activity, is designed and synthesized, where the AIEgens are used as the ligands of Zr‐based coordination polymer nanoparticles. Not only does AIEzyme have enduring DNase‐mimetic activity with low activation energy, but also structural rigidity‐stabilized fluorescence. Due to the long‐acting hydrolysis for eDNA in biofilm, AIEzyme can efficiently disorganize the established biofilms with good penetrability and realize the healing of superbug‐infected wounds under only one dose of AIEzyme. This work provides a strategy to endow ordinary AIEgens with DNase‐like and anti‐biofilm activities. Moreover, AIEzymes can be observed by virtue of their own AIE character, facilitating the study on self‐positioning and residual amount of AIEzymes in wounds. The concept “AIEzyme” would hopefully stimulate the tremendous expansion for the tool kits and the application of AIEgens and artificial enzymes.

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Co-immobilization of cellobiose dehydrogenase and deoxyribonuclease I on chitosan nanoparticles against fungal/bacterial polymicrobial biofilms targeting both biofilm matrix and microorganisms

Cited by 48 publications

References 57 publications

Nanovectorized Microalgal Extracts to Fight Candida albicans and Cutibacterium acnes Biofilms: Impact of Dual-Species Conditions

Nanovectorized Microalgal Extracts to Fight Candida albicans and Cutibacterium acnes Biofilms: Impact of Dual-Species Conditions

Innovative Strategies Toward the Disassembly of the EPS Matrix in Bacterial Biofilms

Aggregation‐induced emission artificial enzyme (AIEzyme) with DNase‐like activity: Imaging and matrix cleavage for combating bacterial biofilm

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