Co-delivery of curcumin-loaded nanoemulsion and Phaleria macrocarpa extract to NIH 3T3 cell for antifibrosis

Rachmawati, Heni; Novel, Miranti A.; Nisa, Risya M.; Berlian, Guntur; Tandrasasmita, Olivia Mayasari; Rahma, Annisa; Riani, Catur; Tjandrawinata, Raymond R.

doi:10.1016/j.jddst.2017.03.015

Cited by 14 publications

(10 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Both natural compounds were suggested to have synergism effect in the in vitro model of liver fibrosis. The co-encapsulation of curcumin and silymarin in nanoemulsion enhanced their activities, which was indicated by the reduction of collagen type IV expression in mouse and NIH/3T3 cell line at gene as well as protein levels [14]. In agreement with our results, other reports also described the potential use of curcumin as an antifibrotic agent, which have been shown in different animal models of liver fibrosis [15][16][17][18].…”

Section: Introductionsupporting

confidence: 91%

“…The curcumin antifibrotic activity on inhibiting collagen synthesis by NIH/3T3 cells has been shown previously by our group [14]. The antifibrotic activity of curcumin might be due to its antioxidant and anti-inflammatory activity.…”

Section: Discussionsupporting

confidence: 66%

“…In the previous work, our group has successfully developed nanocarrier based curcumin and silymarin [14]. Both natural compounds were suggested to have synergism effect in the in vitro model of liver fibrosis.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Antifibrotic Activity and In Ovo Toxicity Study of Liver-Targeted Curcumin-Gold Nanoparticle

et al. 2018

Self Cite

View full text Add to dashboard Cite

Conjugation of curcumin and gold with green chemistry is an approach to improve the effectiveness of curcumin as anti-fibrosis. In this work, curcumin and gold were conjugated to deliver curcumin to the liver. Curcumin-gold nanoparticles (cAuNPs) were prepared by varying curcumin pH and concentration. The successful of cAuNPs formation were identified by using UV-visible and FTIR spectrophotometers. The particle size and morphology were analyzed using particle size analyzer and cryo-TEM respectively. In vitro antioxidant assay was performed to determine the curcumin activity after conjugation. Physical and chemical stabilities of cAuNPs were studied for one month at 5 • C, 25 • C, and 40 • C. Furthermore, the cAuNPs activity to modulate early marker of fibrosis was tested on NIH/3T3 cells. The optimum condition for cAuNPs synthesis was by using 1.5 mM curcumin at pH 9.3. As compared to free curcumin, cAuNPs showed higher antioxidant activity and maintained the nanosize after stored for one month. In line with the antioxidant activity, cAuNPs 0.25-1 µg/mL reduced the collagen production by NIH/3T3 cells. More importantly, cAuNPs did not demonstrate any effect on the development of chicken embryo. Taken together, the attachment of gold to curcumin in the form of cAuNPs is promising for curcumin targeting to treat hepatic fibrosis.

show abstract

Section: Introductionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 66%

See 1 more Smart Citation

Antifibrotic Activity and In Ovo Toxicity Study of Liver-Targeted Curcumin-Gold Nanoparticle

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…12 Another promising anti-hepatic fibrosis agent is curcumin (Cur), a major curcuminoid from turmeric. 8,18,19 Cur effectively mitigates hepatic fibrosis by inhibiting HSC activation through multiple pathways: blocking leptin and succinate signaling, regulating intracellular glucose and its derivatives, modulating lipid metabolism of HSCs, regulating matrix metalloproteinases (MMPs), and activating peroxisome proliferator-activated receptors (PPARγ) signaling. [20][21][22] We reasoned that Cyc and Cur might inhibit HSC activation synergistically, thereby slowing or even reversing hepatic fibrosis.…”

Section: Introductionmentioning

confidence: 99%

Curcumin- and Cyclopamine-Loaded Liposomes to Enhance Therapeutic Efficacy Against Hepatic Fibrosis

