Co-expression of radial glial marker in macrophages/microglia in rat spinal cord contusion injury model

Eicosapentaenoic Acid Prevents Memory Impairment After Ischemia by Inhibiting Inflammatory Response and Oxidative Damage

“…20 After blocking of nonspecific binding with 1% albumin solution and washing in 0.01 M PBS, a monoclonal antibody against Iba1 (1:100 dilution; Wako, Osaka, Japan) was applied to sections overnight at 4 C. Sections were incubated with biotinylated goat anti-rabbit IgG (1:1000 dilution; Vector Laboratories, Burlingame, CA) for 90 minutes and then incubated with avidin-biotin horseradish peroxidase (Vector Laboratories) for 90 minutes. 21 A molecular histochemical system (Apoptosis In Situ Detection Kit; Wako) was used for specific staining of DNA fragmentation (ie, TUNEL staining). 22 This method is based on the specific binding of terminal deoxynucleotidyl transferase (TdT) to the 3 0 -OH ends of DNA.…”

Glial and axonal regeneration following spinal cord injury

“…These findings provide evidence that at a certain time after SCI, microglia and radial glia can be demonstrated to share the same cell lineage. 58 Studies conducted so far have shown that microglia activation after SCI does not follow a fixed pattern with respect to the sites of detection and the time course. 59 Microglia et al 45 also observed that the spherical cells produced during division of radial glia soon give rise to projections and become bipolar cells.…”

Cell-replacement therapy and neural repair in the retina

“…Following ON transection, the microglial fraction expressing nestin proliferates in situ and we therefore conclude that microglial nestin expression probably plays a role in the physiological self-renewal of this endogenous cell population (Wohl et al 2010;Wohl et al 2011). Since increasing evidence suggests that CNS injury can induce neural progenitor characteristics in activated microglia of non-neurogenic regions in vivo (Wu et al 2005;Yokoyama et al 2006), we propose that this specific retinal microglial subpopulation acts as an endogenous neural progenitor-like cell after injury. This latter issue, however, deserves further research to evaluate the potential of retinal microglia as a source for cell replacement to treat retinal degenerative diseases.…”