Co-delivery of doxorubicin and recombinant plasmid pHSP70-Plk1-shRNA by bacterial magnetosomes for osteosarcoma therapy

Cheng, Li; Ke, Youqun; Yu, Shuisheng; Jing, Juehua

doi:10.2147/ijn.s115364

Cited by 43 publications

(26 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, the link between magnetosomes and the anticancer recombinant plasmid heat shock protein, 70‐polo‐like kinase 1‐short hairpin RNA, as well as doxorubicin are based on charge interactions. [ 331 ] Because short‐interfering ribonucleic acid (siRNA) is capable of silencing homologous gene expressions, they have been conjugated to magnetosomes via polyethyleneimine for the intracellular delivery of siRNA to cancer cells to inhibit their growth in a dose‐ and time‐dependent manner (Figure 16D). [ 332 ]…”

Section: Applicationsmentioning

confidence: 99%

Microbe‐Mediated Extracellular and Intracellular Mineralization: Environmental, Industrial, and Biotechnological Applications

et al. 2020

View full text Add to dashboard Cite

Bacteria play an important role in the calcification process of natural minerals and within organisms ( Table 1). It is Microbe-mediated mineralization is ubiquitous in nature, involving bacteria, fungi, viruses, and algae. These mineralization processes comprise calcification, silicification, and iron mineralization. The mechanisms for mineral formation include extracellular and intracellular biomineralization. The mineral precipitating capability of microbes is often harnessed for green synthesis of metal nanoparticles, which are relatively less toxic compared with those synthesized through physical or chemical methods. Microbe-mediated mineralization has important applications ranging from pollutant removal and nonreactive carriers, to other industrial and biomedical applications. Herein, the different types of microbe-mediated biomineralization that occur in nature, their mechanisms, as well as their applications are elucidated to create a backdrop for future research.

show abstract

Section: Applicationsmentioning

confidence: 99%

Microbe‐Mediated Extracellular and Intracellular Mineralization: Environmental, Industrial, and Biotechnological Applications

et al. 2020

View full text Add to dashboard Cite

show abstract

“…It is important to note here that RFPECs are ideal for this study, because unlike many other EC types RFPECs express a robust GCX [60, 61]. Furthermore, similar to other EC types, RFPECs properly respond to the application of shear stress [62, 63] and have been shown to elicit atherosclerosis relevant GCX function [64–67], F-actin expression, morphology, and alignment [62, 68], intercellular communication [62, 69], endothelium permeability [70, 71], and NO production [72]. Acknowledging that ECs are heterogenous due to their origins from different species and different vascular beds [73], we wish to indicate that we are conducting parallel studies with primary endothelium originating from other species, including human, and from a variety of vascular beds, including the aorta and coronary arteries.…”

Section: Methodsmentioning

confidence: 99%

Pro-atherosclerotic disturbed flow disrupts caveolin-1 expression, localization, and function via glycocalyx degradation

et al. 2018

View full text Add to dashboard Cite

BackgroundEndothelial-dependent atherosclerosis develops in a non-random pattern in regions of vessel bending and bifurcations, where blood flow exhibits disturbed flow (DF) patterns. In contrast, uniform flow (UF), normal endothelium, and healthy vessel walls co-exist within straight vessels. In clarifying how flow protectively or atherogenically regulates endothelial cell behavior, involvement of the endothelial surface glycocalyx has been suggested due to reduced expression in regions of atherosclerosis development. Here, we hypothesized that pro-atherosclerotic endothelial dysfunction occurs as a result of DF-induced reduction in glycocalyx expression and subsequently impairs endothelial sensitivity to flow. Specifically, we propose that glycocalyx degradation can induce pro-atherosclerotic endothelial dysfunction through decreased caveolin-1 and endothelial nitric oxide synthase expression and localization.MethodsWe studied endothelial cells in atherosclerotic-prone DF and atherosclerotic-resistant UF conditions in parallel plate flow culture and in C57Bl/6 mice. The effects of flow conditioning on endothelial cell behavior were quantified using immunocytochemistry. The glycocalyx was fluorescently labeled for wheat germ agglutinin, which serves as a general glycocalyx label, and heparan sulfate, a major glycocalyx component. Additionally, mechanosensitivity was assessed by immunocytochemical fluorescence expression and function of caveolin-1, the protein that forms the mechanosignaling caveolar invaginations on the endothelial surface, total endothelial-type nitric oxide synthase (eNOS), which synthesizes nitric oxide, and serine 1177 phosphorylated eNOS (eNOS-pS1177), which is the active form of eNOS. Caveolin function and eNOS expression and activation were correlated to glycocalyx expression. Heparinase III enzyme was used to degrade a major glycocalyx component, HS, to identify the role of the glycocalyx in caveoin-1 and eNOS-pS1177 regulation.ResultsResults confirmed that DF reduces caveolin-1 expression and abolishes most of its subcellular localization preferences, when compared to the effect of UF. DF down-regulates caveolin-1 mechanosignaling, as indicated by its reduced colocalization with serine 1177 phosphorylated endothelial-type nitric oxide synthase (eNOS-pS1177), a vasoregulatory signaling molecule whose activity is regulated by its residence in caveolae. As expected, DF inhibited glycocalyx expression compared to UF. In the absence of heparan sulfate, a major glycocalyx component, UF-conditioned endothelial cells exhibited near DF-like caveolin-1 expression, localization, and colocalization with eNOS-pS1177.ConclusionsThis is the first demonstration of a flow-defined role of the glycocalyx in caveolae expression and function related to vasculoprotective endothelial mechanosensitivity that defends against atherosclerosis. The results suggest that a glycocalyx-based therapeutic targeted to areas of atherosclerosis development could prevent disease initiation and progression.Electronic suppleme...