Zhang

Song

et al. 2020

DDDT

View full text Add to dashboard Cite

Background and Purpose Hepatic fibrosis is a public health problem characterized by activation of hepatic stellate cells (HSCs), which triggers excessive production of extracellular matrix (ECM). Inhibition of HSC activation may be an effective treatment. Since various pathways control HSC activation, a combination of drugs with different mechanisms may be more effective than monotherapy. Methods Here, we prepared liposomes loaded with curcumin and cyclopamine to inhibit HSC activation. We systematically analyzed the physicochemical characteristics of liposomes loaded with the two drugs, as well as their effects on HSC proliferation, activation and collagen production on gene, protein and cellular levels. Results The prepared liposomes helped solubilize both drugs, contributing to their uptake by cells. Liposomes loaded with both drugs inhibited cell proliferation, migration and invasion, as well as induced more apoptosis and perturbed the cell cycle more than the free combination of both drugs in solution or liposomes loaded with either drug alone. Liposomes loaded with both drugs strongly suppressed HSC activation and collagen secretion. Conclusion Our results suggest that liposome encapsulation can increase the uptake of curcumin and cyclopamine as well as the synergism between them in anti-fibrosis. This approach shows potential for treating hepatic fibrosis.

show abstract

“…In addition to poor solubility and permeability, the low bioavailability of curcumin is also worsened by intensive hepatic metabolism to more hydrophilic substances which are inactive [ 2 ]. In relation to that study, we also did an in vitro assay of challenging curcumin in liver homogenate and observed rapid degradation after incubation at 37 °C [ 3 ]. Curcumin is reported as a potent anti-inflammatory agent [ 4 , 5 , 6 , 7 ]; however, its complex problems led to clinical failure when this compound was used.…”

Section: Introductionmentioning

confidence: 99%

Multiple Functions of D-α-Tocopherol Polyethylene Glycol 1000 Succinate (TPGS) as Curcumin Nanoparticle Stabilizer: In Vivo Kinetic Profile and Anti-Ulcerative Colitis Analysis in Animal Model

et al. 2017

Self Cite

View full text Add to dashboard Cite

This study was conducted to evaluate the potential benefit of particle reduction down to nanoscale on curcumin, a unique natural active compound facing therapeutic problems due to low solubility and permeability. In addition, the presence of TPGS as a surfactant for multiple functions on curcumin nanoparticle was addressed. Observation was focused on bioavailability enhancement after oral administration and local anti-inflammatory improvement after rectal dosing. Nanonization of curcumin was performed using an up-scalable top down method. Specific animal models were used to study the in vivo kinetic profile and the biological activity of curcumin nanoparticle, compared with curcumin powder. d-α-tocopherol polyethylene glycol 1000 succinate (TPGS)-stabilized curcumin nanoparticle was prepared through homogenization with high pressure of the 1500 bar. An in vivo study was performed after oral administration of the preparations to male healthy Wistar rats, to monitor the plasma kinetic profile of curcumin. The biological activity study was conducted after rectal administration of the preparations in Wistar rats induced by 2,4,6-trinitrobenzene sulfonic acid to develop ulcerative colitis. The curcumin nanoparticle with a size of approximately 200 nm was successfully produced and revealed a better in vivo kinetic profile over the larger size of curcumin mixed with TPGS, with bioavailability (AUC0-∞) that was accounted for seven-fold. In addition, the TPGS-stabilized curcumin nanoparticle demonstrated a superior local anti-inflammatory effect in ulcerative colitis, indicated by the shifting of observed parameters close to the healthy status. The tremendously improved anti-inflammatory effect of the TPGS-stabilized curcumin nanoparticle was found with a very low dose. Reducing the particle size of curcumin down to ~200 nm with the presence of TPGS seems to be a promising approach to improving the therapeutic value of curcumin.

show abstract

Co-delivery of curcumin-loaded nanoemulsion and Phaleria macrocarpa extract to NIH 3T3 cell for antifibrosis

Cited by 14 publications

References 26 publications

Antifibrotic Activity and In Ovo Toxicity Study of Liver-Targeted Curcumin-Gold Nanoparticle

Antifibrotic Activity and In Ovo Toxicity Study of Liver-Targeted Curcumin-Gold Nanoparticle

Curcumin- and Cyclopamine-Loaded Liposomes to Enhance Therapeutic Efficacy Against Hepatic Fibrosis

Multiple Functions of D-α-Tocopherol Polyethylene Glycol 1000 Succinate (TPGS) as Curcumin Nanoparticle Stabilizer: In Vivo Kinetic Profile and Anti-Ulcerative Colitis Analysis in Animal Model

Contact Info

Product

Resources

About