Co-culturing microbial consortia: approaches for applications in biomanufacturing and bioprocessing

Kapoore, Rahul Vijay; Padmaperuma, Gloria; Maneein, Supattra; Vaidyanathan, Seetharaman

doi:10.1080/07388551.2021.1921691

Cited by 42 publications

(16 citation statements)

References 159 publications

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“…The study of cave and fumarole microbial communities is also important to questions in biotechnology, sustainable resource management, and bioremediation (Brune and Bayer, 2012 ; Zhang et al, 2016 ; Kapoore et al, 2022 ). Bioleaching organisms are commonly used in mining, and previous research has discovered that the recovery of target metals is often greater with co-cultures of chemolithotrophic organisms (Mathew and Krishnaswamy, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Islands Within Islands: Bacterial Phylogenetic Structure and Consortia in Hawaiian Lava Caves and Fumaroles

et al. 2022

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Lava caves, tubes, and fumaroles in Hawai‘i present a range of volcanic, oligotrophic environments from different lava flows and host unexpectedly high levels of bacterial diversity. These features provide an opportunity to study the ecological drivers that structure bacterial community diversity and assemblies in volcanic ecosystems and compare the older, more stable environments of lava tubes, to the more variable and extreme conditions of younger, geothermally active caves and fumaroles. Using 16S rRNA amplicon-based sequencing methods, we investigated the phylogenetic distinctness and diversity and identified microbial interactions and consortia through co-occurrence networks in 70 samples from lava tubes, geothermal lava caves, and fumaroles on the island of Hawai‘i. Our data illustrate that lava caves and geothermal sites harbor unique microbial communities, with very little overlap between caves or sites. We also found that older lava tubes (500–800 yrs old) hosted greater phylogenetic diversity (Faith's PD) than sites that were either geothermally active or younger (<400 yrs old). Geothermally active sites had a greater number of interactions and complexity than lava tubes. Average phylogenetic distinctness, a measure of the phylogenetic relatedness of a community, was higher than would be expected if communities were structured at random. This suggests that bacterial communities of Hawaiian volcanic environments are phylogenetically over-dispersed and that competitive exclusion is the main driver in structuring these communities. This was supported by network analyses that found that taxa (Class level) co-occurred with more distantly related organisms than close relatives, particularly in geothermal sites. Network “hubs” (taxa of potentially higher ecological importance) were not the most abundant taxa in either geothermal sites or lava tubes and were identified as unknown families or genera of the phyla, Chloroflexi and Acidobacteria. These results highlight the need for further study on the ecological role of microbes in caves through targeted culturing methods, metagenomics, and long-read sequence technologies.

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Section: Discussionmentioning

confidence: 99%

Islands Within Islands: Bacterial Phylogenetic Structure and Consortia in Hawaiian Lava Caves and Fumaroles

et al. 2022

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“…A significant challenge in the formulation process of microorganisms’ consortia is to untangle the interactions among the strains that compose the consortium. Evaluating the species interaction in a consortium and deciphering the dynamics within the establishment of coexistence in microbial-based product design is a complex task [ 54 ]. Although microorganisms live in nature within complex ecological communities [ 55 , 56 , 57 ], assembling a synthetic microbial consortium [ 58 ] within a growth substrate can provoke unexpected antagonistic or not beneficial behaviors that compromise the bio-production process [ 59 ].…”

Section: Methods For the Study Of Bioinocula Interactions With Soil M...mentioning

confidence: 99%

Microbial-Based Products to Control Soil-Borne Pathogens: Methods to Improve Efficacy and to Assess Impacts on Microbiome

Ptaszek

Canfora²,

Pugliese

et al. 2023

Microorganisms

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Microbial-based products (either as biopesticide or biofertilizers) have a long history of application, though their use is still limited, mainly due to a perceived low and inconsistent efficacy under field conditions. However, their efficacy has always been compared to chemical products, which have a completely different mechanism of action and production process, following the chemical paradigm of agricultural production. This paradigm has also been applied to regulatory processes, particularly for biopesticides, making the marketing of microbial-based formulations difficult. Increased knowledge about bioinocula behavior after application to the soil and their impact on soil microbiome should foster better exploitation of microbial-based products in a complex environment such as the soil. Moreover, the multifunctional capacity of microbial strains with regard to plant growth promotion and protection should also be considered in this respect. Therefore, the methods utilized for these studies are key to improving the knowledge and understanding of microbial-based product activity and improving their efficacy, which, from farmers’ point of view, is the parameter to assess the usefulness of a treatment. In this review, we are thus addressing aspects related to the production and formulation process, highlighting the methods that can be used to evaluate the functioning and impact of microbial-based products on soil microbiome, as tools supporting their use and marketing.

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“…The cocultivation group also derived a superior AA profile (Alloul et al 2021b). Moreover, mixed cultures have also been reported to enhance pelletization, flocculation, and biofilm formation (Kapoore et al 2022).…”

Section: Pnsb Strainmentioning

confidence: 99%

Single-cell protein production from purple non-sulphur bacteria-based wastewater treatment

Wada

Vincent

Mackey

2022

Rev Environ Sci Biotechnol

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Large-scale production of single-cell protein (SCP) has the potential not only to solve some of the food insecurity and water scarcity crises that plague a significant portion of our world today but also holds the promise to reduce the cost associated with the treatment of industrial and agricultural wastewater. Resource recovery of SCP from organic waste by microbes like yeast and microalgae is commonly documented. However, recently, a class of phototrophic bacteria, purple non-sulphur bacteria (PNSB), has emerged as a favourable option in terms of both wastewater treatment and resource recovery. PNSB are metabolically versatile and tolerant to a wide range of conditions, hence their ability to thrive in diverse waste streams. Besides its rich protein content, PNSB contains other nutritionally valuable bioproducts like carotenoids, coenzyme Q10, 5-aminolevulinic acid, and pantothenic acid. Recent evidence also indicates that PNSB-based aquafeed enhances growth and boosts immunity in certain aquaculture trials. It does not possess the same toxicity as most gram-negative bacteria due to its comparatively less potent lipopolysaccharide composition. With diverse promising prospects of PNSB-based SCP, it is critical to extensively examine the landscape from a holistic standpoint, highlighting the potential challenges large-scale SCP production may pose. Thus, this review explores the comparative advantages of utilizing PNSB for SCP production, essential components of PNSB-based SCP processing, and possible environmental and economic gains associated with the process. Current challenges with PNSB-based SCP production and future outlooks are also examined.

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Co-culturing microbial consortia: approaches for applications in biomanufacturing and bioprocessing

Cited by 42 publications

References 159 publications

Islands Within Islands: Bacterial Phylogenetic Structure and Consortia in Hawaiian Lava Caves and Fumaroles

Islands Within Islands: Bacterial Phylogenetic Structure and Consortia in Hawaiian Lava Caves and Fumaroles

Microbial-Based Products to Control Soil-Borne Pathogens: Methods to Improve Efficacy and to Assess Impacts on Microbiome

Single-cell protein production from purple non-sulphur bacteria-based wastewater treatment

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