Co-culture Caco-2/Immune Cells

Kleiveland, C

doi:10.1007/978-3-319-16104-4_18

Cited by 18 publications

(19 citation statements)

References 29 publications

(11 reference statements)

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“…Intestinal or immune co-cultures, however, have not been used widely due to the lack of standardization in immune cells response, Table 3. Major intestinal epithelial cell types and their role in the intestinal environment (Furness et al, 2013;Johansson et al, 2013;Peterson and Artis, 2014) Major which depends on the donor and can lead to increased variability between experiments (Kleiveland, 2015).…”

Section: In Vitro Models Of the Intestinal Barriermentioning

confidence: 99%

Invited review: Dairy proteins and bioactive peptides: Modeling digestion and the intestinal barrier

Giromini

Cheli

Rebucci

et al. 2019

Journal of Dairy Science

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Dairy products are one of the most important sources of biologically active proteins and peptides. The healthpromoting functions of these peptides are related to their primary structure, which depends on the parent protein composition. A crucial issue in this field is the demonstration of a cause-effect relationship from the ingested protein form to the bioactive form in vivo. Intervention studies represent the gold standard in nutritional research; however, attention has increasingly been focused on the development of sophisticated in vitro models of digestion to elucidate the mechanism of action of dairy nutrients in a mechanistic way and significantly reduce the number of in vivo trials. On the other hand, the epithelial intestinal barrier is the first gate that actively interacts with digestion metabolites, making the intestinal cells the first target tissue of dairy nutrients and respective metabolites. An evolution of the in vitro digestion approach in the study of dairy proteins and derived bioactive compounds is the setup of combined in vitro digestion and cell culture models taking into consideration the endpoint to measure the target organism (e.g., animal, human) and the key concepts of bioaccessibility, bioavailability, and bioactivity. This review discusses the relevance and challenges of modeling digestion and the intestinal barrier, focusing on the implications for the modeling of dairy protein digestion for bioactivity evaluation.

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Section: In Vitro Models Of the Intestinal Barriermentioning

confidence: 99%

Invited review: Dairy proteins and bioactive peptides: Modeling digestion and the intestinal barrier

Giromini

Cheli

Rebucci

et al. 2019

Journal of Dairy Science

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“…Caco-2 cells, derived from human colon adenocarcinoma, are able to differentiate when cultured on a porous membrane, creating a functional polarized monolayer that can be used as a model of intestinal barrier. Co-culture models of Caco-2 cells with other cell lines have been proposed, including studies on their interactions with the immune system (10). These latter have been mainly focused on the response of Caco-2 to exogenous stimuli and how this is modified in presence of cells of the immune system (11,12).…”

Section: Introductionmentioning

confidence: 99%

Immunophenotyping Reveals No Significant Perturbation to PBMC Subsets When Co-cultured With Colorectal Adenocarcinoma Caco-2 Cells Exposed to X-Rays

et al. 2020

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Borsci et al. Immunophenotyping in PBMC/Caco-2 Co-culture washed-out by inter-individual differences). For this purpose, the model needs to be modified and further optimized e.g., including a pre-treatment strategy for PBMCs. We also performed a pooled analysis of all experimental observations with principal component analysis, suggesting the potential of this tool to identify subpopulations of similarly-responding donors.

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“…Ideally, an established in vitro system would have high expression of M cells. The Raji B cells are postulated to induce the expression of M cells from Caco-2 cells [15]. It is evident from the SEM results that the M cells were expressed in 3D coculture alginate hydrogel beads.…”

Section: Discussionmentioning

confidence: 88%

Expression of the Microfold Cells in Three-Dimensional Coculture System for in Vitro Cultivation of Human Norovirus

2019

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Introduction: Human Norovirus (HuNoV), a food-borne virus is the leading cause for acute gastroenteritis. However, its inability to propagate in vitropersists as major challenge in understanding HuNoV biology.Objective: This study aims to determine an effective culture system for HuNoV.Methods: The Caco-2 cells were cocultured with Raji B cells on alginate hydrogel beads. Scanning electron microscopy (SEM) was performed to confirmthe three-dimensional (3D) cells morphology. Western blot (WB) analysis was performed to detect protein markers expressed by Microfold (M) cells.Results: Optimization of Caco-2 cells monoculture in the alginate hydrogel beads showed optimum number of cells of 1 × 106 cells/ml, indicatedby the intact structure of the beads. Result of SEM showed clear structure of monoculture in the alginate hydrogel beads indicated by the presenceof smooth and regular apical surface while the coculture showed reduced apical surface of M cells. The result of WB showed downregulation ofUlex europaeus antibody expression.Conclusion: It is evident that the expression of M cells grown in 3D alginate hydrogel beads was successful, indicated by the structural morphologyseen under SEM as well as expression of protein marker by M cells. This established in vitro system is highly potential for cultivation of HuNoV.

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Co-culture Caco-2/Immune Cells

Cited by 18 publications

References 29 publications

Invited review: Dairy proteins and bioactive peptides: Modeling digestion and the intestinal barrier

Invited review: Dairy proteins and bioactive peptides: Modeling digestion and the intestinal barrier

Immunophenotyping Reveals No Significant Perturbation to PBMC Subsets When Co-cultured With Colorectal Adenocarcinoma Caco-2 Cells Exposed to X-Rays

Expression of the Microfold Cells in Three-Dimensional Coculture System for in Vitro Cultivation of Human Norovirus

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