Co‑culture of bone marrow stromal cells and chondrocytes in�vivo for the repair of the goat condylar cartilage defects

Sun, Hao; Huang, Yue; Zhang, Lei; Li, Biao; Wang, Xudong

doi:10.3892/etm.2018.6551

Cited by 7 publications

(35 citation statements)

References 43 publications

(49 reference statements)

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“…Additionally, the TMJs in different species possess different morphologies and sizes of condyles and discs (Kalpakci et al 2011). We chose goats as the animal model because they display notable similarities to humans, including joint anatomy, joint function, and disc size and shape, and they have become the important models of choice for TMJ research (Li et al 2015; Sun et al 2018). Until now, no study has focused on TMJ disc defect and repair on goats.…”

Section: Discussionmentioning

confidence: 99%

3D-Printed Polycaprolactone Reinforced Hydrogel as an Artificial TMJ Disc

et al. 2021

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The replacement of a damaged temporomandibular joint (TMJ) disc remains a long-standing challenge in clinical settings. No study has reported a material with comprehensively excellent properties similar to a natural TMJ disc. In this work, we designed a novel artificial TMJ disc using polyvinyl alcohol (PVA) hydrogel crosslinked by cyclic freeze-thaw and reinforced by 3D-printed polycaprolactone (PCL) implants. The mechanical properties and surface morphologies of the artificial TMJ disc and the natural goat TMJ disc were tested and compared via compression, tensile, cyclic compression/tensile, creep, friction, scanning electron microscopy, and atomic force microscopy. The fibroblasts and chondrocytes were cultured on the artificial TMJ disc for 1, 3, and 5 d for cytotoxicity testing. Importantly, the artificial discs were placed into the TMJs of goats in an innovative way to induce disc defect repair for 12 wk. The PVA + PCL artificial disc demonstrated mechanical strength similar to that of natural disc, as well as 1) better fatigue resistance, viscoelasticity, and hydrophilicity; 2) less creep; and 3) low friction, cytotoxicity, and cell adhesion. By repairing the defects of the TMJ disc in goats, the artificial disc demonstrated the ability to maintain joint stability and protect condylar cartilage and bone from damage. These promising results indicate the feasibility of using a PVA + PCL artificial TMJ disc in a clinical context.

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Section: Discussionmentioning

confidence: 99%

3D-Printed Polycaprolactone Reinforced Hydrogel as an Artificial TMJ Disc

et al. 2021

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“…Apesar de ser mais utilizada em pequenos animais, a aplicação de medula óssea também é encontrada em caprinos, no qual Sun et al (2018) e Wang et al (2018) utilizaram células da medula óssea para regeneração de cartilagem e obtiveram bons resultados.…”

Section: Discussionunclassified

Tibial osteosynthesis with type II external skeletal fixator in a goat

Rodrigues¹,

Araújo²,

Probo³

et al. 2020

Pubvet

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O objetivo deste trabalho foi relatar o caso de um caprino com fratura transversa de tíbia submetido a osteossíntese com fixador esquelético externo tipo II. Uma cabra foi atendida no Hospital Veterinário Adílio Santos de Azevedo do IFPB no campus de Sousa - Paraíba com queixa de claudicação do membro pélvico esquerdo, sendo diagnosticada fratura fechada transversa no terço médio de tíbia. Foi realizada imobilização externa do membro com gesso, porém não houve melhora, sendo necessária a osteossíntese com uso de fixador esquelético externo tipo II. Observou-se o apoio claudicante do membro operado no dia seguinte a cirurgia. Os pontos de pele foram retirados treze dias após o procedimento cirúrgico, pois a ferida estava cicatrizada e sem alterações. O animal ficou internado durante os 25 primeiros dias após a cirurgia, após esse período o animal foi encaminhado de volta a propriedade com a recomendação da limpeza do fixador esquelético externo a cada dois dias e o isolamento do animal até a retirada. Sessenta e oito dias após o procedimento cirúrgico, o animal estava sem apoiar o membro, com secreção purulenta drenando pelo trajeto dos pinos e por ferida penetrante na região lateral do membro pélvico, frouxidão dos pinos e dor a manipulação. O exame radiográfico revelou lise óssea no trajeto dos pinos. O implante foi retirado e prescrito medicação com antibiótico e analgésico. Após vinte dias desse último atendimento, o animal retornou ao HV com as feridas cicatrizadas, sem dor à palpação e apoio intermitente do membro, podendo observar também um desvio no local da fratura devido à má união óssea. Conclui-se que apesar das complicações pós-cirúrgicas, o animal se recuperou da fratura com o uso de fixador esquelético externo tipo II, pois mesmo não havendo a continuidade de manejo pós-cirúrgico adequado, houve união clínica da fratura.

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“…In animal models of TMJ damage, natural collagen (Kobayashi et al, 2015;Wang et al, 2017), synthesized PLGA (Zhu et al, 2011;Dormer et al, 2011), polylactic acid (Ahtiainen et al, 2013), and polycaprolactone (PCL) (Tarafder et al, 2016) were fabricated into scaffolds, whereas Pluronic F-127-based hydrogel supported regenerative cell implantation (Yu et al, 2011;Sun et al, 2018). For cells in the systems, articular chondrocytes, costal chondrocytes, whole bone marrow, bone marrow stem cells (BMSCs), and adipose stem cells (Ahtiainen et al, 2013) were adopted.…”

Section: Novel Delivery Systems For Regenerative Agentsmentioning

confidence: 99%

Therapeutic Agents for the Treatment of Temporomandibular Joint Disorders: Progress and Perspective

Cai

et al. 2021

Front. Pharmacol.

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Temporomandibular joint disorders (TMD) are a common health condition caused by the structural or functional disorders of masticatory muscles and the temporomandibular joint (TMJ). Abnormal mandibular movement in TMD patients may cause pain, chronic inflammation, and other discomfort, which could be relieved by a variety of drugs through various delivery systems. In this study, we summarized commonly used therapeutic agents in the management of TMD as well as novel bioactive molecules in preclinical stage and clinical trials. The emerging therapy strategies such as novel intra-TMJ delivery systems and implants based on tissue engineering are also discussed. This comprehensive review will strengthen our understanding of pharmacological approaches for TMD therapy.

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Co‑culture of bone marrow stromal cells and chondrocytes in�vivo for the repair of the goat condylar cartilage defects

Cited by 7 publications

References 43 publications

3D-Printed Polycaprolactone Reinforced Hydrogel as an Artificial TMJ Disc

3D-Printed Polycaprolactone Reinforced Hydrogel as an Artificial TMJ Disc

Tibial osteosynthesis with type II external skeletal fixator in a goat

Therapeutic Agents for the Treatment of Temporomandibular Joint Disorders: Progress and Perspective

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