Co-existence of NDM-1 and OXA-48 genes in Carbapenem Resistant Klebsiella pneumoniae clinical isolates in Kafrelsheikh, Egypt

El-Domany, Ramadan A.; El-Banna, Tarek; Sonbol, Fatma Ibrahim; Abu-Sayedahmed, Samar Hamed

doi:10.4314/ahs.v21i2.2

Cited by 26 publications

(29 citation statements)

References 49 publications

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“…The current study revealed that 76% of isolates were pan-drug resistant, 17% were extreme-drug resistant, and 7% were multidrug resistant. In addition, another study in Egypt detected that all 50 CRKP isolates were multidrug resistant (MDR) [ 24 ]. These values may be attributable to the absence of an appropriate antibiotic strategy used for VAP patients, ensuing long and improper use of antimicrobial agents, which has ultimately resulted in marked antibiotic resistance.…”

Section: Discussionmentioning

confidence: 99%

“…Tawfick et al detected that bla NDM was the most prevalent genotype of carbapenemases by 68.88% (93/135). Furthermore, El-Domany et al stated that the incidence of bla NDM was 70.0% [ 24 , 28 ]. On the other hand, Ghaith et al, detected that the most prevalent carbapenemase gene was bla OXA-48 which was identified in 14/23 (60.8%) isolates followed by bla NDM , which was identified in 12/23 (52.2%) isolates [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

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Characteristics of Carbapenem-Resistant Gram-Negative Bacilli in Patients with Ventilator-Associated Pneumonia

et al. 2021

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Carbapenem-resistant Gram-negative bacilli (CR-GNB) has become a global threat. In hospital settings, the association of CR-GNB with ventilator-associated pneumonia (VAP) is a critical public health concern owing to their high resistance rate to most antibiotics. The present study aims to identify the frequency of carbapenem-resistance and to determine the rate of multidrug resistance (MDR), extensive drug resistance (XDR) and pan-drug resistance (PDR) among CR-GNB infections in VAP. Antimicrobial susceptibility testing was carried out using the disk diffusion method and the detection of carbapenemases was screened using the imipenem-E test and the modified carbapenem-inactivation method (mCIM). The isolates were verified by polymerase chain reaction (PCR) for the presence of blaNDM, blaSPM, blaVIM, blaIMP and blaGIM genes. 89.5%, 14%, 17.5%, 10.5%, 3.5% of isolates exhibited the presence of blaNDM, blaVIM, blaSPM, blaIMP and blaGIM, respectively. 76%, 17% and 7% of isolates were PDR, XDR, and MDR, respectively. Carbapenem-resistance genes were identified in a significant percentage and blaNDM was the most predominant gene. All isolates were highly resistant to most antibiotics. This health concern has proven to be a big challenge in developing countries such as Egypt, as it is associated with high morbidity, high mortality, and raised healthcare costs.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Characteristics of Carbapenem-Resistant Gram-Negative Bacilli in Patients with Ventilator-Associated Pneumonia

et al. 2021

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“…considered as rarely reported in Tunisia(Messaoudi et al 2017;Dziri et al 2019). In contrast, a coproduction of multiple types of carbapenemases by the same strain is more frequently reported in previous studies from several countries(Papagiannitsis et al 2017;Hamel et al 2020;El-Domany et al 2021). Recent studies from Egypte, Morocco and Thailand have reported that blaNDM-1+blaOXA-48 genes were one of the most prevalent carbapenemases genes co-existence among K. pneumoniae isolates with high incidence ranging from 24% to 48Á0%(Laolerd et al 2018;El-Domany et al 2021;Loqman et al 2021).…”

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confidence: 94%

Co-occurrence of genes encoding carbapenemase, ESBL, pAmpC and non-β-Lactam resistance among Klebsiella pneumonia and E. coli clinical isolates in Tunisia

Sallem

Laribi

Arfaoui

et al. 2022

Letters in Applied Microbiology

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This study aimed to investigate the molecular mechanisms of carbapenem and colistin resistance in K. pneumoniae and E. coli isolates obtained from hospitalized patients in Carthagene International Hospital of Tunis. A total of 25 K. pneumoniae and 2 E. coli clinical isolates with reduced susceptibility to carbapenems were recovered. Susceptibility testing and phenotypic screening tests were carried out. ESBL, AmpC, carbapenemase and other antibiotic resistance genes were sought by PCR‐sequencing. The presence of plasmid‐mediated colistin resistance genes (mcr‐1‐8) was examined by PCR and the nucleotide sequence of the mgrB gene was determined. The analysis of plasmid content was performed by PCR‐Based Replicon Typing (PBRT). The clonality of isolates was assessed by PFGE and multilocus sequence typing (MLST). All of the isolates produced carbapenemase activity. They showed a great variation in the distribution of ESBL, AmpC, carbapenemase and other plasmid‐mediated resistance determinants. K. pneumoniae isolates carried blaNDM‐1 (n = 11), blaOXA‐48 (n = 11), blaNDM‐1 + blaOXA‐48 (n = 1), blaNDM‐1 + blaVIM‐1 (n = 1), blaOXA‐204 (n = 1), along with blaCTX‐M, blaOXA, blaTEM, blaCMY, blaDHA and blaSHV genes variants on conjugative plasmid of IncL/M, IncR, IncFIIK, IncFIB, and IncHI1 types. Three sequence types ST101, ST307 and ST15 were identified. The mgrB alteration g109a (G37S) was detected in a single colistin‐resistant, NDM‐1 and OXA‐48‐coproducing K. pneumoniae isolate. The two E. coli isolates belonged to ST95, co‐produced NDM‐1 and CTX‐M‐15, and harboured plasmid of IncFII and IncFIB types. To our knowledge, this is the first report in Tunisia of NDM‐1, OXA‐48, and CTX‐M‐15 coexistence in colistin‐resistant K. pneumoniae ST15.

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“…PCR results for the commonly reported carbapenemase genes in Enterobacterales revealed that 80.8% of the isolates harbored carbapenemase genes, which is in concordance with another Egyptian study where carbapenemase genes were detected in 74% of the isolates. 30 The most prevalent gene was bla NDM (50%) followed by the bla OXA-48 (36.5%). This finding agrees with another study on Enterobacterales from Egyptian cancer patients, 31 but Raheel et al reported bla OXA-48 as the most common gene followed by bla NDM .…”

Section: Discussionmentioning

confidence: 98%

Carbapenem-Resistant Klebsiella pneumoniae Among Patients with Ventilator-Associated Pneumonia: Evaluation of Antibiotic Combinations and Susceptibility to New Antibiotics

Ramadan¹,

Bedawy²,

Negm³

et al. 2022

IDR

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Co-existence of NDM-1 and OXA-48 genes in Carbapenem Resistant Klebsiella pneumoniae clinical isolates in Kafrelsheikh, Egypt

Cited by 26 publications

References 49 publications

Characteristics of Carbapenem-Resistant Gram-Negative Bacilli in Patients with Ventilator-Associated Pneumonia

Characteristics of Carbapenem-Resistant Gram-Negative Bacilli in Patients with Ventilator-Associated Pneumonia

Co-occurrence of genes encoding carbapenemase, ESBL, pAmpC and non-β-Lactam resistance among Klebsiella pneumonia and E. coli clinical isolates in Tunisia

Carbapenem-Resistant Klebsiella pneumoniae Among Patients with Ventilator-Associated Pneumonia: Evaluation of Antibiotic Combinations and Susceptibility to New Antibiotics

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