Co-expression of C/EBPγ and ATF5 in mouse vomeronasal sensory neurons during early postnatal development

Nakano, Hitoo; Iida, Yoshitaka; Murase, Takahiro; Oyama, Natsuki; Umemura, Masayuki; Takahashi, Shigeru; Takahashi, Yûji

doi:10.1007/s00441-019-03070-2

Cited by 9 publications

(5 citation statements)

References 43 publications

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“…ATF5 knockout mice had abnormal olfactory system. Further investigation found that ATF5 was required for the differentiation, survival, and axonal projection of OSNs and V2r/Gαo‐type VSNs (Nakano et al, 2016, 2019; Umemura et al, 2015; Wang et al, 2012). Whether atf5 plays role in the development of OE in fish is unknown.…”

Section: Discussionmentioning

confidence: 99%

“…ATF5 was highly expressed in the mouse main olfactory epithelium (OE) and the mouse vomeronasal epithelium (VNE) from embryonic stage through adulthood (Nakano et al, 2016(Nakano et al, , 2019Wang et al, 2012). The majority of ATF5 deficient (ATF5 −/− ) pups died neonatally because these ATF5 knockout mice had abnormal olfactory system and failed to suckle milk (Wang et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Gene duplication, conservation, and divergence of activating transcription factor 5 gene in zebrafish

Zhu

Zhang

et al. 2022

J Exp Zool Pt B

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Activating transcription factor 5 (Atf5) is a member of the ATF/CREB family of transcription factors and involved in diverse cellular functions and diseases in mammals. However, the function of atf5 remains largely unknown in fish. Here, we report the expression pattern and function of duplicated atf5 genes in zebrafish. The results showed that the gene structures of zebrafish atf5a and atf5b were similar to their mammalian orthologs. Zebrafish Atf5a and Atf5b shared an amino acid sequence identity of 40.7%. Zebrafish atf5a and atf5b had maternal origin with dynamic expression during embryonic development. Zebrafish atf5a mRNA is mainly enriched in olfactory epithelium, midbrain, and hindbrain, while zebrafish atf5b mRNA is mainly detected in midbrain, hindbrain, and liver during embryogenesis. The results of acute hypoxia experiment showed that atf5a mRNA was significantly upregulated in the brain, liver, and muscle, while atf5b mRNA was just increased significantly in the brain. Functional analysis showed that knockdown of atf5a affects the development of the ciliated neurons in zebrafish embryos. The effect was enhanced when atf5a MO was co-injected with atf5b MO. The development of ciliated neurons in zebrafish embryos was not affected by injection of atf5b MO alone. atf5a knockdown also affects the development of early-born olfactory neurons. The effects caused by atf5a knockdown could be rescued by atf5b mRNA.These results suggest that the duplicated atf5 genes may have evolved divergently and play redundant biological roles in the development of olfactory sensory neurons in zebrafish.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Gene duplication, conservation, and divergence of activating transcription factor 5 gene in zebrafish

Zhu

Zhang

et al. 2022

J Exp Zool Pt B

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“…Neurons regenerate in adult animals 86,87 . Cell types in the vomeronasal lineage have been shown to be specified by BMP and Notch signaling 88,89 , and coordinated by transcription factors (TFs) including Bcl11b/Ctip2, C/EBPγ, ATF5, Gli3, Meis2, and Tfap2e [90][91][92][93][94][95] . However, the transcriptomic landscape that specifies the lineages is not known.…”

Section: Developmental Trajectories Of the Neuronal Lineagementioning

confidence: 99%

Molecular, Cellular, and Developmental Organization of the Mouse Vomeronasal Organ at Single Cell Resolution

Hills,

Ma,

Fang

et al. 2024

Preprint

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SUMMARYWe have generated single cell transcriptomic atlases of vomeronasal organs (VNO) from juvenile and adult mice. Combined with spatial molecular imaging, we uncover a distinct, previously unidentified class of cells that express the vomeronasal receptors and a population of canonical olfactory sensory neurons in the VNO. High resolution trajectory and cluster analyses reveal the lineage relationship, spatial distribution of cell types, and a putative cascade of molecular events that specify the V1r, V2r, and OR lineages from a common stem cell population. The expression of vomeronasal and olfactory receptors follow power law distributions, but there is high variability in average expression levels between individual receptor and cell types. Substantial co-expression is found between receptors across clades, from different classes, and between olfactory and vomeronasal receptors, with nearly half from pairs located on the same chromosome. Interestingly, the expression of V2r, but not V1r, genes is associated with various transcription factors, suggesting distinct mechanisms of receptor choice associated with the two cell types. We identify association between transcription factors, surface axon guidance molecules, and individual VRs, thereby uncovering a molecular code that guides the specification of the vomeronasal circuitry. Our study provides a wealth of data on the development and organization of the accessory olfactory system at both cellular and molecular levels to enable a deeper understanding of vomeronasal system function.

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“…Similarly, C/EBP β regulates the expression of genes involved in energy homeostasis and adipose tissue differentiation and affects endoplasmic reticulum (ER) stress and inflammation [ 94 , 95 ]. Although some studies have reported that C/EBP γ exerts transactivation effects, it mainly functions as an inhibitor of C/EBPs and other interacted transcription factors [ 20 , 25 , 96 ]. Meanwhile, C/EBP δ transcriptionally modulates various biological processes such as cell differentiation, proliferation, motility, growth arrest, cell death, and inflammation depending on the cell type and cellular context [ 31 , 32 , 88 ].…”

Section: Biological Features Of C/ebpsmentioning

confidence: 99%

CCAAT/Enhancer-Binding Proteins in Fibrosis: Complex Roles Beyond Conventional Understanding

et al. 2022

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CCAAT/enhancer-binding proteins (C/EBPs) are a family of at least six identified transcription factors that contain a highly conserved basic leucine zipper domain and interact selectively with duplex DNA to regulate target gene expression. C/EBPs play important roles in various physiological processes, and their abnormal function can lead to various diseases. Recently, accumulating evidence has demonstrated that aberrant C/EBP expression or activity is closely associated with the onset and progression of fibrosis in several organs and tissues. During fibrosis, various C/EBPs can exert distinct functions in the same organ, while the same C/EBP can exert distinct functions in different organs. Modulating C/EBP expression or activity could regulate various molecular processes to alleviate fibrosis in multiple organs; therefore, novel C/EBPs-based therapeutic methods for treating fibrosis have attracted considerable attention. In this review, we will explore the features of C/EBPs and their critical functions in fibrosis in order to highlight new avenues for the development of novel therapies targeting C/EBPs.

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Co-expression of C/EBPγ and ATF5 in mouse vomeronasal sensory neurons during early postnatal development

Cited by 9 publications

References 43 publications

Gene duplication, conservation, and divergence of activating transcription factor 5 gene in zebrafish

Gene duplication, conservation, and divergence of activating transcription factor 5 gene in zebrafish

Molecular, Cellular, and Developmental Organization of the Mouse Vomeronasal Organ at Single Cell Resolution

CCAAT/Enhancer-Binding Proteins in Fibrosis: Complex Roles Beyond Conventional Understanding

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