show abstract

“…Despite the fact that most methods of magnetosome functionalization utilize amine crosslinking, the overall negative surface charge of magnetosomes, caused by the phospholipids of the magnetosome membrane, enables a strong interaction with positively charged, coating macromolecules. For example, Cheng et al (2016) used polyethylenimine to establish a link between the anticancer recombinant plasmid phsP70-Plk1-shrNa and the magnetosome surface together with doxorubicin [ 74 ]. Poly-aminoacids, such as poly-glutamic acid, are also potential tools for conjugation functional molecules onto magnetosomes, again based on charge interactions [ 65 ].…”

Section: Resultsmentioning

confidence: 99%

“…The use of hyperthermia in the treatment of cancers is appealing because it does not have the toxic side effects and thus is less restrictive than chemotherapy and radiotherapy, and it could even be used in combination with these therapies thereby increasing treatment efficiency [ 120 ]. In the hyperthermia procedure, tumor cells are destroyed and the tumor reduced in size or eliminated completely by increasing the temperature within the tumor typically within the range of 37–45 °C [ 74 , 100 , 101 , 121 , 122 , 123 , 124 , 125 ]. Superparamagnetic iron oxide nanoparticles (SION) are artificially synthesized nanoparticles used for hyperthermia and are often limited in effectiveness by the inability to place them specifically and solely within the targeted tumor tissue.…”

Section: Resultsmentioning

confidence: 99%

Applications of Magnetotactic Bacteria, Magnetosomes and Magnetosome Crystals in Biotechnology and Nanotechnology: Mini-Review

et al. 2018

View full text Add to dashboard Cite

Magnetotactic bacteria (MTB) biomineralize magnetosomes, which are defined as intracellular nanocrystals of the magnetic minerals magnetite (Fe3O4) or greigite (Fe3S4) enveloped by a phospholipid bilayer membrane. The synthesis of magnetosomes is controlled by a specific set of genes that encode proteins, some of which are exclusively found in the magnetosome membrane in the cell. Over the past several decades, interest in nanoscale technology (nanotechnology) and biotechnology has increased significantly due to the development and establishment of new commercial, medical and scientific processes and applications that utilize nanomaterials, some of which are biologically derived. One excellent example of a biological nanomaterial that is showing great promise for use in a large number of commercial and medical applications are bacterial magnetite magnetosomes. Unlike chemically-synthesized magnetite nanoparticles, magnetosome magnetite crystals are stable single-magnetic domains and are thus permanently magnetic at ambient temperature, are of high chemical purity, and display a narrow size range and consistent crystal morphology. These physical/chemical features are important in their use in biotechnological and other applications. Applications utilizing magnetite-producing MTB, magnetite magnetosomes and/or magnetosome magnetite crystals include and/or involve bioremediation, cell separation, DNA/antigen recovery or detection, drug delivery, enzyme immobilization, magnetic hyperthermia and contrast enhancement of magnetic resonance imaging. Metric analysis using Scopus and Web of Science databases from 2003 to 2018 showed that applied research involving magnetite from MTB in some form has been focused mainly in biomedical applications, particularly in magnetic hyperthermia and drug delivery.

show abstract

Co-delivery of doxorubicin and recombinant plasmid pHSP70-Plk1-shRNA by bacterial magnetosomes for osteosarcoma therapy

Cited by 43 publications

References 36 publications

Microbe‐Mediated Extracellular and Intracellular Mineralization: Environmental, Industrial, and Biotechnological Applications

Microbe‐Mediated Extracellular and Intracellular Mineralization: Environmental, Industrial, and Biotechnological Applications

Pro-atherosclerotic disturbed flow disrupts caveolin-1 expression, localization, and function via glycocalyx degradation

Applications of Magnetotactic Bacteria, Magnetosomes and Magnetosome Crystals in Biotechnology and Nanotechnology: Mini-Review

Contact Info

Product

Resources

About