Co‐culture of bone marrow‐derived mesenchymal stem cells overexpressing lipocalin 2 with HK‐2 and HEK293 cells protects the kidney cells against cisplatin‐induced injury

Abstract

Conditioned medium of mesenchymal stem cells (MSCs) is now being used for its cytoprotective effects, especially when the cells are equipped with cytoprotective factors to strengthen them against unfavorable microenvironments. Overexpression of Lcn2 in MSCs mimics in vivo kidney injury. Hence, unraveling how Lcn2-engineered MSCs affect kidney cells has been investigated. Cisplatin treated HK-2 or HEK293 kidney cells were co-cultivated with Lcn2 overexpressing MSCs in upper and lower chambers of transwell plates. Proliferation, apoptosis, and expression of growth factors and cytokines were assessed in the kidney cells. Co-cultivation with the MSCs-Lcn2 not only inhibited cisplatin-induced cytotoxicity in the HK-2 and HEK293 cells, but increased proliferation rate, prevented cisplatin-induced apoptosis, and increased expression of growth factors and the amount of antioxidants in the kidney cells. Thus Lcn2-engineered MSCs can ameliorate and repair injured kidney cells in vitro, which strongly suggests there are beneficial effects of the MSCs-Lcn2 in cell therapy of kidney injury.

In vitro differentiated hepatic oval‐like cells enhance hepatic regeneration in CCl4‐induced hepatic injury

“…In another study, ADSCs co‐cultured with normal human fibroblasts (NHF) enhanced the proliferation and wound healing of NHFs (Haubner et al, ). Cisplatin‐induced injury to kidney cells HEK 293 is reported to be protected by co‐culturing with MSCs and also enhances the proliferation of cells (Halabian et al, ). So, these diffent reports support our hypothesis as we have reported the reduction in the injury to hepatocytes by co‐culturing with BMSCs and BM‐OCs.…”

Protective effect of GDNF‐engineered amniotic fluid‐derived stem cells on the renal ischaemia reperfusion injury in vitro

“…Before the procedure, mRTECs at 4.0 × 10 5 cells/well were cultured in 6‐well plates and incubated at 37°C in a humidified atmosphere with 5% CO2 for 48 hours. At 80% confluence, the cells were washed with phosphate‐buffered saline (PBS) and incubated in paraffin at 37°C, 5% CO 2 for 90 minutes . Then we removed the paraffin, used PBS to eliminate non‐attached cells and added the fresh medium to the cells.…”

Potential advantages of acute kidney injury management by mesenchymal stem cells

“…Interestingly, similar results have been obtained in an in vitro model of AKI with a conditioned medium produced by genetically modified MSCs. MSCs were manipulated to over-express Lnc2; the conditioned medium produced from these cells was used to treat that cisplatin treated HEK 293 kidney cells in which it prevented apoptosis and increased the expression of growth factors, thus ameliorating and repairing injured cells [27] . MSCs secrete a number of factors, including VEGF, HGF, IGF-1, adrenomedullin, SDF-1, that exert antiapoptotic, mitogenic, vasoprotective, and angiogenic actions in AKI.…”