Co-culture: stimulate the metabolic potential and explore the molecular diversity of natural products from microorganisms

Peng, Xiao-Yue; Wu, Jintao; Shao, Chang‐Lun; Li, Zhiyong; Chen, Min; Wang, Chang‐Yun

doi:10.1007/s42995-020-00077-5

Cited by 52 publications

(26 citation statements)

References 50 publications

(50 reference statements)

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“…Therefore, cocultures represent an efficient strategy that can be exploited for lipid and biomass production. Coculturing fungi and bacteria better emulates their natural living environment and may enable the activation of silent genes or gene clusters in both organisms through interspecies interactions 26 . The coculturing of different microorganisms often improves their production of various compounds and can lead to the production of cryptic compounds not detected in monocultures under various culturing conditions 27 .…”

Section: Discussionmentioning

confidence: 99%

Coculturing of Mucor plumbeus and Bacillus subtilis bacterium as an efficient fermentation strategy to enhance fungal lipid and gamma-linolenic acid (GLA) production

Mohamed

Awad

Shah

et al. 2022

Sci Rep

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This study aimed to improve lipid and gamma-linolenic acid (GLA) production of an oleaginous fungus, Mucor plumbeus, through coculturing with Bacillus subtilis bacteria, optimising the environmental and nutritional culture conditions, and scaling them for batch fermentation. The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes were 14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%, respectively. This strategy uses cellobiose in place of glucose, decreasing production costs. The nutritional and abiotic factor results suggest that the highest production efficiency is achieved at 6.5 pH, 30 °C temperature, 10% (v/v) inoculum composition, 200 rpm agitation speed, and a 5-day incubation period. Interestingly, the GLA concentration of cocultures (20.0 ± 0.5%) was twofold higher than that of monocultures (8.27 ± 0.11%). More importantly, the GC chromatograms of cocultures indicated the presence of one additional peak corresponding to decanoic acid (5.32 ± 0.20%) that is absent in monocultures, indicating activation of silent gene clusters via cocultivation with bacteria. This study is the first to show that coculturing of Mucor plumbeus with Bacillus subtilis is a promising strategy with industrialisation potential for the production of GLA-rich microbial lipids and prospective biosynthesis of new products.

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Section: Discussionmentioning

confidence: 99%

Coculturing of Mucor plumbeus and Bacillus subtilis bacterium as an efficient fermentation strategy to enhance fungal lipid and gamma-linolenic acid (GLA) production

Mohamed

Awad

Shah

et al. 2022

Sci Rep

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“…(Sordariomycetes, Diaporthaceae) [ 43 ]. In two cases, the partner microbe was represented by Streptomyces strains (Actinomycetota), which are well-known for their capacity to modulate the metabolic potential of fungi [ 60 ].…”

Section: Fungal Sourcesmentioning

confidence: 99%

New Insights into Chemical and Biological Properties of Funicone-like Compounds

Salvatore

DellaGreca

Andolfi

et al. 2022

Toxins

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Funicone-like compounds are a homogeneous group of polyketides that, so far, have only been reported as fungal secondary metabolites. In particular, species in the genus Talaromyces seem to be the most typical producers of this group of secondary metabolites. The molecular structure of funicone, the archetype of these products, is characterized by a γ-pyrone ring linked through a ketone group to a α-resorcylic acid nucleus. This review provides an update on the current knowledge on the chemistry of funicone-like compounds, with special emphasis on their classification, occurrence, and diverse biological activities. In addition, their potential relevance as mycotoxins is discussed.

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“…It should be noted that co-culture strategies have proven simple and efficient without the need for complex genetic level operations, expensive reagents and have been widely applied in the field of discovering novel and bioactive microbe-derived natural products [ 12 ]. Co-culture mimicking the natural environment through mixed fermentation of different microorganisms (also called co-cultivation) may lead to an enhancement in the production of compounds [ 13 ] and even trigger the expression of silent biosynthetic pathways [ 14 ], leading to the accumulation of new natural products [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis of the production enhancement mechanism of antimicrobial lipopeptides of Streptomyces bikiniensis HD-087 by co-culture with Magnaporthe oryzae Guy11

et al. 2022

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The lipopeptides produced by Streptomyces bikiniensis have a significant inhibitory effect on Magnaporthe oryzae, but the low yield limits its application. In this study, the anti-M. oryzae activity of the broth of S. bikiniensis HD-087 co-cultured with M. oryzae Guy11 mycelium has risen by 41.22% compared with pure culture, and under induction conditions of adding Guy11-inducer (cell-free supernatant of M. oryzae Guy11), the activity of strain HD-087 improved 61.76%. The result proved that the enhancement effect of Guy11 on the antimicrobial activity of HD-087 was mainly related to metabolites but mycelium cells. Under optimum induction conditions, NRPS gene expression levels of HD-087 were significantly increased by induction with Guy11-inducer, the biomass of HD-087 had no significant change, but crude extract of lipopeptide (CEL) production was 107.4% higher than pure culture, and TLC result under acid hydrolysis showed that the induced culture has one component more than pure culture. To clarify the regulation mechanism of improving lipopeptide production of HD-087 with Guy11-inducer, transcriptomic analysis was performed using RNAseq to compare the induced culture and pure culture. In the induced culture, 943 genes were up-regulated, while 590 genes were down-regulated in DEGs (differentially expressed genes). KEGG results showed that the expression of genes related to amino acid synthesis, fatty acid metabolism, TCA cycle and pyruvate metabolism pathway were significantly increased. The increased expression of genes related to these metabolic pathways provided sufficient precursors for lipopeptide synthesis. Accordingly, key enzyme genes responsible for the synthesis of lipopeptides Srf and NRPS was significantly increased. Quorum sensing related genes OppA and MppA were significantly up-regulated, and then ComP was activated and promoted lipopeptide synthesis. These results provided a scientific basis for using M. oryzae to induce the increase of the production of Streptomyces lipopeptides, and also laid a foundation for further exploring the co-culture mechanisms among different genera.

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Co-culture: stimulate the metabolic potential and explore the molecular diversity of natural products from microorganisms

Cited by 52 publications

References 50 publications

Coculturing of Mucor plumbeus and Bacillus subtilis bacterium as an efficient fermentation strategy to enhance fungal lipid and gamma-linolenic acid (GLA) production

Coculturing of Mucor plumbeus and Bacillus subtilis bacterium as an efficient fermentation strategy to enhance fungal lipid and gamma-linolenic acid (GLA) production

New Insights into Chemical and Biological Properties of Funicone-like Compounds

Transcriptome analysis of the production enhancement mechanism of antimicrobial lipopeptides of Streptomyces bikiniensis HD-087 by co-culture with Magnaporthe oryzae Guy11

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