Co-exposure to titanium dioxide nanoparticles does not affect cadmium toxicity in radish seeds (Raphanus sativus)

Manesh, R. Roshan; Grassi, Giacomo; Bergami, Elisa; Marques‐Santos, Luis Fernando; Faleri, Claudia; Liberatori, Giulia; Corsi, Ilaria

doi:10.1016/j.ecoenv.2017.10.051

Cited by 50 publications

(19 citation statements)

References 41 publications

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“…Similarly, Manesh et al . 44 reported that germination index and root length were affected by TiO 2 nanoparticle (n-TiO 2 ) exposure. A significant reduction in root elongation and germination percentage was observed in seeds with co-exposure to n-TiO2 and CdCl2 at the highest concentrations (1000 mg/L and 250 mg/L, respectively) as compared to co-exposure at lower concentrations (1 mg/L and 1 mg/L, respectively) and controls (p < 0.05).…”

Section: Resultsmentioning

confidence: 99%

CTAB Surfactant Assisted and High pH Nano-Formulations of CuO Nanoparticles Pose Greater Cytotoxic and Genotoxic Effects

Singh

2019

Sci Rep

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Toxicity of synthesized nanoparticles is the area of concern to all the researchers due to their possible health implications. Here we synthesized copper oxide nanoparticles (CuO NPs) without surfactant at pH value of 2, 7, 10 and with cetyletrimethylammoniumbromide (CTAB) surfactant at pH 7. Synthesized nanoparticles were characterized for various structural parameters including crystallite size, lattice parameters, strain, phase analysis using X-ray diffraction analysis, and morphological aspects have been analyzed using FESEM and HRTEM imaging. All the four nano-formulations were analyzed for their toxic potential using Allium cepa L. at three different concentrations (0.1, 0.01 and 0.001 g/100 ml). Cytological and genetic parameters including mitotic index, mitotic inhibition, aberrant cells, binucleated cells, micronucleated cells, chromosomal bridges, fragmentation, stickiness, laggards, vagrants, c-mitosis and disturbed spindle were analyzed. Our results revealed a dose dependent increase in cytotoxic parameters including decreased total dividing cells, mitotic index, and increased mitotic inhibition. Genotoxic parameters also increased at higher treatment concentrations including chromosomal aberrations and percent aberrant cells. The pH value at the time of particle synthesis has significant influence on the crystallite size and agglomeration as assessed by XRD, FESEM and HRTEM analysis. The NPs synthesized at pH 2 and 10 were found to be of smaller size and posed more toxic effects as compared to particles synthesized at neutral pH. On the other hand, CTAB assisted CuO NPs synthesized at pH 7 revealed even smaller crystallite sizes and thus boost the toxicity in all the parameters as compared to NPs synthesized without CTAB. The present study suggested an increase in toxic parameters of synthesized CuO NPs with respect to crystallite size which is pH dependent. Addition of CTAB at pH 7 decreased the crystallite as well as particle size and enhanced the toxic potential. Further studies are recommended to analyze the effect of surfactant addition in toxicological studies on CuO NPs.

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Section: Resultsmentioning

confidence: 99%

CTAB Surfactant Assisted and High pH Nano-Formulations of CuO Nanoparticles Pose Greater Cytotoxic and Genotoxic Effects

Singh

2019

Sci Rep

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“…Currently, germination rate and shoot-root elongation are rapid tests that are actively used to determine the phytotoxicity of NPs on various plant species [29][30][31]. This test has several advantages such as sensitivity, simplicity, low cost, and suitability also for unstable NPs [32].…”

Section: Discussionmentioning

confidence: 99%

Effects of Fe₃O₄ Nanoparticle Stress on the Growth and Development of Rocket Eruca sativa

