Co-Expression of Monodehydroascorbate Reductase and Dehydroascorbate Reductase from Brassica rapa Effectively Confers Tolerance to Freezing-Induced Oxidative Stress

Shin, Sun‐Young; Kim, Myung‐Hee; Kim, Yul-Ho; Park, Hyang-Mi; Yoon, Ho-Sung

doi:10.1007/s10059-013-0071-4

Cited by 46 publications

(22 citation statements)

References 62 publications

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“…Under salt stress, transgenic plum plantlet with SOD expressed non-enzymatic antioxidants, glutathione and ascorbate which lowered hydrogen peroxide level (Diaz-Vivancos et al 2013). In our study, it is likely that upregulation of SOD and a peroxidase is involved in scavenging ROS.Another protein, monodehydroascorbate reductase (MDHAR) which was upregulated only in B group is essential for regenerating ascorbate during ROS scavenging activity as evidenced by transgenic Brassica rapa overexpressing MDHAR and dehydroascorbate reductase (DHAR) increased glutathione and phenolics thereby alleviating redox stress leading to cold tolerance(Shin et al 2013).…”

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confidence: 75%

Proteomics provides insights into biological pathways altered by plant growth promoting bacteria and arbuscular mycorrhiza in sorghum grown in marginal soil

Dhawi

Datta

Ramakrishna

2017

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

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Sorghum is an economically important crop, a model system for gene discovery and a biofuel source. Sorghum seedlings were subjected to three microbial treatments, plant growth promoting bacteria (B), arbuscular mycorrhizal (AM) fungi mix with two Glomus species (G. aggregatum and G. etunicatum), Funelliformis mosseae and Rhizophagus irregularis (My), and B and My combined (My+B). Proteomic analysis was conducted followed by integration with metabolite, plant biomass and nutrient data. Out of 366 differentially expressed proteins in sorghum roots, 44 upregulated proteins overlapping among three treatment groups showed positive correlation with sorghum biomass or element uptake or both. Proteins upregulated only in B group include asparagine synthetase which showed negative correlation with biomass and uptake of elements. Phosphoribosyl amino imidazole succinocarboxamide protein with more than 50-fold change in My and My+B groups correlated positively with Ca, Cu, S and sucrose levels in roots. The B group showed the highest number of upregulated proteins among the three groups with negative correlation with sorghum biomass and element uptake. KEGG pathway analysis identified carbon fixation as the unique pathway associated with common upregulated proteins while biosynthesis of amino acids and fatty acid degradation were associated with common downregulated proteins. Protein-protein interaction analysis using STRING identified a major network with thirteen downregulated proteins. These findings suggest that plant-growth-promoting-bacteria alone or in combination with mycorrhiza enhanced radical scavenging system and increased levels of specific proteins thereby shifting the metabolism towards synthesis of carbohydrates resulting in sorghum biomass increase and uptake of nutrients.

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confidence: 75%

Proteomics provides insights into biological pathways altered by plant growth promoting bacteria and arbuscular mycorrhiza in sorghum grown in marginal soil

Dhawi

Datta

Ramakrishna

2017

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

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“…Previous studies have shown that multiple ion channel pores in cell participate to stress responses in cyanobacterial cells (Los and Murata, 2004;Kim et al, 2018). SDS and cold stress were a surfactant that damage lipid membranes, thereby disrupting cellular redox homeostasis and leading to apoptosis (Kwon et al, 2003;Tahara et al, 2012;Shin et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Expression of Heterologous OsDHAR Gene Improves Glutathione (GSH)-Dependent Antioxidant System and Maintenance of Cellular Redox Status in Synechococcus elongatus PCC 7942

Kim¹,

Park²,

Kim³

et al. 2020

Front. Plant Sci.

