Co-Culture Systems for Vasculogenesis

Perry, Luba; Ben-Shaul, Shahar; Landau, Shira; Levenberg, Shulamit

doi:10.1007/978-3-319-21056-8_7-1

Cited by 4 publications

(5 citation statements)

References 131 publications

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“…15,43,44 Among those variety of primary cells, HUVECs are predominantly used for tissue engineering studies for both large and small vasculature development, since they preserve nearly all the native vascular endothelium features. 13,58 They have been already investigated within microchannels for transendothelial studies after reaching confluency. 59 In addition, Mori et al developed an interesting system of perfusable channels to supplement a skin-equivalent graft by seeding HUVECs in channels made of collagen and with a width of about 500 μm.…”

Section: Discussionmentioning

confidence: 99%

“…Different sources of primary endothelial cells can be used for in vitro experiments, such as human dermal microvascular endothelial cells (HDMVECs), human coronary artery endothelial cells (HCECs), human aortic endothelial cells (HAECs), human pulmonary artery endothelial cells (HPAECs), and human umbilical vein endothelial cells (HUVECs). ,, Among those variety of primary cells, HUVECs are predominantly used for tissue engineering studies for both large and small vasculature development, since they preserve nearly all the native vascular endothelium features. , They have been already investigated within microchannels for transendothelial studies after reaching confluency . In addition, Mori et al developed an interesting system of perfusable channels to supplement a skin-equivalent graft by seeding HUVECs in channels made of collagen and with a width of about 500 μm .…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, they preserve nearly all the native-vascular endothelium features found in vivo in physiological conditions. 13 In addition, HUVECs have been used in combination with other cell lines, peptides and other biological factors to mimic the in vivo conditions in which cells are surrounded by numerous soluble factors, extracellular matrix, and supporting cells such as pericytes. 6 A detailed description of the cellular and molecular mechanisms of vascular lumen (tubular structure) formation was reported by Iruela-Arispe et al 14 Briefly, cord hollowing and cell hollowing are the two main mechanisms of lumen formation for endothelial cells.…”

Section: Introductionmentioning

confidence: 99%

“…Human umbilical vein endothelial cells (HUVECs) are widely used as optimal cell lineage candidates for the in vitro formation of capillary networks in engineered tissue grafts. Indeed, they preserve nearly all the native-vascular endothelium features found in vivo in physiological conditions . In addition, HUVECs have been used in combination with other cell lines, peptides and other biological factors to mimic the in vivo conditions in which cells are surrounded by numerous soluble factors, extracellular matrix, and supporting cells such as pericytes .…”

Section: Introductionmentioning

confidence: 99%

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Microchannel Molding Combined with Layer-by-Layer Approach for the Formation of Three-Dimensional Tube-like Structures by Endothelial Cells

Aor

Khan

Glinel

et al. 2020

ACS Appl. Bio Mater.

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The development of a functional in vitro model for microcirculation is an unresolved challenge, with major impact for the creation and regeneration of organs in the tissue engineering. The absence of prevascularized engineered tissues limits enormously their efficacy and integration. Therefore, in this study, the in vitro formation of tubular-like structures with human umbilical vein endothelial cells (HUVECs) is investigated thanks to three-dimensional polycarbonate (PC) microchannel (μCh) scaffolds, surface biofunctionalized with hyaluronic acid/chitosan (HA/CHI) layer-by-layer (LbL) films grafted with adhesive (RGD) and angiogenic (SVV and QK) peptides, alone and in combination. The importance of this work lies in the formation of capillaries in the order of tens of μm, developing spontaneous microvessels, without the complexity of microfluidic approaches, and in a short time-scale. Ellipsometry, confocal laser scanning microscopy, and fluorospectrometry are used to characterize the biofunctionalized microchannels. PC-μCh scaffolds functionalized with (HA/CHI)12.5 film (PC-LbL) and further grafted with RGD and QK peptides (PC-RGD+QK) or with RGD and SVV peptides (PC-RGD+SVV) are then tested for in vitro blood vessel formation. These assays evidence a rapid formation of tubular-like structures after 2 h of incubation. Moreover, a coculture system involving HUVECs and human pericytes derived from placenta (hPCs-PL) stabilizes the tubes for a longer time.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Microchannel Molding Combined with Layer-by-Layer Approach for the Formation of Three-Dimensional Tube-like Structures by Endothelial Cells

Aor

Khan

Glinel

et al. 2020

ACS Appl. Bio Mater.

