Co-delivery of antigens and immunostimulants<i>via</i>a polymersome for improvement of antigen-specific immune response

Lim, Jong Woo; Na, Woonsung; Kim, Hyun Ouk; Yeom, Minjoo; Kang, Aram; Park, Geunseon; Park, Chaewon; Ki, Jisun; Lee, Sojeong; Jung, Bud; Jeong, Hyoung Hwa; Park, Dae‐Won; Song, Daesub; Haam, Seungjoo

doi:10.1039/d0tb00892c

Cited by 22 publications

(20 citation statements)

References 36 publications

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“…Cryogenic transmission electron microscopy (Cryo-TEM) analysis demonstrated that the bilayered nature of the polymersomes remained intact after the conjugation of both compounds (Figure S6a), and DLS measurements confirmed the formation of reproducibly sized particles (Figure S6b). Interestingly, none of the chemical modifications included in the present study did significantly affect the size or morphology of the polymersomes, underscoring their robustness and versality and making them a highly suitable nanocarrier. ,,− …”

Section: Resultsmentioning

confidence: 73%

“…Interestingly, none of the chemical modifications included in the present study did significantly affect the size or morphology of the polymersomes, underscoring their robustness and versality and making them a highly suitable nanocarrier. 45 , 46 , 59 − 61 …”

Section: Resultsmentioning

confidence: 99%

“…The added value of this approach has, for example, already been demonstrated by the coupling of monophosphoryl lipid A and imiquimod onto polymersomes, which resulted in the induction of immune responses against a co-delivered antigen. 45 − 47 In the present study, we investigated the potency and mechanism of Fc fragment- and CpG-mediated bimodal targeting in the context of polymersomes providing evidence that the simultaneous surface decoration of polymersomes with Fc fragments and CpG allows for efficient cell targeting and immune induction. This shows the potential of polymersomes as a versatile delivery system for the development of highly defined nanoparticle-based vaccines.…”

Section: Introductionmentioning

confidence: 99%

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Bimodal Targeting of Human Leukocytes by Fc- and CpG-Decorated Polymersomes to Tune Immune Induction

et al. 2021

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The use of well-defined nanovesicles composed of amphiphilic block copolymers (polymersomes) for delivery of adjuvants and antigens is a promising strategy for vaccine development. However, the potency of nanoparticle vaccines depends on efficient interaction with and activation of cells involved in antigen presentation, which can be achieved by targeting cellular receptors. Here, we showed that the Fc fragment display on the polymersome surface resulted in markedly improved interactions with granulocytes, monocytes, and NK cells, while for “naked” polymersomes, virtually no binding to leukocytes was observed. Moreover, CpG-decorated polymersomes were found to also interact with T and/or B cells. Interestingly, whole blood stimulations with Fc fragment and CpG-decorated polymersomes induced interleukin (IL)-6, IL-8, and TNF-α production, while naked polymersomes did not induce any cytokine production. In conclusion, specific immune induction by polymersomes can be controlled using bimodal targeting of different immune receptors, which is an essential feature for targeted vaccine delivery.

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Section: Resultsmentioning

confidence: 73%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Bimodal Targeting of Human Leukocytes by Fc- and CpG-Decorated Polymersomes to Tune Immune Induction

et al. 2021

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“…The results demonstrate that with the Qβ formulation, Qβ-NiNTA:His-OVA outperformed the simple admixture of Qβ + OVA in terms of antibody production against the target protein OVA. This may be explained by the fact that OVA is being co-delivered with Qβ in the Qβ-NiNTA:His-OVA formulation, therefore achieving co-delivery of antigen and adjuvant to the same cell. − …”

Section: Resultsmentioning

confidence: 99%

Development of a Modular NTA:His Tag Viral Vaccine for Co-delivery of Antigen and Adjuvant

