Co-cultivation of siderophore-producing bacteria Idiomarina loihiensis RS14 with Chlorella variabilis ATCC 12198, evaluation of micro-algal growth, lipid, and protein content under iron starvation

Rajapitamahuni, Soundarya; Bachani, Pooja; Sardar, Raj Kumar; Mishra, Sandhya

doi:10.1007/s10811-018-1591-2

Cited by 47 publications

(18 citation statements)

References 44 publications

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“…Also, various recent reports in the growth pattern of different Chlorella sp. were in accordance with the results obtained from this study [13][14][15][16] . The cell density obtained in this study was higher since the culture was grown in ambient condition without CO 2 purging.…”

Section: Habitat Of Microalgaesupporting

confidence: 92%

First Report on Tertiary Amine as a Co2 Switchable Solvent for Hypersaline Trebouxiophycean Microalgae Towards Greener and Competent Lipid Extraction

Anto

Premalatha

Mathimani

2020

Preprint

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Considering the momentous cost drivers in energy efficient algal biorefinery processes, a green alternative in the lipid extraction process from microalgae is anticipated. Switchable solvent system using tertiary amines namely DMBA (Dimethylbenzylamine), DMCHA (Dimethylcyclohexylamine), and DIPEA (Diisopropylethylamine) for lipid extraction from wet hypersaline microalgae was investigated in this study. Interestingly, showed that at 1:1 (v/v of fresh DMBA solvent: microalgal biomass), and for 1 h extraction time, the lipid yield was 41.9, 26.6, and 33.3% for Chlorella sp. NITT 05, Chlorella sp. NITT 02, and Picochlorum sp. NITT 04 respectively and for recovered DMBA solvent at 1:1 (v/v) and for 1 hour extraction time, the lipid yield was 40.8, 25.97, and 32%, respectively. Similarly, lipid extraction using DMCHA solvent for Chlorella sp. NITT 05, Chlorella sp. NITT 02, and Picochlorum sp. NITT 04 at 1:1 (v/v of solvent: microalgal biomass) and 1 h extraction time showed 34.28, 24.24 and 23.33% lipids, respectively for fresh solvent and 34.01, 24.24 and 23.18% for recovered solvent respectively; while DIPEA was not competent in lipid extraction from three tested microalgae. FAME profile shows the presence of major saturated fatty acid as C16:0 (~ 30%) and major unsaturated fatty acid as C18:1 (~17%).

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Section: Habitat Of Microalgaesupporting

confidence: 92%

First Report on Tertiary Amine as a Co2 Switchable Solvent for Hypersaline Trebouxiophycean Microalgae Towards Greener and Competent Lipid Extraction

Anto

Premalatha

Mathimani

2020

Preprint

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“…For instance, it has been found that the microbial interaction between the bacterium Azospirillum brasilense and the microalga Chlorella vulgaris has a significant effect on fatty acid and lipid accumulation in the microalga ( Leyva et al, 2014 ). Additionally enhancement of microalgal growth, lipid and protein content of Chlorella variabilis was shown when the green microalga was co-cultivated with the siderophore-producing bacterium, Idiomarina loihiensis RS14, in an optimized ratio under iron-deficient conditions ( Rajapitamahuni et al, 2019 ). When microalga Chlorella was co-cultured with bacteria Bacillus firmus and Beijerinckia fluminensis to treat wastewater in vinegar production, though algal biomass was slightly decreased, a higher rate of nutrients removal was achieved ( Huo et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Global Metabolomics Reveals That Vibrio natriegens Enhances the Growth and Paramylon Synthesis of Euglena gracilis

Ouyang

Chen

Zhao

et al. 2021

Front. Bioeng. Biotechnol.

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The microalga Euglena gracilis is utilized in the food, medicinal, and supplement industries. However, its mass production is currently limited by its low production efficiency and high risk of microbial contamination. In this study, physiological and biochemical parameters of E. gracilis co-cultivated with the bacteria Vibrio natriegens were investigated. A previous study reports the benefits of E. gracilis and V. natriegens co-cultivation; however, no bacterium growth and molecular mechanisms were further investigated. Our results show that this co-cultivation positively increased total chlorophyll, microalgal growth, dry weight, and storage sugar paramylon content of E. gracilis compared to the pure culture without V. natriegens. This analysis represents the first comprehensive metabolomic study of microalgae-bacterial co-cultivation, with 339 metabolites identified. This co-cultivation system was shown to have synergistic metabolic interactions between microalgal and bacterial cells, with a significant increase in methyl carbamate, ectoine, choline, methyl N-methylanthranilate, gentiatibetine, 4R-aminopentanoic acid, and glu-val compared to the cultivation of E. gracilis alone. Taken together, these results fill significant gaps in the current understanding of microalgae-bacteria co-cultivation systems and provide novel insights into potential improvements for mass production and industrial applications of E. gracilis.

