Co-culture of mesenchymal stem cells and human umbilical vein endothelial cells on heparinized polycaprolactone/gelatin co-spun nanofibers for improved endothelium remodeling

Joshi, Akshat; Xu, Zhongli; Ikegami, Yasuhiro; Yamane, Soichiro; Tsurashima, Masanori; Ijima, Hiroyuki

doi:10.1016/j.ijbiomac.2020.02.163

Cited by 25 publications

(17 citation statements)

References 34 publications

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“…In contrast, when HUVECs and MSCs were cocultured, MSCs were shown to migrate toward HUVECs and supported the formation and maturation of vascular networks [ 2 , 60 ]. Additionally, higher levels of endothelialization were observed in similar studies [ 61 ]. Chen et al used ECs in coculture with hepatocytes.…”

Section: Important Aspects Of Microvascular Tissue Engineeringsupporting

confidence: 62%

Microvascular Tissue Engineering—A Review

et al. 2021

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Tissue engineering and regenerative medicine have come a long way in recent decades, but the lack of functioning vasculature is still a major obstacle preventing the development of thicker, physiologically relevant tissue constructs. A large part of this obstacle lies in the development of the vessels on a microscale—the microvasculature—that are crucial for oxygen and nutrient delivery. In this review, we present the state of the art in the field of microvascular tissue engineering and demonstrate the challenges for future research in various sections of the field. Finally, we illustrate the potential strategies for addressing some of those challenges.

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Section: Important Aspects Of Microvascular Tissue Engineeringsupporting

confidence: 62%

Microvascular Tissue Engineering—A Review

et al. 2021

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“…Material selection is predominantly effective in imitating the physical, chemical, mechanical, and structural properties of the scaffolds 9 . In addition to allowing rapid endothelialization and cell proliferation; biocompatibility, cytocompatibility, slow degradation rate, excellent strength, and high elongation features make PCL an indispensable biopolymer 10‐13 . On the other hand, PLA is an aliphatic bio‐based polymer that is preferable in biomedical applications due to its biocompatibility, rapid biodegradability, lack of toxicity, high mechanical strength, and modulus 13‐15 .…”

Section: Introductionmentioning

confidence: 99%

Development of biodegradable webs of PLA/PCL blends prepared via electrospinning: Morphological, chemical, and thermal characterization

Oztemur

Yalcin-Enis

2021

J Biomed Mater Res

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Biodegradable polymers have a mean role to mimic native tissues and allow cells to penetrate, grow, and proliferate with their advanced features in tissue engineering applications. The physiological, chemical, mechanical, and biological qualities of the surfaces, which are presented from biodegradable polymers, affect the final properties of the scaffolds. In this study, it is aimed to produce fibrous webs by electrospinning method for tissue engineering applications using two different biopolymers, polylactic acid (PLA) and polycaprolactone (PCL). These polymers are used either alone or in a blended form (PLA/PCL, 1/1 wt.). Within the scope of the study, polymer concentrations (6, 8 and 10%) and solvent types (used for chloroform/ethanol/acetic acid mixture, PCL and PLA/PCL mixtures, and chloroform/acetone, PLA) vary as solution parameters. Fibrous webs are investigated in terms of morphological, chemical, and thermal characteristics. Results show continuous fibers are examined for 8 or 10% polymer concentrations with an average fiber diameter of 1.3–2.7 μm and pore area of 4–9 μm2. No fiber formation is observed in sample groups with a polymer concentration of 6% and beaded structures are formed. Water contact angle analysis proves the hydrophobic properties of PLA and PCL, whereas Fourier‐transform infrared results show there is no solution residue on the surfaces, so there is no toxic effect. Also, in differential scanning calorimetry analysis, the characteristic crystallization peaks of the polymers are recognized, and when the polymers are in a blend, it beholds that they have effects on each other's crystallization.

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“…[43] Compelling evidence shows that the adhesion and proliferation of endothelial cells are increased proportionately with the gelatin content in scaffolds [44]. Although numerous studies have explored the artificial blood vessels with coaxial fibers of PCL-gelatin core-shell structure, the mechanical properties of such vessels are insufficient [26,30,45]. Another study spun a layer of pure PCL on the outer layer of the coaxial artificial blood vessel to enhance its mechanical properties, [46] but it was prone to cause vascular stratification.…”

Section: Discussionmentioning

confidence: 99%

Design and characterization of small-diameter tissue-engineered blood vessels constructed by electrospun polyurethane-core and gelatin-shell coaxial fiber

et al. 2021

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Substitution or bypass is the most effective treatment for vascular occlusive diseases.The demand for artificial blood vessels has seen an unprecedented rise due to the limited supply of autologous blood vessels. Tissue engineering is the best approach to provide artificial blood vessels. In this study, a new type of small-diameter artificial blood vessel with good mechanical and biological properties was designed by using electrospinning coaxial fibers. Four groups of coaxial fibers vascular membranes having polyurethane/gelatin core-shell structure were cross-linked by the EDC-NHS system and characterized. The core-shell structure of the coaxial vascular fibers was observed by transmission electron microscope. After the crosslinking, the stress and elastic modulus increased and the elongation decreased, burst pressure of 0.11 group reached the maximum (2844.55 ± 272.65 mmHg) after cross-linking, which acted as the experimental group. Masson staining identified blue-stained ring or elliptical gelatin ingredients in the vascular wall. The cell number in the vascular wall of the coaxial group was found in muscle embedding experiment significantly higher than that of the non-coaxial group at all time points (p < 0.001). Our results showed that the coaxial vascular graft with the ratio of 0.2:0.11 had better mechanical properties (burst pressure reached 2844.55 ± 272.65 mmHg); Meanwhile its biological properties were also outstanding, which was beneficial to cell entry and offered good vascular remodeling performance. Polyurethane (PU); Gelatin (Gel); Polycaprolactone (PCL); polylactic acid (PLA);1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC); N-Hydroxy succinimide (NHS); 4-Morpholine-ethane-sulfonic (MES); phosphate buffered saline (PBS); fetal calf serum (FCS); Minimum Essential Medium (MEM); Dimethyl sulfoxide (DMSO); hematoxylineosin (HE).

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Co-culture of mesenchymal stem cells and human umbilical vein endothelial cells on heparinized polycaprolactone/gelatin co-spun nanofibers for improved endothelium remodeling

Cited by 25 publications

References 34 publications

Microvascular Tissue Engineering—A Review

Microvascular Tissue Engineering—A Review

Development of biodegradable webs of PLA/PCL blends prepared via electrospinning: Morphological, chemical, and thermal characterization

Design and characterization of small-diameter tissue-engineered blood vessels constructed by electrospun polyurethane-core and gelatin-shell coaxial fiber

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