Co-expression of activating and inhibitory receptors on peritoneal fluid NK cells in women with endometriosis

Saeki, Shinichiro; Fukui, Atsushi; Mai, Chuxian; Takayama, Ryu; Yamaya, Ayano; Shibahara, Hiroaki

doi:10.1016/j.jri.2022.103765

Cited by 4 publications

(12 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The remaining 22 articles were thoroughly analyzed and read in full, leading to the exclusion of 4 more studies due to research theme incongruency. In total, 18 studies [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20]24,25] were included in the final qualitative analysis, as per Figure 2.…”

Section: Study Selectionmentioning

confidence: 99%

“…Quality assessment was conducted on the 18 selected studies as presented in Table S3 (following NHLBI Assessment Tool guidelines). Among these studies, 15 were deemed "good quality" [5,6,[8][9][10][14][15][16][17][18][19][20]24,25]. This assessment was based on the sample size, consideration/adjustment to key confounding variables (demographic factors, menstrual cycle stage, hormonal therapy, and disease staging as per revised American Society for Reproductive Medicine (rASRM) classification) [23], and appropriateness of the methodology according to the research hypothesis.…”

Section: Study Quality Assessment and Risk Of Biasmentioning

confidence: 99%

“…Heterogenicity was noted in the samples evaluated in each study, as presented in Table 1. Studies used a wide array of samples, including peritoneal fluid [6][7][8][9][10][11]13,14,[16][17][18][19]24,25], endometriotic lesions/eutopic endometrium samples [12,15,18,19,24], and peripheral blood [7][8][9][10]18,20,24].…”

Section: Heterogenicity Of the Studies Assessedmentioning

confidence: 99%

“…Additionally, each study employed different techniques to evaluate the NK cell receptor activity and expression in endometriosis. Flow cytometry [7][8][9][10][11]14,16,19,24,25] was most commonly used, followed by Western blotting [6][7][8][9][10]19,24], immunohistochemistry [12,15,18,24], polymerase chain reaction (PCR) [14,19,20], enzyme-linked immunosorbent assay (ELISA) [13,17,18], RNA in situ hybridization [12], and 51 Cr cytotoxicity assays [5].…”

Section: Heterogenicity Of the Studies Assessedmentioning

confidence: 99%

“…Fourteen studies evaluated the activity and expression of NK cell receptors in the peritoneal fluid of endometriosis subjects [5][6][7][8][9][10][11]13,14,[16][17][18][19]25]. The role of immune dysfunction in the progression of endometriosis was highlighted by Maeda and colleagues [7][8][9], who found, by using flow cytometry, that the levels of NK cell inhibitory receptors such as CD158a+ and KIR2DL1+ were significantly higher in the peritoneal fluid of endometriosis subjects than controls.…”

Section: Nk Cell Receptors In Peritoneal Fluidmentioning

confidence: 99%

See 4 more Smart Citations

Natural Killer Cell Receptors and Endometriosis: A Systematic Review

Reis

Rosa

Ângelo-Dias

et al. 2022

IJMS

View full text Add to dashboard Cite

Endometriosis is a chronic inflammatory disorder, characterized by the presence of endometrial cells outside the uterine cavity. An increasing number of studies correlate the immune system with endometriosis, particularly NK receptors (NKR), which have been suggested to play an essential role in the pathogenesis of the disease. This systematic review aims to enlighten the role of NKR in endometriosis. A literature search was performed independently by two reviewers, to identify studies assessing the role of NKR in endometriosis. In total, 17 studies were included. Endometriosis pathogenesis seems to be marked by the overexpression of NK inhibitor receptors (KIRS), namely, CD158a+, KIR2DL1, CD94/NKG2A, PD-1, NKB1, and EB6, and inhibiting ligands such as PD-L1, HLA-E, HLA-G, and HLA-I. Concurrently, there is a decrease in NK-activating receptors and natural cytotoxicity receptors (NCRs), such as NKp46, NKp30, and NKG2D. The immune shift from NK surveillance to NK suppression is also apparent in the greater relative number of ITIM domains compared with ITAM domains in NKRs. In conclusion, NK receptor activity seems to dictate the immunocompetency of women to clear endometriotic cells from the peritoneal cavity. Future research could explore NKRs as therapeutic targets, such as that which is now well established in cancer therapy through immunotherapy.

