Co-expression of cellulase and xylanase genes in Saccharomyces cerevisiae toward enhanced bioethanol production from corn stover

Abstract

Lignocellulose is considered as a good resource for producing renewable energy. Previous in vitro studies have shown the synergistic action between cellulase and xylanase during lignocellulose biohydrolysis. In order to achieve the same effect in S. cerevisiae to enhance the practical biotransformation, two recombinant Saccharomyces cerevisiae strains (INVSc1-CBH-CA and INVSc1-CBH-TS) with co-expressed cellulase and xylanase were constructed. The cellulase and xylanase activities in INVSc1-CBH-CA and INVSc1-CBH-TS were 716.43 U/mL and 205.13 U/mL, 931.27 U/mL and 413.70 U/ mL, respectively. The recombinant S. cerevisiae can use the partly delignified corn stover (PDCS) more efficiently and more ethanol producted than S. cerevisiae only expressing cellulase. Fermentation with INVSc1-CBH-CA and INVSc1-CBH-TS using PDCS ethanol yields increased by 1.7 and 2.1 folds higher than INVSc1-CBH, 2.8 and 3.4 folds higher than the wild type S. cerevisiae. The strategy of co-expression cellulase and xylanase in saccharomyces cerevisiae is effective and can be a foundation to research the mechanism of synergy effect of cellulose and xylanase.

Engineered Polyploid Yeast Strains Enable Efficient Xylose Utilization and Ethanol Production in Corn Hydrolysates

“…The pH of the system was adjusted to 4.8–5.0. Afterward, the distiller’s grains were hydrolyzed with 0.6% cellulase and 1.5% xylanase at 50°C and 250 rpm shaking for 24 h ( Hu et al, 2015 ; Xiao et al, 2019 ). Yeast cells were then used for fermentation at pH 4.6, 30°C and 150 rpm shaking (initial OD 600 of 1).…”

Apple orchard waste recycling and valorization of valuable product-A review

“…It resulted in substantial development of biogas with improved effluent capacity, reduced chemical oxygen demand by 96%. Xiao et al [ 25 ] digested tomato waste with cattle dung in an anaerobic reactor which also resulted in an increase from 0.33 to 0.700 dm 3 d −1 of biogas. Anaerobic treatment of fruit/vegetable waste is rich in organic matter and is beneficial as it produces more methane along with slurry which can be applied as a soil conditioner.…”

Consolidated Bioprocessing: Synthetic Biology Routes to Fuels and Fine Chemicals

“…It has the added advantage of containing the cellular machinery required for post-translational glycosylation of enzymes, enabling highly efficient fungal cellulases to be expressed and secreted in an active form. For example, one study described Saccharomyces cerevisiae strains which were engineered to secrete both a cellulase and a xylanase for efficient degradation of partly delignified corn stover [ 87 ]. The synergistic action of both enzymes increased ethanol titres by up to 3.4-fold compared to wild type S. cerevisiae .…”