Plaksenkova

Jermaļonoka

Bankovska

et al. 2019

Journal of Nanomaterials

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Plants exposed to stress use the variety of gene regulatory mechanisms to achieve cellular homeostasis, including posttranscriptional regulation of gene expression where microRNAs (miRNAs) play a pivotal role. Since various environmental stress factors such as nanoparticles affect crop productivity and quality, the aim of the present study was to evaluate the genotoxicity level and to estimate miRNA expression level and chlorophyll a level in the magnetite (Fe3O4) nanoparticle-stressed rocket (Eruca sativa Mill.) seedlings grown in hydroponics. Rocket seedlings were exposed to 1 mg/L, 2 mg/L, and 4 mg/L Fe3O4 nanoparticles, and after 5 weeks, seed germination rate, root-shoot elongation, genotoxicity, chlorophyll a, and miRNA expression levels were evaluated. The obtained results indicated that 1 mg/L, 2 mg/L, and 4 mg/L concentrations of Fe3O4 nanoparticles induce low genotoxicity and have a positive effect on the growth and development of rocket seedlings and that nanoparticles may improve the ability of plants to stand against environmental stresses.

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“…Cd-induced damage depends on the dose, duration of exposure, type of contact, as well as the interaction with other materials and/or nanomaterials. The effects of TiO 2 NPs and Cd 2+ co-exposure have been investigated in plants and aquatic species [ 6 , 40 , 41 , 42 , 43 , 44 , 45 , 46 ]. A recent study showed that a non-cytotoxic concentration of TiO 2 NPs enhanced the toxicological potential of Cd 2+ in human liver (HepG2) and human breast cancer (MCF-7) cells [ 47 ].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Effects of Titanium Dioxide Nanoparticles (TiO2NPs) on Cadmium Chloride (CdCl2) Genotoxicity in Human Sperm Cells

et al. 2020

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The environmental release of titanium dioxide nanoparticles (TiO2NPs) associated with their intensive use has been reported to have a genotoxic effect on male fertility. TiO2NP is able to bind and transport environmental pollutants, such as cadmium (Cd), modifying their availability and/or toxicity. The aim of this work is to assess the in vitro effect of TiO2NPs and cadmium interaction in human sperm cells. Semen parameters, apoptotic cells, sperm DNA fragmentation, genomic stability and oxidative stress were investigated after sperm incubation in cadmium alone and in combination with TiO2NPs at different times (15, 30, 45 and 90 min). Our results showed that cadmium reduced sperm DNA integrity, and increased sperm DNA fragmentation and oxidative stress. The genotoxicity induced by TiO2NPs-cadmium co-exposure was lower compared to single cadmium exposure, suggesting an interaction of the substances to modulate their reactivity. The Quantitative Structure-Activity Relationship (QSAR) computational method showed that the interaction between TiO2NPs and cadmium leads to the formation of a sandwich-like structure, with cadmium in the middle, which results in the inhibition of its genotoxicity by TiO2NPs in human sperm cells.

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Co-exposure to titanium dioxide nanoparticles does not affect cadmium toxicity in radish seeds (Raphanus sativus)

Cited by 50 publications

References 41 publications

CTAB Surfactant Assisted and High pH Nano-Formulations of CuO Nanoparticles Pose Greater Cytotoxic and Genotoxic Effects

CTAB Surfactant Assisted and High pH Nano-Formulations of CuO Nanoparticles Pose Greater Cytotoxic and Genotoxic Effects

Effects of Fe₃O₄ Nanoparticle Stress on the Growth and Development of Rocket Eruca sativa

In Vitro Effects of Titanium Dioxide Nanoparticles (TiO2NPs) on Cadmium Chloride (CdCl2) Genotoxicity in Human Sperm Cells

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Co-exposure to titanium dioxide nanoparticles does not affect cadmium toxicity in radish seeds (Raphanus sativus)

Cited by 50 publications

References 41 publications

CTAB Surfactant Assisted and High pH Nano-Formulations of CuO Nanoparticles Pose Greater Cytotoxic and Genotoxic Effects

CTAB Surfactant Assisted and High pH Nano-Formulations of CuO Nanoparticles Pose Greater Cytotoxic and Genotoxic Effects

Effects of Fe3O4 Nanoparticle Stress on the Growth and Development of Rocket Eruca sativa

In Vitro Effects of Titanium Dioxide Nanoparticles (TiO2NPs) on Cadmium Chloride (CdCl2) Genotoxicity in Human Sperm Cells

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Effects of Fe₃O₄ Nanoparticle Stress on the Growth and Development of Rocket Eruca sativa