show abstract

Section: Study Selectionmentioning

confidence: 99%

Section: Study Quality Assessment and Risk Of Biasmentioning

confidence: 99%

Section: Heterogenicity Of the Studies Assessedmentioning

confidence: 99%

Section: Heterogenicity Of the Studies Assessedmentioning

confidence: 99%

Section: Nk Cell Receptors In Peritoneal Fluidmentioning

confidence: 99%

See 3 more Smart Citations

Natural Killer Cell Receptors and Endometriosis: A Systematic Review

Reis

Rosa

Ângelo-Dias

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

Synergy between Th1 and Th2 responses during endometriosis: A review of current understanding

L.A.

Shan

Hou

et al. 2023

Journal of Reproductive Immunology

View full text Add to dashboard Cite

Combined with bioinformatics and machine learning, the diagnostic model, Immunological features and subtypes of stage IV endometriosis with infertility were analyzed

Lin,

Long,

et al. 2024

Preprint

View full text Add to dashboard Cite

Many studies have shown that endometriosis can lead to infertility in women of reproductive age, but the mechanism is unknown. Our study aims to explore the pathogenesis of stage IV endometriosis with infertility and the role of characteristic genes in this condition. Methods Gene expression profiles were obtained from the GSE120103 dataset retrieved from the GEO database. Weighted gene co-expression network analysis (WGCNA) was used to identify key modules. Subsequently, minimum absolute contraction, selection operator (LASSO), and random forest machine learning algorithms were employed to screen the characteristic genes of stage IV endometriosis complicated with infertility. The ROC curve and diagnostic model were generated to evaluate the diagnostic efficacy. CIBERSORT was utilized to estimate immune cell infiltration and quantify immune checkpoints. Additionally, we constructed the regulatory network of miRNA and transcription factors.GSEA was utilized to explore the signaling pathways associated with characteristic genes, and potential small molecule compounds were identified through screening the CTD database. Samples from individuals with infertility in stage IV endometriosis were categorized using the consensus clustering method, followed by an examination of the expression and immunological features of different subtypes. Results We identified five characteristic genes (CDY2A, KRT6B, SLC2A2, SRY, MYH7) that predict infertility in stage IV endometriosis. When compared to women of childbearing age with stage IV endometriosis, the immunological features of stage IV endometriosis combined with infertility show significant differences, which are clearly linked to the characteristic genes. Patients can benefit from a gene-based characteristic nomogram. Our study reveals that multiple signaling pathways are strongly associated with infertility in stage IV endometriosis. Furthermore, several small molecule compounds were predicted based on the characteristic genes, and relevant regulatory networks of miRNA and TF were constructed. Stage IV endometriosis combined with infertility is categorized into three subtypes, each showing significantly different immunological characteristics of the characteristic genes. Conclusion This study enhances our understanding of the pathogenesis and immune mechanisms of stage IV endometriosis with infertility. It identifies effective characteristic genes and subtypes, offering valuable insights for treatment. Nevertheless, additional prospective studies and experiments are necessary to validate our findings.

show abstract

Co-expression of activating and inhibitory receptors on peritoneal fluid NK cells in women with endometriosis

Cited by 4 publications

References 33 publications

Natural Killer Cell Receptors and Endometriosis: A Systematic Review

Natural Killer Cell Receptors and Endometriosis: A Systematic Review

Synergy between Th1 and Th2 responses during endometriosis: A review of current understanding

Combined with bioinformatics and machine learning, the diagnostic model, Immunological features and subtypes of stage IV endometriosis with infertility were analyzed

Contact Info

Product

Resources

About