2023

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The SARS-CoV-2 pandemic has highlighted the need for vaccines that are effective, but quickly produced. Of note, vaccines with plug-and-play capabilities that codeliver antigen and adjuvant to the same cell have shown remarkable success. Our approach of utilizing a nitrilotriacetic acid (NTA) histidine (His)-tag chemistry with viral adjuvants incorporates both of these characteristics: plug-and-play and co-delivery. We specifically utilize the cowpea mosaic virus (CPMV) and the virus-like particles from bacteriophage Qβ as adjuvants and bind the model antigen ovalbumin (OVA). Successful binding of the antigen to the adjuvant/carrier was verified by SDS-PAGE, western blot, and ELISA. Immunization in C57BL/6J mice demonstrates that with Qβ -but not CPMV -there is an improved antibody response against the target antigen using the Qβ-NiNTA:His-OVA versus a simple admixture of antigen and adjuvant. Antibody isotyping also shows that formulation of the vaccines can alter T helper biases; while the Qβ-NiNTA:His-OVA particle produces a balanced Th1/Th2 bias the admixture was strongly Th2. In a mouse model of B16F10-OVA, we further demonstrate improved survival and slower tumor growth in the vaccine groups compared to controls. The NiNTA:His chemistry demonstrates potential for rapid development of future generation vaccines enabling plug-and-play capabilities with effectiveness boosted by co-delivery to the same cell.

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“…However, the type of association method (conjugation or encapsulation) may affect the extent of immune responses [121]. The co-encapsulation of antigens and MPLA in polymersomes significantly increased antigen-specific antibody production and cytokine expression levels compared to those achieved via the association of antigens and MPLA without vehicles [123]. Associating antigens with nanoparticulate systems may also promote immune responses mediated by T helper or cytotoxic T lymphocytes.…”

Section: Polymeric Npsmentioning

confidence: 99%

Application of Nanomaterials as an Advanced Strategy for the Diagnosis, Prevention, and Treatment of Viral Diseases

et al. 2021

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The coronavirus disease (COVID-19) pandemic poses serious global health concerns with the continued emergence of new variants. The periodic outbreak of novel emerging and re-emerging infectious pathogens has elevated concerns and challenges for the future. To develop mitigation strategies against infectious diseases, nano-based approaches are being increasingly applied in diagnostic systems, prophylactic vaccines, and therapeutics. This review presents the properties of various nanoplatforms and discusses their role in the development of sensors, vectors, delivery agents, intrinsic immunostimulants, and viral inhibitors. Advanced nanomedical applications for infectious diseases have been highlighted. Moreover, physicochemical properties that confer physiological advantages and contribute to the control and inhibition of infectious diseases have been discussed. Safety concerns limit the commercial production and clinical use of these technologies in humans; however, overcoming these limitations may enable the use of nanomaterials to resolve current infection control issues via application of nanomaterials as a platform for the diagnosis, prevention, and treatment of viral diseases.

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Co-delivery of antigens and immunostimulantsviaa polymersome for improvement of antigen-specific immune response

Abstract:
Bilayer spherical polymersome based adjuvants promote the antigen cellular uptake into antigen-presenting cells. The administration of polymersome loading OVA and MPLA induce the secretion of cytokines by macrophage activation and elicit potent antigen-specific antibody responses.

Cited by 22 publications

References 36 publications

Bimodal Targeting of Human Leukocytes by Fc- and CpG-Decorated Polymersomes to Tune Immune Induction

Bimodal Targeting of Human Leukocytes by Fc- and CpG-Decorated Polymersomes to Tune Immune Induction

Development of a Modular NTA:His Tag Viral Vaccine for Co-delivery of Antigen and Adjuvant

Application of Nanomaterials as an Advanced Strategy for the Diagnosis, Prevention, and Treatment of Viral Diseases

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Co-delivery of antigens and immunostimulantsviaa polymersome for improvement of antigen-specific immune response

Abstract: Bilayer spherical polymersome based adjuvants promote the antigen cellular uptake into antigen-presenting cells. The administration of polymersome loading OVA and MPLA induce the secretion of cytokines by macrophage activation and elicit potent antigen-specific antibody responses.

Cited by 22 publications

References 36 publications

Bimodal Targeting of Human Leukocytes by Fc- and CpG-Decorated Polymersomes to Tune Immune Induction

Bimodal Targeting of Human Leukocytes by Fc- and CpG-Decorated Polymersomes to Tune Immune Induction

Development of a Modular NTA:His Tag Viral Vaccine for Co-delivery of Antigen and Adjuvant

Application of Nanomaterials as an Advanced Strategy for the Diagnosis, Prevention, and Treatment of Viral Diseases

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Product

Resources

About

Abstract:
Bilayer spherical polymersome based adjuvants promote the antigen cellular uptake into antigen-presenting cells. The administration of polymersome loading OVA and MPLA induce the secretion of cytokines by macrophage activation and elicit potent antigen-specific antibody responses.