Co-circulation of dengue virus serotypes in Central India: Evidence of prolonged viremia in DENV-2

Agarwal, Ankita; Gupta, Sudheer; Chincholkar, Tanvi; Singh, Vivek; Umare, Indresh Kumar; Ansari, Kudsia; Paliya, Sonam; Yadav, Abhishek; Chowdhary, Rashmi; Purwar, Shashank; Biswas, Debasis

doi:10.1016/j.meegid.2019.02.024

Cited by 8 publications

(3 citation statements)

References 43 publications

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“…Additionally, the MCC tree indicated that this lineage (4a) was introduced in Bhopal around 2013-2014. Previously, the introduction of a new genotype or lineage was proposed as the root cause of the transmission of a severe strain of dengue sickness in other parts of the world, such as the Americas (Rico-Hesse et al, 1997;Messer et al, 2003). It is pertinent to note that Bhopal experienced severe outbreaks of DENV in 2014 and 2016 (Agarwal et al, 2019), and since then the number of dengue cases has been increasing steadily in Bhopal (Sarma et al, 2022). This might be possible because lineages' extinction and inevitable incursion were connected to a virus's transmission bottleneck.…”

Section: Discussionmentioning

confidence: 99%

Transmission and Infection of Arboviruses

2024

Frontiers Research Topics

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Frontiers is more than just an open access publisher of scholarly articles: it is a pioneering approach to the world of academia, radically improving the way scholarly research is managed. The grand vision of Frontiers is a world where all people have an equal opportunity to seek, share and generate knowledge. Frontiers provides immediate and permanent online open access to all its publications, but this alone is not enough to realize our grand goals. Frontiers journal seriesThe Frontiers journal series is a multi-tier and interdisciplinary set of openaccess, online journals, promising a paradigm shift from the current review, selection and dissemination processes in academic publishing. All Frontiers journals are driven by researchers for researchers; therefore, they constitute a service to the scholarly community. At the same time, the Frontiers journal series operates on a revolutionary invention, the tiered publishing system, initially addressing specific communities of scholars, and gradually climbing up to broader public understanding, thus serving the interests of the lay society, too. Dedication to qualityEach Frontiers article is a landmark of the highest quality, thanks to genuinely collaborative interactions between authors and review editors, who include some of the world's best academicians. Research must be certified by peers before entering a stream of knowledge that may eventually reach the public -and shape society; therefore, Frontiers only applies the most rigorous and unbiased reviews. Frontiers revolutionizes research publishing by freely delivering the most outstanding research, evaluated with no bias from both the academic and social point of view. By applying the most advanced information technologies, Frontiers is catapulting scholarly publishing into a new generation. What are Frontiers Research Topics?Frontiers Research Topics are very popular trademarks of the Frontiers journals series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area.

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Section: Discussionmentioning

confidence: 99%

Transmission and Infection of Arboviruses

2024

Frontiers Research Topics

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“…Previously, the introduction of a new genotype or lineage was proposed as the root cause of the transmission of a severe strain of dengue sickness in other parts of the world, such as the Americas ( Rico-Hesse et al, 1997 ; Messer et al, 2003 ). It is pertinent to note that Bhopal experienced severe outbreaks of DENV in 2014 and 2016 ( Agarwal et al, 2019 ), and since then the number of dengue cases has been increasing steadily in Bhopal ( Sarma et al, 2022 ). This might be possible because lineages’ extinction and inevitable incursion were connected to a virus’s transmission bottleneck.…”

Section: Discussionmentioning

confidence: 99%

Molecular surveillance of dengue virus in field-collected Aedes mosquitoes from Bhopal, central India: evidence of circulation of a new lineage of serotype 2

Sarma,

Rathod,

Mishra

et al. 2023

Front. Microbiol.

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IntroductionDengue fever is hyperendemic in several Southeast and South Asian countries, including India, with all four serotypes (DENV 1–4) circulating at different periods and in different locations. Sustainable and improved virological and entomological surveillance is the only tool to prevent dengue and other vector-borne diseases.ObjectivesThe present study has been carried out to detect and characterize the circulating dengue virus (DENV) in field-collected Aedes mosquitoes in Bhopal, Central India.MethodsAedes mosquitoes were collected from 29 localities within Bhopal city during October 2020 to September 2022. DENV infection was assessed in the individual head and thorax regions of Aedes mosquitoes using reverse transcriptase PCR. Positive samples were sequenced, and the circulating serotypes and genotypes were determined using phylogenetic analysis.ResultsDENV RNA was detected in 7 Aedes aegypti and 1 Aedes albopictus, with infection rates of 0.59 and 0.14%, respectively. Phylogenetic analysis revealed all the isolates belonged to DENV serotype 2 and distinctly clustered with the non-Indian lineage (cosmopolitan genotype 4a), which was not recorded from the study area earlier. The time to most common recent ancestor (TMRCA) of these sequences was 7.4 years old, with the highest posterior density (HPD) of 3.5–12.2 years, indicating that this new lineage emerged during the year 2014. This is the first report on the DENV incrimination in both Ae. aegypti and Ae. albopictus mosquitoes collected from Bhopal, Central India.ConclusionThe observed emergence of the non-Indian lineage of DENV-2 in Bhopal, which again is a first report from the area, coincides with the gradual increase in DENV cases in Bhopal since 2014. This study emphasizes the importance of DENV surveillance and risk assessment in this strategically important part of the country to decipher its outbreak and severe disease-causing potential.