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“…While much progress has been made in the field of tissue engineering, most studies utilize immunocompromised animal models or administer immunosuppressive therapy to study the integration and survival of human engineered tissues in animals [8][9][10][11][12][13][14]. These works have established that in vitro graft prevascularization by human vascular cells is beneficial for graft integration and neovascularization post-implantation [8][9][10]13,[15][16][17]. It was also shown that culturing human vascular cells on biodegradable and biocompatible poly (l-lactic acid)/poly (lactide-co-glycolic acid) (PLLA/PLGA) scaffolds combined with fibrin gel results in the spontaneous formation of vessel-like structures in vitro, which later anastomose with the host vasculature upon transplantation into immunocompromised animals [9,16,18].…”

Section: Introductionmentioning

confidence: 99%

Enhanced Host Neovascularization of Prevascularized Engineered Muscle Following Transplantation into Immunocompetent versus Immunocompromised Mice

Perry

Merdler

Elishaev

et al. 2019

Cells

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Engineering of functional tissue, by combining either autologous or allogeneic cells with biomaterials, holds promise for the treatment of various diseases and injuries. Prevascularization of the engineered tissue was shown to enhance and improve graft integration and neovascularization post-implantation in immunocompromised mice. However, the neovascularization and integration processes of transplanted engineered tissues have not been widely studied in immunocompetent models. Here, we fabricated a three-dimensional (3D) vascularized murine muscle construct that was transplanted into immunocompetent and immunocompromised mice. Intravital imaging demonstrated enhanced neovascularization in immunocompetent mice compared to immunocompromised mice, 18 days post-implantation, indicating the advantageous effect of an intact immune system on neovascularization. Moreover, construct prevascularization enhanced neovascularization, integration, and myogenesis in both animal models. These findings demonstrate the superiority of implantation into immunocompetent over immunocompromised mice and, therefore, suggest that using autologous cells might be beneficial compared to allogeneic cells and subsequent immunosuppression. Taken together, these observations have the potential to advance the field of regenerative medicine and tissue engineering, ultimately reducing the need for donor organs and tissues.

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Genetically engineered human muscle transplant enhances murine host neovascularization and myogenesis

et al. 2018

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Engineered tissues are a promising tool for addressing the growing need for tissues and organs in surgical reconstructions. Prevascularization of implanted tissues is expected to enhance survival prospects post transplantation and minimize deficiencies and/or hypoxia deeper in the tissue. Here, we fabricate a three-dimensional, prevascularized engineered muscle containing human myoblasts, genetically modified endothelial cells secreting angiopoietin 1 (ANGPT1) and genetically modified smooth muscle cells secreting vascular endothelial growth factor (VEGF). The genetically engineered human muscle shows enhanced host neovascularization and myogenesis following transplantation into a mouse host, compared to the non-secreting control. The vascular, genetically modified cells have been cleared for clinical trials and can be used to construct autologous vascularized tissues. Therefore, the described genetically engineered vascularized muscle has the potential to be fully translated to the clinical setting to overcome autologous tissue shortage and to accelerate host neovascularization and integration of engineered grafts following transplantation.

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Co-Culture Systems for Vasculogenesis

Cited by 4 publications

References 131 publications

Microchannel Molding Combined with Layer-by-Layer Approach for the Formation of Three-Dimensional Tube-like Structures by Endothelial Cells

Microchannel Molding Combined with Layer-by-Layer Approach for the Formation of Three-Dimensional Tube-like Structures by Endothelial Cells

Enhanced Host Neovascularization of Prevascularized Engineered Muscle Following Transplantation into Immunocompetent versus Immunocompromised Mice

Genetically engineered human muscle transplant enhances murine host neovascularization and myogenesis

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