Co-cultivation of Phaeodactylum tricornutum and Aurantiochytrium limacinum for polyunsaturated omega-3 fatty acids production

Kadalag, Nikhil L.; Pawar, Pratik R.; Prakash, Gunjan

doi:10.1016/j.biortech.2021.126544

Cited by 19 publications

(5 citation statements)

References 39 publications

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“… 2.11 ± 0.17 0.08 ± 0.01 5.1 ± 0.3 0.19 ± 0.02 10 Schizochytrium sp. A-2 2.01 ± 0.20 0.13 ± 0.01 53.2 ± 0.30 3.44 ± 0.02 42 Nannochloropsis oceanica 4.12 ± 0.35 0.02 ± 0.01 2.37 ± 0.40 0.01 ± 0.01 47 Phaeodactylum tricornutum and Aurantiochytrium limacinum 17.96 ± 1.23 0.25 ± 0.01 18.42 ± 0.45 0.26 ± 0.01 48 Rhodomonas baltica NIVA-5/91 12.1 ± 1.07 0.88 ± 0.07 12.8 ± 0.28 0.95 ± 0.03 49 Phaeodactylum tricornutum CCMP 2561 26.2 ± 0.36 1.64 ± 0.05 4.93 ± 0.09 0.25 ± 0.02 49 Schizochytrium sp. HX-308 10.6 ± 0.34 2.25 ± 0.24 45.4 ± 0.95 9.59 ± 0.20 This study …”

Section: Resultsmentioning

confidence: 99%

Efficient co-production of EPA and DHA by Schizochytrium sp. via regulation of the polyketide synthase pathway

Liu

Zhang

et al. 2022

Commun Biol

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Presently, the supply of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) traditionally produced by marine fisheries will be insufficient to meet their market demand in food industry. Thus a sustainable alternative source is urgently required. Schizochytrium sp. is an ideal producer of DHA; however, its ability to co-produce DHA and EPA has not yet been proved. Herein, we first described a cobalamin-independent methionine synthase-like (MetE-like) complex, which contains independent acyltransferase and 3-ketoacyl synthase domains, independent of the traditional polyketide synthase (PKS) system. When the MetE-like complex was activated, the EPA content was increased from 1.26% to 7.63%, which is 6.06-folds higher than that in the inactivated condition. Through lipidomics, we find that EPA is more inclined to be stored as triglyceride. Finally, the EPA production was enhanced from 4.19 to 29.83 (mg/g cell dry weight) using mixed carbon sources, and the final yield reached 2.25 g/L EPA and 9.59 g/L DHA, which means that Schizochytrium sp. has great market potential for co-production of EPA and DHA.

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Section: Resultsmentioning

confidence: 99%

Efficient co-production of EPA and DHA by Schizochytrium sp. via regulation of the polyketide synthase pathway

Liu

Zhang

et al. 2022

Commun Biol

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“…Co-culturing microalgae with fungi has been recognized as a favorable strategy to solve this problem alternatively. For example, the culture system composed by P. tricornutum and A. limacinum produced EPA and DHA in advantageous ratios [ 155 ]. Carotenoids’ production is also enhanced when co-culturing fungi and microalgae.…”

Section: Co-culture Of Fungi-microalgae: the Biotechnological Usementioning

confidence: 99%

Fungal Contamination in Microalgal Cultivation: Biological and Biotechnological Aspects of Fungi-Microalgae Interaction

Laezza

Salbitani

Carfagna

2022

JoF

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In the last few decades, the increasing interest in microalgae as sources of new biomolecules and environmental remediators stimulated scientists’ investigations and industrial applications. Nowadays, microalgae are exploited in different fields such as cosmeceuticals, nutraceuticals and as human and animal food supplements. Microalgae can be grown using various cultivation systems depending on their final application. One of the main problems in microalgae cultivations is the possible presence of biological contaminants. Fungi, among the main contaminants in microalgal cultures, are able to influence the production and quality of biomass significantly. Here, we describe fungal contamination considering both shortcomings and benefits of fungi-microalgae interactions, highlighting the biological aspects of this interaction and the possible biotechnological applications.

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“…The traditional fish oil industries are based on fish stocks such as sardines, salmon, tuna, and herring [4]. Fish of the Salmonidae, Scombridae, and Clupeidae families have been the sole commercial sources of DHA as their oils contain approximately 20-30% DHA.…”

Section: Introductionmentioning

confidence: 99%

Investigation of Crypthecodinium cohnii High-Cell-Density Fed-Batch Cultivations

Dubencovs,

Suleiko,

Suleiko

et al. 2024

Fermentation

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Crypthecodinium cohnii is a marine microalga that can accumulate high amounts of polyunsaturated fatty acids (PUFAs) and thus replace conventional routes of fish oil production. They are associated with the destruction of marine resources and multiple downstream/purification complications. The major drawbacks of using C. cohnii for industrial-scale production are associated with low PUFA productivity. One of the means of increasing the PUFA synthesis rate is to maintain the medium component concentrations at optimal values throughout cultivation, thus increasing PUFA production efficiency, which can result in the successful transfer of the process to pilot and/or industrial scale. The goal of the present research was to develop techniques for increasing the efficiency of PUFA production via C. cohnii cultivation. Multiple experiments were carried out to test and fine-tune the cultivation medium composition and oxygen transfer factors. The biomass yields from individual components, yeast extract, sea salts, and glucose amounted to 5.5, 0.65, and 0.61 g·g−1, respectively. C. cohnii cell susceptibility to mechanical damage was experimentally evaluated. Power inputs of <276.5 W/m3 did not seem to promote cell destruction when Pitched-blade impellers were used. The obtained cultivation conditions were shown to be efficient in terms of increasing the biomass productivity and the omega-3 fatty acid content in C. cohnii. By using the applied methods, the maximal biomass productivity reached 8.0 g·L−1·day−1, while the highest obtained biomass concentration reached 110 g·L−1. A steady increase in the concentration of PUFAs during cultivation was observed from the FTIR data.

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Co-cultivation of Phaeodactylum tricornutum and Aurantiochytrium limacinum for polyunsaturated omega-3 fatty acids production

Cited by 19 publications

References 39 publications

Efficient co-production of EPA and DHA by Schizochytrium sp. via regulation of the polyketide synthase pathway

Efficient co-production of EPA and DHA by Schizochytrium sp. via regulation of the polyketide synthase pathway

Fungal Contamination in Microalgal Cultivation: Biological and Biotechnological Aspects of Fungi-Microalgae Interaction

Investigation of Crypthecodinium cohnii High-Cell-Density Fed-Batch Cultivations

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