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“…Notably, shift of dominant serotypes from DENV-2 in 2013 to DENV-3 in 2016 occurred in India. Moreover, In 2017, studies conducted in the country revealed that DENV-2 and 3 were dominant in the southern region, while DENV-1 and 2 were prevalent in the northern region (Agarwal et al, 2019; Ganeshkumar et al, 2018; Gupta & Ballani, 2014). Recently, the shifting of predominant serotypes also observed in Thailand (from DENV-2 and DENV-4 to DENV-3), (Hamel et al, 2019) Sri Lanka (from DENV-1 to DENV-2) (Ali et al, 2018), Myanmar (from predominant serotype 1 to co-dominant serotypes 1, 2, and 4) (Oo et al, 2017), Indonesia, Bhutan (from DENV-3 to DENV-1), and in Nepal (Kusmintarsih et al, 2018; Prajapati et al, 2020; Zangmo et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Comparative Phylogenetic Analysis and Transcriptomic Profiling of Dengue (DENV-3 genotype I) Outbreak in 2021 in Bangladesh

Sarkar

Rahman²,

Islam

et al. 2022

Preprint

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Objectives: The lack of a dengue disease animal model and the complex immune interaction in dengue infection hinders the study of host response and immunopathogenesis. The development of next-generation sequencing (NGS) technology allowed the researchers to study the transcriptomic profiles of human in-depth. We, therefore, implicated phylodynamic and transcriptomic approaches through NGS technology to know the origin of dengue virus (DENV) and their host response in infected patients with dengue fever. Methods: To determine the whole genome sequences of the dengue virus and their transcriptomic profiles, RNA was extracted from the serum samples of 3 healthy, and 21 dengue patients. These samples were custom performed at phylogenetic, phylodynamic, differential express gene (DEG), and gene ontology (GO) using respective bioinformatics tools. Results: The whole genome sequence analysis revealed that the total number of nucleotide ranges on these serum RNA samples were in between 10647 and 10707. Phylogenetic tree analysis showed that these strains were belonged to DENV-3 genotype I. Phylodynamic analysis showed that the 2021 epidemic isolates were clade shifted and maintained as a new clade in compared to 2019 epidemic. Transcriptome analysis mapped a total of 19267 expressed genes. Of them, there were higher expression of genes in dengue-positive samples (n = 17375) with a count of 6953 unique genes in comparison to healthy controls (n = 12314) with a count of 1892 unique genes. A total of 2686 DEGs were identified in a host factor-independent manner in dengue patients with a q-value < 0.05. DESeq2 plot counts function of the top 24 genes with the smallest q-values of differential gene expression of RNA-seq data showed that 11 genes were upregulated, whereas 13 genes were downregulated. GO analysis showed a significant upregulation (p = <0.001) in a process of multicellular organismal, nervous system, sensory perception of chemical stimulus, and G protein-coupled receptor signalling pathways in the dengue patients. However, there were a significant downregulation (p = < 0.001) of intracellular component, cellular anatomical entity, and protein-containing complex in dengue patients. Most importantly, there was significant increase of a classes of immunity protein (Cytokines especially TGF-beta;1, chemokines, inflammasome, and factors for blood coagulations) in dengue patients in compared to the healthy controls, with increased GO of immune system process. In addition, upregulation of toll receptor (TLR) signalling pathways were also initiated in the patients infected with dengue virus. These TLR pathways were particularly involved for the activation of innate in couple with adaptive immune system that probably involved the rapid elimination of dengue virus infected cells. These differentially expressed genes could be further investigated for target based prophylactic interventions for dengue. Conclusion: This is a first report to document the complete genomic features of dengue, and differentially expressed genes in patients with dengue virus in Bangladesh. These genes may have diagnostic and therapeutic values for dengue infection. Continual genomic surveillance is required to further investigate the shift in dominant genotypes in relation to viral pathogenesis.

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Co-circulation of dengue virus serotypes in Central India: Evidence of prolonged viremia in DENV-2

Cited by 8 publications

References 43 publications

Transmission and Infection of Arboviruses

Transmission and Infection of Arboviruses

Molecular surveillance of dengue virus in field-collected Aedes mosquitoes from Bhopal, central India: evidence of circulation of a new lineage of serotype 2

Comparative Phylogenetic Analysis and Transcriptomic Profiling of Dengue (DENV-3 genotype I) Outbreak in 2021 in Bangladesh

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