Co-cultured spheroids of human periodontal ligament mesenchymal stem cells and vascular endothelial cells enhance periodontal tissue regeneration

Sano, Koichi; Usui, Michihiko; Moritani, Yuki; Nakazawa, Kimitaka; Hanatani, Tomoya; Kondo, Hisataka; Nakatomi, Mitsushiro; Onizuka, Satoru; Iwata, Takanori; Sato, Tsuyoshi; Togari, Akifumi; Ariyoshi, Wataru; Nishihara, Tatsuji; Nakashima, Keisuke

doi:10.1016/j.reth.2019.12.008

Cited by 32 publications

(28 citation statements)

References 42 publications

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“…tissue with dimensions greater than 100-200 microns in order to allow optimal oxygen and nutrient supply. [96,97] Their reorganization within the 3D systems should prime for the formation of neo-vessels. As described previously, mechanoreceptors are also the integrative component of PDL anatomy and are crucial in transforming mechanical information into biochemical signals during OTM.…”

Section: D In Vitro Models To Study Regenerative Potential Of Bioengmentioning

confidence: 99%

Current Trends in In Vitro Modeling to Mimic Cellular Crosstalk in Periodontal Tissue

Aveic

Craveiro

Wolf

et al. 2020

Adv Healthcare Materials

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Clinical evidence indicates that in physiological and therapeutic conditions a continuous remodeling of the tooth root cementum and the periodontal apparatus is required to maintain tissue strength, to prevent damage, and to secure teeth anchorage. Within the tooth's surrounding tissues, tooth root cementum and the periodontal ligament are the key regulators of a functional tissue homeostasis. While the root cementum anchors the periodontal fibers to the tooth root, the periodontal ligament itself is the key regulator of tissue resorption, the remodeling process, and mechanical signal transduction. Thus, a balanced crosstalk of both tissues is mandatory for maintaining the homeostasis of this complex system. However, the mechanobiological mechanisms that shape the remodeling process and the interaction between the tissues are largely unknown. In recent years, numerous 2D and 3D in vitro models have sought to mimic the physiological and pathophysiological conditions of periodontal tissue. They have been proposed to unravel the underlying nature of the cell–cell and the cell–extracellular matrix interactions. The present review provides an overview of recent in vitro models and relevant biomaterials used to enhance the understanding of periodontal crosstalk and aims to provide a scientific basis for advanced regenerative strategies.

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Section: D In Vitro Models To Study Regenerative Potential Of Bioengmentioning

confidence: 99%

Current Trends in In Vitro Modeling to Mimic Cellular Crosstalk in Periodontal Tissue

Aveic

Craveiro

Wolf

et al. 2020

Adv Healthcare Materials

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“…As populations age, periodontitis causing tooth loss has increased 101 . Spheroids consisting of a mixture of human periodontal ligament MSCs and HUVECs may reduce periodontitis 102 . Spheroid transplantation into rat periodontal tissue defects shows increased bone and cementum formation to a greater extent than did spheroids with only one cell type.…”

Section: Spheroid Applications In Tementioning

confidence: 99%

The utility of biomedical scaffolds laden with spheroids in various tissue engineering applications

et al. 2021

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A spheroid is a complex, spherical cellular aggregate supporting cell-cell and cell-matrix interactions in an environment that mimics the real-world situation. In terms of tissue engineering, spheroids are important building blocks that replace two-dimensional cell cultures. Spheroids replicate tissue physiological activities. The use of spheroids with/without scaffolds yields structures that engage in desired activities and replicate the complicated geometry of three-dimensional tissues. In this mini-review, we describe conventional and novel methods by which scaffold-free and scaffolded spheroids may be fabricated and discuss their applications in tissue regeneration and future perspectives.

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“…When cultured as 3D spheroids, DFSCs had an enhanced osteogenic potential in vitro [411]. Sano and colleagues investigated the potential of co-cultured spheroids of PDLSCs and HUVECs for periodontal tissue regeneration in vitro and in vivo [412]. When PLDSCs and HUVECs spheroids were cultured in a ratio of 1:2, the expression of OCT4 and NANOG was increased.…”

Section: Hard Tissue Regeneration Approached By Dental Stem Cellsmentioning

confidence: 99%

Sinking Our Teeth in Getting Dental Stem Cells to Clinics for Bone Regeneration

Shoushrah

Transfeld

Tonk

et al. 2021

IJMS

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Dental stem cells have been isolated from the medical waste of various dental tissues. They have been characterized by numerous markers, which are evaluated herein and differentiated into multiple cell types. They can also be used to generate cell lines and iPSCs for long-term in vitro research. Methods for utilizing these stem cells including cellular systems such as organoids or cell sheets, cell-free systems such as exosomes, and scaffold-based approaches with and without drug release concepts are reported in this review and presented with new pictures for clarification. These in vitro applications can be deployed in disease modeling and subsequent pharmaceutical research and also pave the way for tissue regeneration. The main focus herein is on the potential of dental stem cells for hard tissue regeneration, especially bone, by evaluating their potential for osteogenesis and angiogenesis, and the regulation of these two processes by growth factors and environmental stimulators. Current in vitro and in vivo publications show numerous benefits of using dental stem cells for research purposes and hard tissue regeneration. However, only a few clinical trials currently exist. The goal of this review is to pinpoint this imbalance and encourage scientists to pick up this research and proceed one step further to translation.

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Co-cultured spheroids of human periodontal ligament mesenchymal stem cells and vascular endothelial cells enhance periodontal tissue regeneration

Cited by 32 publications

References 42 publications

Current Trends in In Vitro Modeling to Mimic Cellular Crosstalk in Periodontal Tissue

Current Trends in In Vitro Modeling to Mimic Cellular Crosstalk in Periodontal Tissue

The utility of biomedical scaffolds laden with spheroids in various tissue engineering applications

Sinking Our Teeth in Getting Dental Stem Cells to Clinics for Bone Regeneration

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