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An excess of reactive oxygen species (ROS) can cause severe oxidative damage to cellular components in photosynthetic cells. Antioxidant systems, such as the glutathione (GSH) pools, regulate redox status in cells to guard against such damage. Dehydroascorbate reductase (DHAR, EC 1.8.5.1) catalyzes the glutathione-dependent reduction of oxidized ascorbate (dehydroascorbate) and contains a redox active site and glutathione binding-site. The DHAR gene is important in biological and abiotic stress responses involving reduction of the oxidative damage caused by ROS. In this study, transgenic Synechococcus elongatus PCC 7942 (TA) was constructed by cloning the Oryza sativa L. japonica DHAR (OsDHAR) gene controlled by an isopropyl β-D-1thiogalactopyranoside (IPTG)-inducible promoter (Ptrc) into the cyanobacterium to study the functional activities of OsDHAR under oxidative stress caused by hydrogen peroxide exposure. OsDHAR expression increased the growth of S. elongatus PCC 7942 under oxidative stress by reducing the levels of hydroperoxides and malondialdehyde (MDA) and mitigating the loss of chlorophyll. DHAR and glutathione S-transferase activity were higher than in the wild-type S. elongatus PCC 7942 (WT). Additionally, overexpression of OsDHAR in S. elongatus PCC 7942 greatly increased the glutathione (GSH)/glutathione disulfide (GSSG) ratio in the presence or absence of hydrogen peroxide. These results strongly suggest that DHAR attenuates deleterious oxidative effects via the glutathione (GSH)-dependent antioxidant system in cyanobacterial cells. The expression of heterologous OsDHAR in S. elongatus PCC 7942 protected cells from oxidative damage through a GSH-dependent antioxidant system via GSH-dependent reactions at the redox active site and GSH binding site residues during oxidative stress.

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“…MDAR is an enzyme that catalyzes the conversion of monodehydroascorbate in ascorbate [NADH + H + + 2 monodehydroascorbate → NAD + + 2 ascorbate]. MDHAR activation in response to various stress conditions has been observed in many plant species (Shin et al, 2013). Ascorbate is a very important antioxidant molecule in plants for the maintenance of ROS scavenging ability.…”

Section: Discussionmentioning

confidence: 99%

Proteome of germinating and dormant axillary buds of sugarcane and analysis of sprouting-related proteins

Benez

Maranho

et al. 2019

Plant Omics

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The germination potential of the axillary buds is crucial for the installation of new sugarcane crop and is highly relevant for the maintenance of the crop during the regrowth cycles. In this way, 1-DE-UPLC-ESI-Q-TOF system of mass spectrometry in a shotgun approach evaluated total and differential proteome of germinating and dormant axillary buds of cultivar RB867515 in second ratoon. The current paper investigates whether differently expressed proteins can be detected in germinating axillary buds or not. The proteome of germinative and dormant axillary buds were obtained using TCA/acetone in triplicates with subsequent proteins and polypeptides pre-separation by SDS-PAGE and UPLC. Mass spectra were obtained via MS/MS and were confronted with Viridiplante mass spectra from NCBIprot, SwissProt and UniProtKB databases, using Mascot tool from Matrix Science. A total of 159 proteins were identified. In total, 46 proteins were exclusively detected in the germinating buds, while 41 proteins were exclusively reported in the dormant buds and 72 proteins were common in germinating and dormant buds. Different proteins, involved with biotic and abiotic stress, were detected at equivalent proportions in germinating (29.5%) and dormant (31.7%) buds, while contrasting proportions of proteins involved with energy metabolism were detected in germinating (22.7%) and dormant (12%) buds. Budding potential in the third cut seems to be determined by specific proteins related to energy metabolism and steps of the biosynthesis of primary and secondary metabolites for shoot growth and development. Seven enzymes (Aconitase, Succinate dehydrogenase, Pyruvate dehydrogenase, Fructose-6-phosphate 1-phosphotransgerase, Chacone synthase, Chalcone isomerase, and Monodehydroascorbate), one protein subunits (RuBisCo large subunit-binding protein) and one protein of Argonaute protein family (Tudor-Sn) supposedly involved with germinating potential of axillary buds and two enzymes (Hexokinase and Aldehyde dehydrogenase) supposedly involved with non-budding were highlighted.

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Co-Expression of Monodehydroascorbate Reductase and Dehydroascorbate Reductase from Brassica rapa Effectively Confers Tolerance to Freezing-Induced Oxidative Stress

Cited by 46 publications

References 62 publications

Proteomics provides insights into biological pathways altered by plant growth promoting bacteria and arbuscular mycorrhiza in sorghum grown in marginal soil

Proteomics provides insights into biological pathways altered by plant growth promoting bacteria and arbuscular mycorrhiza in sorghum grown in marginal soil

Expression of Heterologous OsDHAR Gene Improves Glutathione (GSH)-Dependent Antioxidant System and Maintenance of Cellular Redox Status in Synechococcus elongatus PCC 7942

Proteome of germinating and dormant axillary buds of sugarcane and analysis of sprouting-related proteins

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