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“…interact each other to produce polysaccharises and exchange nutrients [11]. From our previous study [12] we found that C. variabilis was able to grow in the presence of Idiomarina loihiensis RS14 when inoculated in optimum conditions. But, with the increase of bacterial inoculum, microalgae growth started declining.…”

Section: Introductionmentioning

confidence: 94%

“…Estimation of standard siderophore on ROS activity C. variabilis grown in ZM was transferred in ask washed with HCl t.hat contained fresh media supplemented with standard siderophore. Both positive and negative control was prepared according to our previous work [12]. Puri ed siderophore ranging 10 µg, 30 µg, 50 µg, was added in iron de cient medium.…”

Section: Extraction and Puri Cation Of Siderophorementioning

confidence: 99%

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Exploitation of siderophore producing bacteria Acinetobacter soli (MTCC-5918) for lipids and reactive oxygen species in Chlorella variabilis (ATCC- PTA 12198) through co-cultivation

Rajapitamahuni

Bachani

Bharadwaj

et al. 2020

Preprint

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Background Increased iron uptake via siderophores triggers a series of physiological processes and generation of reactive oxygen species (ROS) which causes damage to proteins, lipids, carbohydrates, resulting into micro algal cell lysis. Moreover, there are reports mentioning oxidative stress is a mediator for increased lipid accumulation in microalgae. The main aim of this study is co-cultivation of the bacteria Acinetobacter soli (MTCC- 5918) and the microalgae Chlorella variabilis (ATCC-PTA 12198) under iron limiting conditions and the threshold value of iron that trigger oxidizing stress to microalgae. Further, the ROS generation in the microalgae C. variabilis was determined in terms of OH, SO2 and H2O2 concentration in the cells while co-cultivation. Results The co-cultured biomass contains (45.92 ± 0.74%) lipid content which was about 21% higher than that of the axenically grown microalgae. Carbohydrate content also increased to 40% than that of the control culture. Oxidative stress is a mediator for increased lipid accumulation in microalgae. As growth inhibition triggered due to the generation of high ROS toxicity during iron deficiency an increase in concentration of OH and H2O2 content was observed. In iron sufficient medium ash content of co-cultivated microalgae showed 32% and in iron deficient medium showed 14.23% which shows 44% decrease of ash content. Our novel approach significantly outperforms the involvement of different reactive oxygen species (ROS) in induction and in regulation of chelator release from cells at adequate Fe supply, which is also affecting the growth,lipids, carbohydrates, proteins, pigments, etc. Conclusions The findings of the present study highlights that oxidative stress is a mediator for increased lipid accumulation in microalgae that simultaneously becomes an alternative strategy for the improvement of biofuel potential in C. variabilis. The study portraysthe significance of co-cultivation of A. soli and C. variabilis induced oxidative stress (ROS generation) in microalgae caused due to higher uptake of iron via siderophore

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Co-cultivation of siderophore-producing bacteria Idiomarina loihiensis RS14 with Chlorella variabilis ATCC 12198, evaluation of micro-algal growth, lipid, and protein content under iron starvation

Cited by 47 publications

References 44 publications

First Report on Tertiary Amine as a Co2 Switchable Solvent for Hypersaline Trebouxiophycean Microalgae Towards Greener and Competent Lipid Extraction

First Report on Tertiary Amine as a Co2 Switchable Solvent for Hypersaline Trebouxiophycean Microalgae Towards Greener and Competent Lipid Extraction

Global Metabolomics Reveals That Vibrio natriegens Enhances the Growth and Paramylon Synthesis of Euglena gracilis

Exploitation of siderophore producing bacteria Acinetobacter soli (MTCC-5918) for lipids and reactive oxygen species in Chlorella variabilis (ATCC- PTA 12198) through co-cultivation

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