Co-distribution of von Willebrand factor and fibronectin in cultured rhesus endothelial cells

Abstract

The synthesis and secretion of von Willebrand factor (VWF, or Factor VIII-related antigen) and fibronectin by cultured endothelial cells from rhesus monkey choroid retina were demonstrated by immunofluorescence, immunoperoxidase and single radial immunodiffusion techniques. Both VWF and fibronectin are localized in intracellular granules and extracellular fibrils. The results of double immunofluorescence staining and post-embedding immunoelectron microscopy showed that there was a co-distribution of VWF and fibronectin not only in pericellular fibrils where they co-aligned with each other to be the components of extracellular matrix, but also in intracellular granules, suggesting they were synthesized or translocated in the same compartment.

Insulin-Like Growth Factor Binding Protein-Related Protein 1 Mediates VEGF-Induced Proliferation, Migration and Tube Formation of Retinal Endothelial Cells

“…It has been identified as being of endothelial origin by cellular morphology, growth pattern, ultrastructure, immunocytochemistry, and immunodiffusion. 20,21 We confirmed that RF/6A cells did express IGFBP-rP1 transcript-specific amplicon, and this level of transcription correlated well with the expression of IGFBP-rP1 protein, as demonstrated by Western blot analysis. In addition, IGFBP-rP1 appeared to be constitutively expressed in human retinal pigment epithelial cells.…”

Senescence Increases Choroidal Endothelial Stiffness and Susceptibility to Complement Injury: Implications for Choriocapillaris Loss in AMD

“…Conceptually, our observation that a cell line (RF/6A) deemed to be immortal exhibits markers of senescence at high passages appears paradoxical. However, in support of our observations, we would like to point out that RF/6A ECs have conventionally been used between passages 18 through 60 [42][43][44] while the studies that used these higher passage cells never assessed the markers of senescence. Thus, to our knowledge, we are the first to use RF/6A ECs past the conventional passage number and measure expression of senescence markers.…”

Cysteine-rich 61 (CYR61) is up-regulated in proliferative diabetic retinopathy

“…These cells had been identified as being of endothelial origin by cellular morphology, growth pattern, ultrastructure, immunocytochemistry, and immunodiffusion. The use of the RF/6A cell line as retinal or choroidal endothelial cells for studying the pathogenesis and prevention of neovascular diseases has proved to be a reliable in vitro model [15][16][17]. We have proved that CYR61 can promote proliferation, migration, and tube formation of RF/6A cells, and these physiological functions are the basis of neovascularization.…”